Enzyme extraction of cupuassu (Theobroma grandiflorum S.) fat sedes


  • D.C.S. da Silva Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos (PPGCTA), Instituto de Tecnologia (ITEC), Universidade Federal do Pará (UFPA) https://orcid.org/0000-0003-0424-1613
  • A.M.C. Rodrigues Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos (PPGCTA), Instituto de Tecnologia (ITEC), Universidade Federal do Pará (UFPA) https://orcid.org/0000-0003-1268-5652
  • L.H.M. da Silva Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos (PPGCTA), Instituto de Tecnologia (ITEC), Universidade Federal do Pará (UFPA) https://orcid.org/0000-0002-6002-9425




Amazon seeds, By-products properties, Green extraction, Theobroma grandiflorum


Enzyme-assisted extraction is considered an environmentally friendly technique. Cellulase, pectinase and protease were tested for cupuassu seeds fat extraction. The best fat efficiency (81.66%) was obtained for the solute:solvent 1:5 (m:w), orbital shaker at 120 rpm, 60 °C, for 8 hours and enzyme concentrations (cellulase, pectinase and protease) of 1.0%. The fat was characterized for physicochemical properties, fatty acid profile, phenolic compounds, antioxidant activities and oxidative stability. The fat showed good thermal stability (14.26 h) and high contents of monounsaturated (42.42%) and saturated (43.47%) fatty acids with higher concentrations of oleic and stearic acids, respectively, and a high content of phenolic compounds (141.84 µg EAG·g-1) in the fat, and in the aqueous extract (926.47 µg EAG·g-1). The results indicated that the cupuassu seed fat obtained by enzymatic extraction showed superior properties to cupuassu fat obtained by cold pressing, in addition to generating an aqueous fraction which is rich in bioactive compounds that can be used as ingredients in the food and pharmaceutical sectors.


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How to Cite

da Silva D, Rodrigues A, da Silva L. Enzyme extraction of cupuassu (Theobroma grandiflorum S.) fat sedes. grasasaceites [Internet]. 2023May25 [cited 2023May28];74(2):e498. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1978

Funding data

Conselho Nacional de Desenvolvimento Científico e Tecnológico
Grant numbers 309876/2016-8;308396/2018-9;313453/2019-5

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