An AS-PCR assay for accurate genotyping of FAD2A/FAD2B genes in peanuts (Arachis hypogaea L.)
DOI:
https://doi.org/10.3989/gya.118712Keywords:
Allele specific PCR (AS-PCR), FAD2A/FAD2B, Genotyping, PeanutAbstract
FAD2A and FAD2B are homoeologous genes from A and B genomes in cultivated peanuts (Arachis hypogaea L.) encoding fatty acid desaturates which convert oleate to linoleate. To study the genetics of oleate and breed high oleate peanut cultivars, a simple allele specific-PCR (AS-PCR) protocol for the accurate genotyping of FAD2A/FAD2B was developed to discriminate the wild and mutant allele of both genes (FAD2A 448 G > A and FAD2B 441_442insA). The results may serve to develop a feasible procedure for producing highly desired high oleate peanut cultivars through hybridization.
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Published
2013-09-30
How to Cite
1.
Yu HT, Yang WQ, Tang YY, Wang XZ, Wu Q, Hu DQ, Wang CT, Yu SL. An AS-PCR assay for accurate genotyping of FAD2A/FAD2B genes in peanuts (Arachis hypogaea L.). Grasas aceites [Internet]. 2013Sep.30 [cited 2024Apr.26];64(4):395-9. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1445
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Research
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