Probiotic characteristics of Lactobacillus plantarum strains from traditional butter made from camel milk in arid regions (Sahara) of Algeria
DOI:
https://doi.org/10.3989/gya.2008.v59.i3.511Keywords:
Antibiotic, Bacteriocin, Butter, Lactic acid bacteria, Lactobacillus plantarum, ProbioticsAbstract
Lactobacillus plantarum strains previously isolated from the traditional butter made from camel milk (shmen) were evaluated for some probiotic criteria. Among 38 strains investigated for bile resistance, 14 were tolerant to 2% oxgall with survival percentages ranging from 69 to 75%. Out of these, only 4 strains (L. plantarum SH5, SH12, SH24 and SH32) were sufficiently resistant to pH 2.0 for 2 to 6 h incubation periods. Only crude extracts of L. plantarum SH12 and L. plantarum SH24 were inhibitory against Lactococcus lactis B8, the strain used as indicator. The antibacterial activity of crude extracts was completely lost after treatment with α-chymotrypsin and proteinase K. L. plantarum SH12 and SH24 strains were susceptible to penicillin G, oxacillin, vancomycin and clindamycin, but resistant to tetracycline and kanamycin. These strains showed rapid acidification activity (0.921 and 1.075 mmol/l of lactic acid, respectively), a good proteolytic activity (5.45 and 3.49 mg/l tyrosine at 72 h, respectively) and high survival percentage after freeze-drying. None of the strains produced polysaccharides or haemolysin in sheep blood.
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Ayad EHE, Nashat S, El-Sadek N, Metwaly H, El-Soda M. 2004. Selection of wild lactic acid bacteria isolated from traditional Egyptian dairy products according to production and technological criteria. Food Microbiol. 21, 715-725. doi:10.1016/j.fm.2004.02.009
Conway PL, Gorbach SL, Golden BR. 1987. Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J. Dairy Sci. 70, 1–12.
Coppola R, Succi M, Tremonte P, Reale A, Salzano G, Sorrentino E. 2005. Antibiotic susceptibility of L. rhamnosus strains isolated from Parmigiano Reggiano cheese. Lait 85, 193-204. doi:10.1051/lait:2005007
de Man JC, Rogosa M, Sharpe EM. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23, 130-135.
Delgado A, Brito D, Fevereiro P, Marques JF. 2001. Antimicrobial activity of L. plantarum, isolated from a traditional lactic acid fermentation of table olives. Lait 81, 203-215. doi:10.1051/lait:2001124
Dunne C, Murphy L, Flynn S, O’Mahony L, O’Halloran S, Feeney M, Morrissey D, Thornton G, Fitzgerald G, Daly C, Kiely B, Quigley E MM, O’Sullivan GC, Shanahan F, Kevin J. 1999. Probiotics: from myth to reality. Demonstration of functionality in animal models of disease and in human clinical trials. Antonie van Leeuwenhoek 76, 279-292. doi:10.1023/A:1002065931997 PMid:10532384
Dunne C, O’Mahony L, Murphy L, Thornton G, Morrissey D, O’Halloran S, Feeney M, Flynn S, Fitzgerald G, Daly C, Kiely B, O’Sullivan GC, Shanahan F, Collins JK. 2001. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am. J. Clin. Nutr. 73 (suppl), 386S–392S.
El-Naggar MYM. 2004. Comparative study of Probiotic cultures to control the growth of E. coli O157:H7 and Salmonella tybhimurium. Biotechnology 3, 173-180.
FAO. 2003. Production et santé animales. Lait de Chamelle pour l’Afrique. Niamey, 5 - 8 Novembre.
Farah Z, Streiff T, Bachmann MR. 1989. Manufacture and characterization of camel milk butter. Milchwissenschaft 44, 412-414.
Font de Valdez G, Savoy de Giori G, Pesce de Ruiz Holgado AA, Oliver G. 1985. Effect of the rehydration medium on the recovery of freeze-dried lactic acid bacteria. Appl. Environ. Microbiol. 50, 1339-1341.
Geis A, Singh J, Teuber M. 1983. Potential of lactic streptococci to produce bacteriocin. Appl. Environ. Microbiol. 45, 205-211.
Gotcheva V, Hristozova E, Hristozova T, Guo M, Roshkova Z, Angelov A. 2002. Assessment of potential probiotic properties of lactic acid bacteria and yeast strains. Food Biotechnol. 16, 211-225. doi:10.1081/FBT-120016668
Halami PM, Chandrashekar A, Nand K. 2000. Lactobacillus farciminis MD, a newer strain with potential for bacteriocin and antibiotic assay. Letters Appl. Microbiol. 30, 197–202. doi:10.1046/j.1472-765x.2000.00691.x PMid:10747250
Hammes WP, Hertel C. 2003. The genera Lactobacillus and Carnobacterium, in Dworkin M et al. (Eds.) The Prokaryotes: An evolving electronic resource for the microbiological community. Release 3.15. Springer-Verlag, New York, http://www.springerlink.com/content/pq0t51077605kr21/
Hull ME. 1947. Studies of milk proteins. Colorimetric determination of the partial hydrolysis of the proteins in milk. J. Dairy Sci. 30, 881.
IDF (International Dairy Federation) standard 149A. 1997. Levains lactiques de cultures de bacteries lactiques. Norme de composition. IDF Standard 149A. Brussels, Belgium.
Kacem M, Karam NE. 2006a. Physicochemical and microbiological study of “shmen”, a traditional butter made from camel milk in the Sahara (Algeria): isolation and identification of lactic acid bacteria and yeasts. Grasas y Aceites 57 (2) 198-204.
Kacem M, Karam NE. 2006b. In vitro preselection criteria for probiotic Lactobacillus plantarum strains of fermented olives origin. Int. J. Probiotics Prebiotics (in press).
Kacem M, Zadi-Karam H, Karam N. 2005. Detection and activity of plantaricin OL15, a bacteriocin produced by Lactobacillus plantarum isolated from Algerian fermented olives. Grasas y Aceites 56, 192-197.
Klaenhammer TR. 1988. Bacteriocins of lactic acid bacteria. Biochimie 70, 337-349. doi:10.1016/0300-9084(88)90206-4 PMid:3139051
Lankaputhra WEV, Shah NP. 1995. Survival of Lactobacillus acidophilus and Bifidobacterium spp in the presence of acid and bile salts. Cultured Dairy Prod. J. 30, 2–7.
Lombardi A, Dal Maistro L, De Dea P, Gatti M, Giraffa G, Neviani E. 2002. A polyphasic approach to highlight genotypic and phenotypic diversities of Lactobacillus helveticus strains isolated from diary starter cultures and cheeses. J. Dairy Res. 69, 139–149. doi:10.1017/S0022029901005349 PMid:12047105
Lourens-Hattingh A, Viljoen BC. 2001.Yogurt as probiotic carrier food. Int. Dairy J. 11, 1–17. doi:10.1016/S0958-6946(01)00036-X
Maldonado A, Ruiz-Barba JL, Jiménez-Díaz R. 2003. Production of plantaricin NC8 by Lactobacillus plantarum NC8 is induced in the presence of different types of gram positive bacteria. Arch. Microbiol. 181, 8-16. doi:10.1007/s00203-003-0606-8 PMid:14647979
Mora D, Fortina MG, Parini C, Ricci G, Gatti M, Giraffa G, Manachini PL. 2002. Genetic diversity and technological properties of Streptococcus thermophilus strains isolated from dairy products. J. Appl. Microbiol. 93, 278–287. doi:10.1046/j.1365-2672.2002.01696.x PMid:12147076
NCCLS (National Committee for Clinical Laboratory Standards). 1999. NCCLS document M-100-S9. Performance Standards for antimicrobial susceptibility testing. 9 th. information supplement. NCCLS, Wayne, PA.
Pryce JD. 1969. A modification of the Barker-Summerson method for the determination of lactic acid. Analyst 94, 1151-1152. doi:10.1039/an9699401151 PMid:5358920
Ramet JP. 1991. La transformation en fromages de lait de dromadaire.World Animal Rev. 67, 20-28.
Rao MB, Gupta RC, Dastur NN. 1970. Camels’ milk and milk products. Ind. J. Dairy Sci. 23, 71–78,
Salminen S, Wright A, Morelli L, Marteau P, Brassart D, de Vos WM, Fonden R, Saxelin M, Collins K, Mogensen G, Birkeland S-E, Mattila-Sandholm T. 1998. Demonstration of safety of probiotics, a review. Int. J. Food Microbiol. 44, 93–106. doi:10.1016/S0168-1605(98)00128-7 PMid:9849787
Schillinger U, Lücke FK. 1989. Antibacterial activity of L. sake isolated from meat. Appl. Environ. Microbiol. 55, 1901-1906.
Tannock GW, Munro K, Harmsen HJ, Welling GW, Smart J, Gopal PK. 2000. Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20. Appl. Environ. Microbiol. 66, 2578-2588. doi:10.1128/AEM.66.6.2578-2588.2000 PMid:10831441 - PMCid:110584
Thomas TD, Pritchard GG. 1987. Proteolytic enzymes of dairy starter cultures. FEMS Microbiol. Rev. 46, 245–268. doi:10.1111/j.1574-6968.1987.tb02464.x
Toba T, Samant SK, Yoshioka E, Itoh T. 1991. Reutericin 6, a new bacteriocin produced by Lactobacillus reuteri LA 6. Letters Appl. Microbiol. 13, 281-286. doi:10.1111/j.1472-765X.1991.tb00629.x
Usman M, Hosono A. 1999. Bile tolerance taurocholate desegregation and binding of cholesterol by Lactobacillus gasseri strain. J. Appl. Sci. 82, 243-248.
Xanthopoulos V, Litopoulou-Tzanetaki E, Tzanetakis N. 1997.In vitro study of Lactobacillus species strains on bile tolerance and cholesterol removal, in Lactic Acid Bacteria – Lactic 97. Caen, Presses Universitaires de Caen.
Yagil R. 1982. Camels and camel milk, in FAO Animal Production and Health Paper, no. 26. FAO, Rome.
Sharmanov TSh, Kadyrova RKh, Shlygina OE, Zhaksylykova RD. 1978. Changes in the indicators of radioactive isotope studies of the liver of patients with chronic hepatitis during treatment with whole camels’ and mares’ milk. Voprosy Pitaniya 1, 9–13.
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