An AS-PCR assay for accurate genotyping of <i>FAD2A/FAD2B</i> genes in peanuts (<i>Arachis hypogaea</i> L.)

H. T. Yu; W. Q. Yang; Y. Y. Tang; X. Z. Wang; Q. Wu; D. Q. Hu; C. T. Wang; S. L. Yu

doi:10.3989/gya.118712

An AS-PCR assay for accurate genotyping of FAD2A/FAD2B genes in peanuts (Arachis hypogaea L.)

Authors

H. T. Yu Shandong Peanut Research Institute
W. Q. Yang Shandong Peanut Research Institute
Y. Y. Tang Shandong Peanut Research Institute
X. Z. Wang Shandong Peanut Research Institute
Q. Wu Shandong Peanut Research Institute
D. Q. Hu Qingdao Entry-Exit Inspection and Quarantine Bureau
C. T. Wang Shandong Peanut Research Institute
S. L. Yu Shandong Peanut Research Institute

DOI:

https://doi.org/10.3989/gya.118712

Keywords:

Allele specific PCR (AS-PCR), FAD2A/FAD2B, Genotyping, Peanut

Abstract

FAD2A and FAD2B are homoeologous genes from A and B genomes in cultivated peanuts (Arachis hypogaea L.) encoding fatty acid desaturates which convert oleate to linoleate. To study the genetics of oleate and breed high oleate peanut cultivars, a simple allele specific-PCR (AS-PCR) protocol for the accurate genotyping of FAD2A/FAD2B was developed to discriminate the wild and mutant allele of both genes (FAD2A 448 G > A and FAD2B 441_442insA). The results may serve to develop a feasible procedure for producing highly desired high oleate peanut cultivars through hybridization.

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Published

2013-09-30

How to Cite

Yu HT, Yang WQ, Tang YY, Wang XZ, Wu Q, Hu DQ, Wang CT, Yu SL. An AS-PCR assay for accurate genotyping of FAD2A/FAD2B genes in peanuts (Arachis hypogaea L.). Grasas aceites [Internet]. 2013Sep.30 [cited 2025May2];64(4):395-9. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1445

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Issue

Vol. 64 No. 4 (2013)

Section

Research

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