Antioxidant and antimicrobial activity in the seeds of Origanum vulgare L. subsp. gracile (C. Koch) Ietswaart and Origanum acutidens (Hand.-Mazz.) Ietswaart from Turkey


  • M. Kurşat Bitlis Eren University, Faculty of Sciences and Arts, Department of Biology, Bitlis
  • İ. Emre Firat University, Faculty of Education, Department of Primary Education, Elazig
  • Ö. Yılmaz Firat University, Faculty of Sciences, Department of Biology, Elazig
  • P. Erecevit Firat University, Faculty of Sciences, Department of Biology, Elazig



Antimicrobial capacity, DPPH radical scavenging activity, Fatty acids, Flavonoids, Origanum L., Sterols, Vitamins


The present study determined some biological compounds (fatty acids, vitamins, sterols and flavonoids), radical scavenging capacity and antimicrobial activity of two Origanum L. species of Origanum vulgare L. subsp. gracile (C. Koch) Ietswaart and Origanum acutidens (Hand.-Mazz.). Ietswaart. Linolenic acid was found to be the main fatty acid in both species, which was followed by palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1 n9), linoleic acid (C18:2 n6) and stearidonic acid (C18:4). In addition, the two Origanum species were recorded as containing high levels of stigmasterol. It was also found that Origanum vulgare L. subsp. gracile (C. Koch) Ietswaart had a high β-sitosterol content (152.8±2.6 µg/g); high amounts of D3 (33.1±0.8 µg/g), K1 (29.4±0.8 µg/g), K2 (26.0±0.8 µg/g) vitamins; and low amounts of α-tocopherol (7.8±0.2 µg/g) and D2 (1.8±0.1 µg/g), while Origanum acutidens (Hand.-Mazz.) Ietswaart contained high amounts of D2 (42.9±1.5 µg/g) vitamin; and low amounts of D3 (2.9±0.1 µg/g), α-tocopherol (3.1±0.1 µg/g), r-tocopherol (3.5±0.2 µg/g), K2 (1.3±0.1 µg/g), retinol (1.3±0.2 µg/g) and retinol acetate (1.2±0.1 µg/g) vitamins. The present study showed that Origanum vulgare L. subsp. gracile (C. Koch) Ietswaart and Origanum acutidens (Hand.-Mazz.) Ietswaart contained the lowest amount of flavonoid. Furthermore, methanol extracts were recorded to be the most effective agent against the DPPH radical in both Origanum L. species examined. Experimental results showed that the vitamins, flavonoids and fatty acid extracts of both Origanum L. species were effective in the inhibition of the growth of the tested microorganisms at varying levels.


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Azcan N, Kara M, Demirci B, Ba_er KHC. 2004. Fatty acids of the seeds of O. onites L. and O. vulgare L. Lipids 39 (5), 487-489. PMid:15506245

Capecka E, Mareczek A, Lejas M. 2005. Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chemistry 93, 223-226.

Cetingul IS, Bayram I, Akkaya AB, Uyarlar C, Yardımcı M, _ahin EH, _engör E. 2007. Utilisation of oregano (Origanum vulgaris) in laying quails (Coturnix coturnix japonica) (2): The effects of oregano on performance, carcass yield, liver and someblood parameters. Archiva Zootechnica 10, 57-65.

Chatzopoulou A, Karioti A, Chrysoula G, Vivancos VL, Kyriazopoulos P, Golegou S, Skaltsa H. 2010. Depsides and other polar constituents from Origanum dictamnus L. and their in vitro antimicrobial activity in clinical strains. J. Agric. Food Chem. 58, 6064-6068 PMid:20423097

Chun SS, Vattem DA, Lin YT, Shetty K. 2005. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry 40, 809-816.

Christie WW. 1990. Gas Chromatography and lipids. The oily press: Glaskow: UK 573-577.

Collins CH, Lyne PM. 1987. Microbiological Methods Butter Morths & Co (Publishers) Ltd. London, pp. 450.

Conforti, F, Sosa S, Marrelli M, Menichini F, Statti GA, Uzunov D, Tubaro A, Menichini F. 2009. The protective ability of Mediterranean dietary plants against the oxidative damage: The role of radical oxygen species in inflammation and the polyphenol, flavonoid and sterol content. Food Chemistry 112, 587-594.

Davis PH, Mill RR, Tan K. 1982. Flora of Turkey and the East Aegean Islands, Edinburgh University Press, vol 7, Edinburgh, 297-313.

Demo A, Petrakis C, Kefales P, Boskou D. 1998. Nutrient antioxidants in some herbs and Mediterrranean plant leaves. Food Research International 31 (5), 351-354.

Dib MA, Bendahou M, Bandiabdellah A, Djabou N, Allali A, Tabti B, Paolini J, Costa J. 2010. Partial chemical composition and antimicrobial activty of Daucus crinitus Desf. extracts. Grasas y Aceites 61, 271-278.

Dikbas N, Kotan R, Dadasoglu F, Karagöz K, Çakmakçı R. 2009. Correlation between major constitiuents and antibacterial activities of some plant essential oils against some pathogenic bacteria. Turkish Journal of Science & Technology 4 (1), 57-64.

Djeridane A, Yous M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. 2006. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chemistry 97, 654-660.

Hara A, Radin NS. 1978. Lipid extraction of tissues with a low-toxicity solvent. Anal. Biochem. 90 (1), 420-426.

Hernández-Hernández E, Ponce-Alquicira E, Jaramillo-Flores ME, Legarreta IG. 2009. Antioxidant effect rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgare L.) extracts on TBARS and colour of model raw pork batters. Meat Science 81, 410-417.

Ivanova D, Gerova D, Chervenkov T, Yankova T. 2005. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. Journal of Ethnopharmacology 96, 145-150. PMid:15588663

Kordalı S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E. 2008. Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresource Technology 99, 8788-8795. PMid:18513954

Kouri G, Tsimogiannis D, Bardouki H, Oreopoulou V. 2007. Extraction and analysis of antioxidant components from Origanum dictamnus. Innovative Food Science and Emerging Technologies 8, 155-162.

Kukiç J, Petrovic S, Niketic M. 2006. Antioxidant activity of four endemic Stachys taxa. Biol. Pharm. Bull. 29 (4), 725-729.

Kulisica T, Radonic A, Katalinic V, Milos M. 2004. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry 85, 633-640.

Kur_at M, Ercevit P. 2009. The Antimicrobial activites of methanolic extracts of some Lamiaceae members collected from Turkey. Turkish Journal of Science & Technology 4, 81-85.

López-Cervantes J, Sánchez-Machado DI, Ríos-Vázquez NJ. 2006. High-performance liquid chromatography method for the simultaneous quantification of retinol, _-tocopherol, and cholesterol in shrimp waste hydrolysate. Journal of Chromatography A 1105, 135-139. PMid:16439259

Liyana-Pathiranan CM, Shahidi F. 2005. Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. J. Agric. Food Chem. 53, 3-2440. PMid:15796575

Logouri V, Boskou D. 1996. Nutrient antioxidants in oregano. International J. Food Science 47, 439-497.

Marin PD, Sajdl V, Kapor S, Tatic B, Petkovic B. 1991. Fatty acids of Saturejoideae, Ajugodeae and Scutellariodeae (Lamiaceae). Phytochemistry 30, 2979-2982.

Milos M, Mastelic J, Jerkovic I. 2000. Chemical composition and antioxidant e.ect of glycosidically bound volatile compounds from oregano (Origanum vulgare L. ssp. hirtum). Food Chem. 71, 79-83.

Mohamed AA, Khali AA, El-Beltagi HES. 2010. Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica). Grasas y Aceites 61, 65-70.

Nakiboglu M, Urek RO, Kayali HA, Tarhan L. 2007. Antioxidant capacities of endemic Sideritis sipylea and Origanum sipyleum from Turkey. Food Chemistry 104, 630-635.

Oliveira JLTM, Diniz MFM, Lima EO, Souza EL, Trajano VN, Santos BHC. 2009. Effectiveness of Origanum vulgare L. and Origanum majorana L. Essential oils in inhibiting the growth of bacterial strains isolated from the patients with conjunctivitis. Brazilian Archives of Biology and Technology 52, 45-50.

Proestos C, Boziaris IS, Nychas G-JE, Komaitis M. 2006. Analysis of flavonoids and phenolic acids in Grek aromatic plants: Investigation of their antioxidant capacity and antimicrobial activity. Food Chemistry 95, 664-671.

Proestos C, Komaitis M. 2008. Application of microwave-assisted extraction to the fast extraction of plant phenolic compounds. LWT 41, 652-659.

Sánchez-Machado DI, López-Hernandez J, Paseiro-Losado P. 2002. High-performance liquid chromatographic determination of ±-tocopherol in macroalgae. J. Chromatography A 976, 277-284. PMid:12462619

Sellami IH, Maamouri E, Chahed T, Aidi-Wannes W, Kchouk ME, Marzouk B. 2009. Effect of growth stage on the content and composition of the essential oil and phenolic fraction of sweet marjoram (Origanum majorana L.) Industrial Crops and Products 30, 395-402.

Skerget M, Kotnik P, Hadolin M, Hras AR, Simonic M, Knez Z. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem. 89, 191-198.

Skoula M, Harborne JB. 2002. The taxonomy and chemistry of Origanum. In: Kintzios (Eed.), S.E. (Ed.), Oregano, the genera Origanum and Lippia -Medicinal and Aromatic Plants Series - Industrial Profiles, vol. 25. Taylor & Francis, London, pp. 67-108.

Skoula M, Grayer RJ, Kite GC, Veitch NC. 2008. Exudate flavones and flavanones in Origanum species and their interspecific variation. Biochemical Systematics and Ecology 36, 646-654.

Ulukapı K, Demiral S, Onus AN, Ülger S. 2008. Bazı Origanum türlerinde dı_arıdan GA3 uygulamalarının in vivo ve in vitro ko_ullarda çimlenme üzerine etkilerinin ara_tırılması. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi 21 (1), 123-129.

Ünver A, Arslan D, Özcan MM, Akbulut M. 2009. Phenolic content and Antioxidant activity of some spices. World Applied Sciences Journal 6 (3), 373-377.

Verma AR, Vijayakumar M, Mathela CS, Rao CV. 2009. In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. Food and Chemical Toxicology 47, 2196-2201. PMid:19520138

Vivacons M, Moreno JJ. 2005. Beta-sitosterol modulates antioxidant enzyme response in RAW 264.7 macrophages. Free Radical Biology & Medicine 39, 91-97. PMid:15925281

Wojdylo A, Oszmianski J, Czemerys R. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 105, 940-949.

Yi_it D, Kandemir A. 2002. Antimicrobial activity of some endemic plants (Salvia cryptantha, Origanum acutidens, Thymus sipyleus ssp. sipyleus). Erzincan E_itim Fakültesi Dergisi, 4-2.

Yoshida Y, Niki E. 2003. Antioxidant effects of phytosterol and its components. Journal of Nutritional Science and Vitaminology 49, 277-280.

Zu YG, Li CY, Fu YJ, Zhao CJ. 2006. Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD. J. Pharmaceutical and Biomedical Analysis 41, 714-719. PMid:16520013




How to Cite

Kurşat M, Emre İ., Yılmaz Ö., Erecevit P. Antioxidant and antimicrobial activity in the seeds of Origanum vulgare L. subsp. gracile (C. Koch) Ietswaart and Origanum acutidens (Hand.-Mazz.) Ietswaart from Turkey. grasasaceites [Internet]. 2011Dec.30 [cited 2022Dec.7];62(4):410-7. Available from:




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