n-3 LCPUFA in the reversal of hepatic steatosis: the role of ACOX and CAT-1


  • G. S. Tapia Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile
  • D. González-Mañán Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile
  • A. D’Espessailles Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile
  • C. G. Dossi Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile




ACOX (Acyl coenzyme A oxidase), AP-1(activating protein 1), CAT-1 (carnitine acyl transferase I), HFD (high fat diet), NAFLD (non-alcoholic fatty liver disease), n-3 LCPUFA (n-3 long chain polyunsaturated fatty acid), Reversion


The aim of this study was to investigate the roles of the Acyl co-enzyme A oxidase (ACOX), carnitine acyl transferase I (CAT-1) and activating protein 1 (AP-1) in the reversal of hepatic steatosis with dietary change and n-3 long chain polyunsaturated fatty acid (n-3 LCPUFA) supplementation. Male C57BL/6J mice were given either a control diet (CD) or a high fat diet (HFD) for 12 weeks, and then continued with the CD or CD plus n-3 LCPUFA for eight weeks. After this period, body and adipose visceral tissue weight were analyzed and liver samples were taken to measure ACOX, CAT-1 and c-jun levels. The dietary change from HFD to a norm caloric diet plus n-3 LCPUFA supplementation significantly reduced liver steatosis and adipose tissue: body weight ratio, along with an increase in the hepatic ACOX and CAT-1 levels and normalization of AP-1 expression that could favor the fatty acid beta-oxidation over lipogenesis and regulate inflammation. These results provide new data on the enzymatic metabolism underlying dietary change to a norm caloric diet plus n-3 LCPUFA supplementation.


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How to Cite

Tapia GS, González-Mañán D, D’Espessailles A, Dossi CG. n-3 LCPUFA in the reversal of hepatic steatosis: the role of ACOX and CAT-1. grasasaceites [Internet]. 2016Jun.30 [cited 2022Dec.4];67(2):e134. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1600