Enrichment of Cinta Senese burgers with omega-3 fatty acids. Effect of type of addition and storage conditions on quality characteristics





Fish oil, Meat quality, Microencapsulation, Pork, Sensorial attributes


The most beneficial omega-3 PUFAs to human health, EPA and DHA fatty acids, are typically present in fish products, but extraneous to meat. Therefore, Cinta Senese pork burgers were added with microencapsulated (M) and bulk fish oil (F) and subjected to three storage conditions: no storage (T0), chilled (T5) and frozen storage (T30). The physico-chemical and sensory attributes of raw and cooked burgers were investigated. After storage and cooking, EPA and DHA were better preserved in M burgers than in F samples, which showed the highest TBAR values at T0 and T5, while M samples presented scores similar to the control. Panelists observed differences mainly in greasy appearance, odor intensity and cooked meat odor and flavor. The M group showed the best scores at T5 with respect to the control and F burgers. So, fish oil microencapsulation was an effective method to prevent EPA and DHA oxidation while respecting burger quality characteristics.


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A.O.A.C. 2000a. Moisture in Malt Gravimetric Method. (935.29). In Official Methods of Analysis (17th ed.). Gaithersburg, Maryland: Association of Official Analytical Chemist.

A.O.A.C. 2000b. Crude Protein in Meat: Block Digestion Method. (992.15). In Official Methods of Analysis (17th ed.). Gaithersburg, Maryland: Association of Official Analytical Chemist.

A.O.A.C. 2000c. Methyl Esters of Fatty Acids in Oils and Fats: Gas Chromatographic Method. (963.22). In Official Methods of Analysis (17th ed.). Gaithersburg, Maryland: Association of Official Analytical Chemist.

Baggio SR, Bragagnolo, N. 2006. The effect of heat treatment on the cholesterol oxides, cholesterol, total lipid and fatty acid contents of processed meat products. Food Chem. 95, 611–619. https://doi.org/10.1016/j.foodchem.2005.01.037

Cáceres E, García ML, Selgas MD. 2008. Effect of pre-emulsified fish oil - as source of PUFA n-3 - on microstructure and sensory properties of mortadella, a Spanish bologna-type sausage. Meat Sci. 80, 183–193. https://doi.org/10.1016/j.meatsci.2007.11.018 PMid:22063321

Corino C, Rossi R, Cannata S, Ratti S. 2014. Effect of dietary linseed on the nutritional value and quality of pork and pork products: Systematic review and meta-analysis. Meat Sci. 98. https://doi.org/10.1016/j.meatsci.2014.06.041

Dienke BJ, Van Montfort SJT, Oranje B, Durston S, Smeets PAM. 2016. Effects of omega-3 polyunsaturated fatty acids on human brain morphology and function: what is the evidence?. Eur. Neuropsychopharmacol. 26, 546–61. https://doi.org/10.1016/j.euroneuro.2015.12.031 PMid:26742901

García-Almeida JM, Murri-Pierri M, Lupié-ez Y, Rico-Pérez JM, Saracho-Dominguez H, Roca MM, Garcia-Aleman J, Casado-Fernéndez G, Medina-Carmona J, Tinahones- Madue-o F. 2010. PP299 effect of oral supplementation enriched with omega-3 fatty acids in inflammatory parameters and oxidative stress in patients with otolaryngologist cancer treated with radiotherapy. Clin. Nutr. Supplements 5.

Garg ML, Wood LG, Singh H, Moughan PJ. 2006. Means of delivering recommended levels of long chain n-3 polyunsaturated fatty acids in human diets. J. Food Sci. 71. https://doi.org/10.1111/j.1750-3841.2006.00033.x

Givens DI, Kliem KE, Gibbs RA. 2006. The role of meat as a source of n-3 polyunsaturated fatty acids in the human diet. Meat Sci. 74, 209–18. https://doi.org/10.1016/j.meatsci.2006.04.008 PMid:22062730

Greene BE, Cumuze TH. 1982. Relationship Between TBA Numbers and Inexperienced Panelists'Assessments of Oxidized Flavor in Cooked Beef. J. Food Sci. 47, 52–54. https://doi.org/10.1111/j.1365-2621.1982.tb11025.x

Heck RT, Vendruscolo RG, de Araújo Etchepare M, Cichoski AJ, de Menezes CR, Barin JS, Campagnol PCB. 2017. Is it possible to produce a low-fat burger with a healthy n ? 6/n ? 3 PUFA ratio without affecting the technological and sensory properties? Meat Sci. 130, 16–25. https://doi.org/10.1016/j.meatsci.2017.03.010 PMid:28347883

Jiménez-Colmenero F. 2007. Healthier lipid formulation approaches in meat-based functional foods. Technological options for replacement of meat fats by non-meat fats. Trends Food Sci. Technol. 18, 567–578. https://doi.org/10.1016/j.tifs.2007.05.006

Jiménez-Martín E, Gharsallaoui A, Pérez-Palacios T, Carrascal JR, Rojas TA. 2014. Suitability of Using Monolayered and Multilayered Emulsions for Microencapsulation of ?-3 Fatty Acids by Spray Drying: Effect of Storage at Different Temperatures. Food Bioprocess Technol. 8, 100–111. https://doi.org/10.1007/s11947-014-1382-y

Jiménez-Martín E, Pérez-Palacios T, Carrascal JR, Rojas TA. 2016a. Enrichment of Chicken Nuggets with Microencapsulated Omega-3 Fish Oil: Effect of Frozen Storage Time on Oxidative Stability and Sensory Quality. Food Bioprocess Technol. 9, 285–297. https://doi.org/10.1007/s11947-015-1621-x

Jiménez-Martín E, Rojas TA, Gharsallaoui A, Carrascal JR, Pérez-Palacios T. 2016b. Fatty acid composition in double and multilayered microcapsules of ?-3 as affected by storage conditions and type of emulsions. Food Chem. 194, 476–486. https://doi.org/10.1016/j.foodchem.2015.08.046 PMid:26471582

Josquin NM, Linsse JPH, Houben JH. 2012. Quality characteristics of Dutch-style fermented sausages manufactured with partial replacement of pork back-fat with pure, pre-emulsified or encapsulated fish oil. Meat Sci. 90, 81–6. https://doi.org/10.1016/j.meatsci.2011.06.001 PMid:21703777

Keenan DF, Resconi VC, Smyth TJ, Botinestean C, Lefranc C, Kerry JP, Hamill RM. 2015. The effect of partial-fat substitutions with encapsulated and unencapsulated fish oils on the technological and eating quality of beef burgers over storage. Meat Sci. 107, 75–85. https://doi.org/10.1016/j.meatsci.2015.04.013 PMid:25965966

Martínez B, Miranda JM, Vázquez BI, Fente CA, Franco CM, Rodríguez JL, Cepeda A. 2012. Development of a Hamburger Patty with Healthier Lipid Formulation and Study of its Nutritional, Sensory, and Stability Properties. Food Bioprocess Technol. 5, 200–208. https://doi.org/10.1007/s11947-009-0268-x

Pelliccia F, Marazzi G, Greco C, Franzoni F, Speziale G, Gaudio C. 2013. Current evidence and future perspectives on n-3 PUFAs. Int. J. Cardiol. 170, S3–S7. https://doi.org/10.1016/j.ijcard.2013.06.044 PMid:23871631

Pelser WM, Linssen JPH, Legger A, Houben JH. 2007. Lipid oxidation in n - 3 fatty acid enriched Dutch style fermented sausages. Meat Sci. 75, 1-11. https://doi.org/10.1016/j.meatsci.2006.06.007 PMid:22063405

Pérez-Palacios T, Ruiz J, Martín D, Muriel E, Antequera T. 2008. Comparison of different methods for total lipid quantification in meat and meat products. Food Chem. 110, 1025–1029. https://doi.org/10.1016/j.foodchem.2008.03.026 PMid:26047297

Pugliese C, Sirtori F. 2012. Quality of meat and meat products produced from southern European pig breeds. Meat Sci. 90, 511–8. https://doi.org/10.1016/j.meatsci.2011.09.019 PMid:22030111

Pugliese C, Bozzi R, Campodoni G, Acciaioli A, Franci O, Gandini G. 2005. Performance of Cinta Senese pigs reared outdoors and indoors. 1. Meat and subcutaneous fat characteristics. Meat Sci. 69, 459–64. https://doi.org/10.1016/j.meatsci.2004.09.001 PMid:22062984

Salih AM, Smith DM, Price JF, Dawson LE. 1987. Modified extraction 2-thiobarbituric acid method for measuring lipid oxidation in poultry. Poul. Sci. 66, 1483–1488. https://doi.org/10.3382/ps.0661483 PMid:3684874

Salminen H, Herrmann K, Weiss J. 2013. Oil-in-water emulsions as a delivery system for n-3 fatty acids in meat products. Meat Sci. 93, 659–667. https://doi.org/10.1016/j.meatsci.2012.11.035 PMid:23273478

Sanders TA. 2000. Polyunsaturated fatty acids in the food chain in Europe. Am. J. Clin. Nutr. 71, 176S-8S. https://doi.org/10.1093/ajcn/71.1.176s PMid:10617968

SAS. (1996). SAS/STAT software, release 9.4. SAS Institute Inc., Cary, NC.

Valencia I, O'Grady MN, Ansorena D, Astiasarán I, Kerry JP. 2008. Enhancement of the nutritional status and quality of fresh pork sausages following the addition of linseed oil, fish oil and natural antioxidants. Meat Sci. 80, 1046–54. https://doi.org/10.1016/j.meatsci.2008.04.024 PMid:22063835

Wood JD, Enser M, Fisher A V., Nute GR, Sheard PR, Richardson RI, Hughes SI, Whittington FM. 2008. Fat deposition, fatty acid composition and meat quality: A review. Meat Sci. 78, 343-358. https://doi.org/10.1016/j.meatsci.2007.07.019 PMid:22062452



How to Cite

Aquilani C, Pérez-Palacios T, Sirtori F, Jiménez-Martín E, Antequera T, Franci O, Acciaioli A, Bozzi R, Pugliese C. Enrichment of Cinta Senese burgers with omega-3 fatty acids. Effect of type of addition and storage conditions on quality characteristics. grasasaceites [Internet]. 2018Mar.30 [cited 2021Jul.24];69(1):e235. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1702