Study of residence times distribution in a tubular reactor for the hydrolisis of soybean lecithin using immobilized A2 phospholipase
Keywords:A<sub>2</sub> phospholipase, Residence times, Tubular reactor
The hydrolisis of soybean lecithin can be carried out by means of the use of immobilized A2 phospholipase which releases a fatty acid of C-2 position of the phospholipids so that an enriched product in lysolecithins is obtained. The enzymatic reaction follows a first order kinetics when the substrate concentrations are in the range: 6.34•10-3 M to 19.0•10-3 M. The value of the rate constant: k= 9.88•10-2 min-1 corresponds to the one obtained for the immobilized enzyme on alumina. A reactor was constructed and alumina was the selected support because of its good mechanic properties and fundamentally because of its low cost. The flow behaviour in the reactor and how it departs from the ideal model of plug-flow was analyzed by injecting a NaCl solution of a well-known concentration (tracer) and then, passing it through the reactor. According to the experiences carried out, the conductivity measures proved adequate for the determination of the residence times. The system showed lineal behaviour. The residence times in the experimentally built reactor for different load arrangements (particle support + inert load) was analyzed by using three different flows. The nonconverted fractions for the reactor were calculated and differences in the output were observed, in comparison to the plug-flow reactor, precisely because of channelizations and shut-offs that are generated inside the column. Maximal conversion in the experiences carried out both with higher substrate concentrations and for a minor feed flow were achieved. The dispersion module resulted quite higher than the limit that introduces a gaussian curve, for the one for which the degree of supposition of high dispersion was correct. The reactor showed a behaviour similar to that of a reactor of complete mixture and it was concluded that the degree of back-mixing, the formation of whirls and zones of redistribution of material are important.
How to Cite
Copyright (c) 2001 Consejo Superior de Investigaciones Científicas (CSIC)
This work is licensed under a Creative Commons Attribution 4.0 International License.© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.