Antioxidant evaluation and chemoprotection of phenolic extracts from apple seeds

Authors

  • R. F. González-Laredo Instituto Tecnológico de Durango, Dpto. de Ingenierías Química y Bioquímica
  • M. G. Reyes-Navarrete Instituto Tecnológico de Durango, Dpto. de Ingenierías Química y Bioquímica
  • A. M. Preza y Lerma Instituto Tecnológico de Durango, Dpto. de Ingenierías Química y Bioquímica
  • M. Rosales-Castro Instituto Tecnológico de Durango, Dpto. de Ingenierías Química y Bioquímica
  • J. Morales-Castro Instituto Tecnológico de Durango, Dpto. de Ingenierías Química y Bioquímica
  • J. A. Gallegos-Infante Instituto Tecnológico de Durango, Dpto. de Ingenierías Química y Bioquímica
  • N. E. Rocha-Guzmán Instituto Tecnológico de Durango, Dpto. de Ingenierías Química y Bioquímica

DOI:

https://doi.org/10.3989/gya.2007.v58.i1.1

Keywords:

Antioxidants, Apple, Chemoprotection, Polyphenol, Seeds

Abstract


Phenolic extracts from apple seeds (Malus domestica) belonging to the Red delicious (Rd) and Blanca de asturias (Ba) varieties were studied. Two extraction sequences with organic solvents were performed to evaluate hydrophilic phytochemicals: one with hexane, ethyl acetate and 50% methanol and the other with hexane and 70% acetone. For both apple varieties, acetone extracts showed higher total phenolics and condensed tannin content than ethyl acetate or aqueous methanol extracts. The same trend was observed with acetone extracts, which showed the highest free radical scavenging activity (%RSA). Bidimensional thin later chromatography plates gave positive evidence for proanthocyanidins (condensed tannins). The effect of crude  extracts on transformed cells (HeLa) was evaluated and found to be as strong as the positive control (catechin). Aqueous acetone extracts showed the highest inhibition to cell proliferation of all tested extracts and controls.

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References

Balasubramanian S, Efimova T, Eckert R L. 2002. Green tea polyphenol stimulates a Ras, MEKK1, MEK3, and p38 cascade to increase activator protein 1 factor-dependent involucrin gene expresión in normal human keratinocytes. J. Biol. Chem. 277, 1828-1836. doi:10.1074/jbc.M110376200

Brand-Williams WB, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensm-Wiss.u.Technol. 28, 25-30.

Chung KT, Wong TY, Wei Ch, Huang YW, Lin Y. 1998.Tannins and Human Health: a Review. Critical Reviews in Food Science and Nutrition. 38, 421-464. doi:10.1080/10408699891274273

Cordell, GA, Kinghorn, AD, Pezzuto, JM. 1993. Separation, structure elucidation and bioassay of cytotoxic natural products. In: Bioactive natural products: detection, isolation and structure determination, 199-200. Colegate, S. M., Molyneux, R. J. (Eds.). CRC Press.

Eberhardt MV, Lee CY, Liu RH. 2000. Antioxidant activity of fresh apples. Nature.405, 903-904.

Halvorsen BL, Holte K, Myhrstad MCW, Barikmo I, Hvattum E, Remberg SF, Wold AB, Haffner K, Baugerod H, Andersen LF, Moskaug JO, Jacobs DR, Blomhoff R. 2002. A systematic screening of total antioxidants in dietary plants. J. Nutr. 132, 461-471.

Karchesy JJ, Bae Y, Chalder-Scott L, Helm RF, Yeap Foo L. 1989. Chromatography of proanthocyanidins. In: Chemistry and Significance of Condensed Tannins. Karchesy J. J., Hemingway R. W., 139-151. Plenum press.

Lees GL, Wall KM, Beveridge TH, Suttill NH. 1995. Localization of condensed tannins in apple fruit peel, pulp and seeds. Can. J. Bot. 73, 1897-1904. doi:10.1139/b95-202

Laux MT, Ketzis J, Berry J. 2002. Plants natural products as source of anti-cancer drugs, a review. Revista Latinoamericana de Química 30, 29-36.

Lu Y, Foo LY. 1998. Constitution of some chemical components of apple seed. Food Chem. 61, 29-33. doi:10.1016/S0308-8146(97)00123-4

Lu Y, Foo LY. 2000. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68, 81-85. doi:10.1016/S0308-8146(99)00167-3

Markham KR. 1982. Techniques of Flavonoid Identification. Academic Press, New York, USA.

Middleton E, Kandaswami Ch, Theoharides TC. 2000. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacological Reviews 52, 673-751.

Pekkarinen SS, Stockmann H, Schwarz K. 1999. Antioxidant activity and partitioning of phenolic acids in bulk and emulsified methyl linoleate. J. Agric. Food Chem. 47, 3036-3043. doi:10.1021/jf9813236

Sakakibara H, Honda Y, Nakagawa S, Ashida H, Kanazawa K. 2003. Simultaneous determination of all polyphenols in vegetables, fruits and teas. J. Agric. Food Chem. 51, 571-581. doi:10.1021/jf020926l

Sanchez-Moreno C, Larrauri JA, Saura-Calixto F. 1999. Free radical scavenging capacity and Inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Res. International. 32, 407-412. doi:10.1016/S0963-9969(99)00097-6

Saura-Calixto F. 1998. Antioxidant dietary fiber product: A new concept and a potential food ingredient. J. Agric. Food Chem. 46, 10-15. doi:10.1021/jf9803841

Waterman PG, Mole S. 1994. Analysis of Phenolic Plant Metabolites. Blackwell Scientific Publications, England.

Wolfe KL, Wu X, Liu RH. 2003. Antioxidant activity of apple peels. J. Agric. Food Chem. 51, 609-614. doi:10.1021/jf020782a

Yazaky Y, Gu R, Lin Y, Chen N, Nguyen NK. 1993. Analyses of black wattle tannins (Acacia mearnsii). Holzforschung. 47, 57-61.

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Published

2007-03-30

How to Cite

1.
González-Laredo RF, Reyes-Navarrete MG, Preza y Lerma AM, Rosales-Castro M, Morales-Castro J, Gallegos-Infante JA, Rocha-Guzmán NE. Antioxidant evaluation and chemoprotection of phenolic extracts from apple seeds. Grasas aceites [Internet]. 2007Mar.30 [cited 2025Feb.22];58(1):5-9. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1

Issue

Vol. 58 No. 1 (2007)

Section

Research

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