Evaluation of diffusional effects on kinetics of soybean lecithin hydrolysis using A2 phospholipase

Authors

  • B. Maroto Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba
  • C. Camusso Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba
  • N. Zaritzky Facultad de Ingeniería UNLP y CIDCA, Facultad de Ciencias Exactas, Universidad Nacional de La Plata

DOI:

https://doi.org/10.3989/gya.2004.v55.i2.160

Keywords:

Diffusional effects, Effectiveness factor, Immobilized A2 phospholipase, Mass transport, Soybean lecithin

Abstract


Soluble or immobilized A2 phospholipase liberates one mole of fatty acid of the C-2 position in soybean lecithin phospholipids to product lysolecithins. When using the enzyme in its immobilized form the enzymatic activity decreases due to substrate and product transport phenomenon. By comparison of the kinetic parameters of the hydrolysis reaction catalized by A2 phospholipase immobilized on alumina or on DEAE-Sephadex against those of the soluble enzyme, for soybean lecithin hydrolysis reaction, the diffusive effects were evaluated. The decrease of the reaction rate was quantified in presence of diffusive effects and the effectiveness factors were calculated. The Michaelis-Menten kinetic parameters were determined: KM = 8,2.10-2 mol·l-1 and 5,36.10-2 mol·l-1 and Vmax = 7,7.10-5 mol·l-1 .min-1 and 9,5.10-5 mol·l-1 ·min-1 for the enzyme immobilized on alumina and on DEAE-Sephadex, respectively. The effectiveness factors were 0,277 for alumina and 0,376 for DEAE-Sephadex.

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Published

2004-06-30

How to Cite

1.
Maroto B, Camusso C, Zaritzky N. Evaluation of diffusional effects on kinetics of soybean lecithin hydrolysis using A2 phospholipase. grasasaceites [Internet]. 2004Jun.30 [cited 2022Dec.1];55(2):148-54. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/160

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Section

Research