Measurement of the total hydroperoxides in ozonated sunflower oil using the ferrous oxidation in xylenol orange assay

Authors

  • Goitybell Martínez Téllez Centro de Investigaciones del Ozono
  • Rebeca Hernández Tápanez Centro de Investigaciones del Ozono
  • Maritza Díaz Gómez Centro de Investigaciones del Ozono

DOI:

https://doi.org/10.3989/gya.2005.v56.i2.123

Keywords:

HPT, OFX, Ozonazed sunflower oil, Xylenol orange

Abstract


Total hydroperoxides (HPT) concentration using the ferrous oxidation in xylenol orange (OFX) assay and peroxide value (IP) using iodometric assay, were determined in sunflower oil samples ozonated at different ozone dosages. The effect of an increasing amount of ozonazed sunflower oil was evaluated by assembly assay, where a lineal relationship was obtained between oil amounts from 2 to 17 µg in the assay and absorbance units measured at 560 nm. The OFX reagent calibration and the extinction coefficient calculation were carried out for HPT measurement in ozonazed samples. The correlation established between IP and the concentration obtained by OFX assay showed a lineal relationship (r = 99.29; r2 = 98.59 %). These results confirm that this assay is appropriate for HPT measurement in ozonazed sunflower oil.

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Published

2005-06-30

How to Cite

1.
Martínez Téllez G, Hernández Tápanez R, Díaz Gómez M. Measurement of the total hydroperoxides in ozonated sunflower oil using the ferrous oxidation in xylenol orange assay. grasasaceites [Internet]. 2005Jun.30 [cited 2021Dec.9];56(2):147-52. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/123

Issue

Section

Research

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