Prospects of fatty acid profile and bioactive composition from lipid seeds for the discrimination of apple varieties with the application of chemometrics


  • S. Arain National Centre of Excellence in Analytical Chemistry, University of Sindh
  • S. T.H. Sherazi National Centre of Excellence in Analytical Chemistry, University of Sindh
  • M. I. Bhanger National Centre of Excellence in Analytical Chemistry, University of Sindh
  • N. Memon National Centre of Excellence in Analytical Chemistry, University of Sindh
  • S. A. Mahesar National Centre of Excellence in Analytical Chemistry, University of Sindh
  • M. T. Rajput Institute of Plant Sciences, University of Sindh



Apple seed varieties, Chemometrics, Crude oil extraction, Fatty acid composition, Lipid bioactives


The extracted oils from four apple seed varieties (Royal Gala, Red Delicious, Pyrus Malus and Golden Delicious) from Pakistan were investigated for their fatty acid profiles and lipid biactives by GC-MS. The oil contents in the seeds of the apple varieties ranged from 26.8-28.7%. The results revealed that linoleic acid (40.5-49.6%) was the main fatty acid in the Royal Gala, Red Delicious and Pyrus Malus seeds, and oleic acid (38.7-45.5%) was the main fatty acid in the Golden Delicious seeds. Palmitic acid (6.1-7.4%) and stearic acid (2.0-3.1%) were the dominant saturated fatty acids, besides the small amount of palmitoleic, heptadecanoic, linolenic, archidic, eicosanoic, and behenicacids. Sterols, tocopherols, hydrocarbons and some other minor components were also identified from the unsaponifiable lipid fraction. The variation among the results of both fatty acids and lipid bioactives for the four different varieties was assessed by principal component analysis, discriminant analysis and cluster analyses. The results conclude that both oil fractions could be applied as a useful tool to discriminate among the apple seed varieties.


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How to Cite

Arain S, Sherazi ST, Bhanger MI, Memon N, Mahesar SA, Rajput MT. Prospects of fatty acid profile and bioactive composition from lipid seeds for the discrimination of apple varieties with the application of chemometrics. grasasaceites [Internet]. 2012Jun.30 [cited 2022Dec.7];63(2):175-83. Available from:




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