Optimization of the recovery yield of the enzymatic aqueous extraction of oil from wet açaí decocts using Design of Experiment

B. Rodrigues; C. Herman

doi:10.3989/gya.0981221

Autores/as

B. Rodrigues Centre for Valorization of Amazonian Bioactive Compounds (CVACBA), Universidade Federal do Pará (UFPA)
C. Herman Centre for Valorization of Amazonian Bioactive Compounds (CVACBA), Universidade Federal do Pará (UFPA)

DOI:

https://doi.org/10.3989/gya.0981221

Palabras clave:

CCD metodología de superficie de respuesta, Diseño de mezclas, Euterpe oleracea

Resumen

En las últimas décadas, el aceite de açaí ha sido ampliamente estudiado por sus propiedades biológicas, resultando interesante para las industrias relacionadas con la salud. Por lo tanto, se ha vuelto necesario desarrollar técnicas de extracción ecológicas. El objetivo principal de este estudio es el uso de diseños experimentales para la maximización del rendimiento en la recuperación del proceso de extracción acuosa enzimática del aceite de açaí a partir de cocciones. Se emplea un diseño de mezcla simplex-lattice para la optimización de las proporciones de tres preparaciones enzimáticas comerciales. Posteriormente, se utiliza un Diseño Compuesto Central para identificar los valores óptimos de la concentración enzimática total (0,5-4,5%) y el tiempo de extracción (2-12h). La “Metodología de superficie de respuesta” (RSM) señala que el rendimiento máximo (60,55 ± 5,98%) se obtiene utilizando una mezcla ternaria 0,49:0,25:0,28 de Celluclast 1,5 L, Viscozyme L y Ultrazym AFP-L a una concentración enzimática total de 2,85% durante 10,9 horas. Este estudio indica que la extracción acuosa enzimática del aceite de açaí es un proceso eficiente y sostenible.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Abdulkarim SM, Lai OM, Muhammad SKS, Long K, Ghazali HM. 2006. Use of enzymes to enhance oil recovery during aqueous extraction of moringa oleifera seed oil. J. Food Lipids 13, 113-130. https://doi.org/10.1111/j.1745-4522.2006.00038.x

Batista CCR, Oliveira MS, Araújo ME, Rodrigues AMC, Botelho JRS, Filho APSS, Machado NT, Junior RNC. 2019. Supercritical CO2 extraction of açai (Euterpe oleracea) berry oil: Yield fatty acids allelopathic activities and determination of phenolic and anthocyanins total compounds in the residual pulp. J. Supercrit. Fluids 107, 364-369. https://doi.org/10.1016/j.supflu.2015.10.006

Bichara CMG, Rogez H. 2011. Chapter 1-Açai (Euterpe oleracea Martius) In: Postharvest biology and technology of tropical and subtropical fruit: Açai to Citrus Cambridge: UK Press pp 1-26. https://doi.org/10.1533/9780857092762.1

Darnet S, Serra JL, Rodrigues AMC, Silva LHM. 2011. A high-performance liquid chromatography method to measure tocopherols in assai pulp (Euterpe oleracea). Food Res. Int. 44, 2107-2111. https://doi.org/10.1016/j.foodres.2010.12.039

Dela Cruz FB, Migo VP, Valência SA, Demafelis RB, Alfafara CG, Alcantara AJ. 2007. Enzyme mixture for the extraction of oil from seed of Vutalao (Calophyllum inophyllum). Philipp J. Crop Sci. 32 (3), 17-30.

Domínguez H, Núnez MJ, Lema JM. 1994. Enzymatic pretreatment to enhance oil extraction from fruit and oilseeds: A review. Food Chem. 49, 271-286. https://doi.org/10.1016/0308-8146(94)90172-4

Ferreira ES, Rogez HLG, Herman CANP. 2018 Effect of the combination of enzymatic preparations on the aqueous extraction yield of the oil from the pulp of Euterpe oleracea fruit. Braz. J. Chem. Eng. 35 (4), 1193-1201. https://doi.org/10.1590/0104-6632.20180354s20170305

Li H, Song C, ZhouH, Wang N, Cao D. 2010. Optimization of the aqueous enzymatic extraction of wheat germ oil using Response Surface Methodology. J. Am. Oil Chem. Soc. 88 (6), 809-817. https://doi.org/10.1007/s11746-010-1731-6

Liu C, Hao L, Chen F, Yang C. 2020. Study on extraction of peanut protein and oil bodies by aqueous enzymatic extraction and characterization of protein. J. Chem. 2020, ID 5148967 11 p. https://doi.org/10.1155/2020/5148967

Magalhães TSSA, Macedo PCO, Converti A, Lima AAD. 2020 The use of Euterpe oleracea Mart. as a new perspective for disease treatment and prevention. Biomolecules 10 (6), 813. https://doi.org/10.3390/biom10060813 PMid:32466439 PMCid:PMC7356995

Lubrano C, Robin JR, Khaiat. 1994. A Fatty acids sterol and tocopherol composition of oil from the fruit mesocarp of six palm species in French Guiana. OCL.

Mai HC, Truong V, Debaste F. 2013. Optimization of enzyme-aided extraction of oil rich in carotenoids from gac fruit (Momordica cochinchinensis Spreng). Food Technol. Biotech. 51 (4), 488-499.

Nascimento RJS, Curi S, Assis RA, Freitas SP. 2008. Composição em ácidos graxos do óleo da polpa de açaí extraído com enzimas e com hexano. Rev Bras Frutic 30(2):498-502. https://doi.org/10.1590/S0100-29452008000200040

Nguyen HC, Vuong DP, Nguyen NTT, Nguyen NP, Su C-H, Wang F-M, Juan H-J. 2020. Aqueous enzymatic extraction of polyunsaturated fatty acid-rich sacha inchi (Plukenetia volubilis L.) seed oil: An eco-friendly approach. LWT 133, 109992. https://doi.org/10.1016/j.lwt.2020.109992

Pacheco-Palencia LA, Talcott SM, Talcott ST. 2008. Chemical composition antioxidant properties and thermal stability of a phytochemical enriched oil from açai (Euterpe oleracea Mart). J Agric. Food Chem. 56, 4631-4636. https://doi.org/10.1021/jf800161u PMid:18522407

Ricochon G, Muniglia L. 2010. Influence of enzymes on the oil extraction processes in aqueous media. OCL 17 (6), 356-359. https://doi.org/10.1051/ocl.2010.0337

Rosenthal A, Pyle DL, Niranjan K. 1996. Aqueous and enzymatic process for edible oil extraction. Enzyme Microb. Technol. 19, 402-420. https://doi.org/10.1016/S0141-0229(96)80004-F

Silva MP, Cunha VMB, Sousa SHB, Menezes EGO, Bezerra PN, Neto JTF, Filho GNR, Araújo ME, Carvalho Jr RN. 2019. Supercritical CO2 extraction of lyophilized Açaí (Euterpe oleracea Mart.) pulp oil from three municipalities in the state of Pará, Brazil. J. CO 2 Utilization 31, 226-234. https://doi.org/10.1016/j.jcou.2019.03.019

Schauss AG. 2010. Açai (Euterpe oleracea Mart): A macro and nutrient rich palm fruit from the Amazon rain forest with demonstrated bioactivities in vitro and in vivo. In D R Watson & V R Preedy (Eds) Bioactive foods promotion health (pp 479-490) Oxford: Academic Press. https://doi.org/10.1016/B978-0-12-374628-3.00032-3

Silvamany H, Jahim JM. 2015. Enhancement of palm oil extraction using cell wall degrading enzyme formulation. Malaysian J. Anal. Sci. 19 (1), 77-87. https://doi.org/10.11113/jt.v77.7016

Skrovankova S, Sumczynski D, Mlcek J, Jurikova T, Sochor J. 2015 Bioactive compounds and antioxidant activity in different types of berries. Int. J. Mol. Sci. 16 (10), 24673-24706. https://doi.org/10.3390/ijms161024673 PMid:26501271 PMCid:PMC4632771

Tabtabaei S, Diosady LL. 2013. Aqueous and enzymatic extraction processes for the production of food-grade proteins and industrial oil from dehulled yellow mustard flour. Food Res. Int. 52, 547-556. https://doi.org/10.1016/j.foodres.2013.03.005

Womeni HM, Ndjouenkeu R, Kapseu C, Mbiapo FT, Parmentier M, Fanni J. 2008. Aqueous enzymatic oil extraction from Irvingia gabonensis seed kernels. Eur. J. Lipid Sci. Techno.l 110, 232-238. https://doi.org/10.1002/ejlt.200700172

Xie M, Dunford NT, Goad C. 2011. Enzymatic extraction of wheat germ oil. J. Am. Oil Chem. Soc. 88, 2015-2021. https://doi.org/10.1007/s11746-011-1861-5

Yamaguchi KKL, Pereira LFR, Lamarão CV, Lima ES, Veiga-Junior VF. 2015. Amazon açaí: Chemistry and biological activities: A review. Food Chem. 179, 137-151. https://doi.org/10.1016/j.foodchem.2015.01.055 PMid:25722148

Yusoff MM, Gordon MH, Niranjan K. 2015 Aqueous enzyme assisted oil extraction from oilseeds and emulsion de-emulsifying methods: A review. Trends Food Sci. Technol. 41, 60-82. https://doi.org/10.1016/j.tifs.2014.09.003

Zhang S, Wang Z, Xu SY.2007. Optimization of the aqueous enzymatic extraction of rapeseed oil and protein hydrolysates. J. Am. Oil Chem. Soc. 84, 97-105. https://doi.org/10.1007/s11746-006-1004-6