Modulation of essential fatty acid levels in coconut oil with flaxseed oil




Blending, Coconut oil, Essential fatty acids, Flaxseed oil, ω-3 fatty acids, ω-6:ω-3 ratio


Coconut oil (CO) is a popular cooking medium but its lack of essential fatty acids (FA) is a health concern. Therefore, the aim of this work was to improve the FA profile of CO by blending with flaxseed oil (FO). Blends with various percentages of FO were prepared and studied for physicochemical characterization, thermal and long-term storage stability. The results indicated that the blends made favorable alterations in FA composition without adverse effects to the oxidative stability of the fatty acids and they resisted secondary thermal deterioration up to two hours at 180 ºC. The blend with the highest percentage of FO was stable for nine months. THP-1 cell line studies showed that ω-3 FA from the blend was incorporated into the cells with no adverse effect on cell viability but the inflammatory markers studied remained unaltered. Thus, CO and FO blends could be stored for at least nine months and could be used as cooking medium when prolonged heating is not involved.


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Bhardwaj K, Verma N, Trivedi RK, Bhardwaj S, Shukla N. 2015. A novel approach for improvement of oxidative stability of flaxseed oil by blending with palm oil. Int. J. Adv. Res. 3, 1399-1407.

Bhatnagar AS, Prasanth Kumar PK, Hemavathy J, Gopala Krishna AG. 2009. Fatty acid composition, oxidative stability, and radical scavenging activity of vegetable oil blends with coconut oil. J. Am. Oil Chem. Soc. 86, 991-999.

Chandrashekar P, Lokesh BR, Krishna AG. 2010. Hypolipidemic effect of blends of coconut oil with soybean oil or sunflower oil in experimental rats. Food Chem. 123, 728-733.

CODEX. 1999. Codex Alimentarius Commission: Fats, Oils & Related Products By Codex Alimentarius Commission-Codex Standard for Edible Fats and Oils not Covered by Individual Standards (CODEX STAN 19-1981, Rev. 2-1999)

Das AK, Babylatha R, Pavithra AS, Khatoon S. 2013. Thermal degradation of groundnut oil during continuous and intermittent frying. J. Food Sci. Technol. 50, 1186-1192.

De Boer AA, Ismail A, Marshall K, Bannenberg G, Yan KL Rowe WJ. 2018. Examination of marine and vegetable oil oxidation data from a multi-year, third-party database. Food Chem. 254, 249-255.

Eyres L, Eyres MF, Chisholm A, Brown RC. 2016. Coconut oil consumption and cardiovascular risk factors in humans. Nutr. Rev. 74, 267-280.

Folch J, Lees M, Stanley GS. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497-509.

Gil-Villarino A, Garcı́a-Fuentes E, Zafra MF, Garcı́a-Peregrı́n E. 1999. Coconut oil induces short-term changes in lipid composition and enzyme activity of chick hepatic mitochondria. J. Nutr. Biochem. 10, 325-330.

Guillaume C, De Alzaa F, Ravetti L. 2018. Evaluation of chemical and physical changes in different commercial oils during heating. Acta Sci. Nutrit. Health 2, 02-11

Hintze KJ, Tawzer J, Ward RE. 2016. Concentration and ratio of essential fatty acids influences the inflammatory response in lipopolysaccharide challenged mice. Prostag. Leukotr. Ess. 111, 37-44.

Ichihara KI, Fukubayashi Y. 2010. Preparation of fatty acid methyl esters for gas-liquid chromatography. J. Lipid Res. 51, 635-640.

Jagtap AA, Badhe YS, Hegde MV, Zanwar AA. 2020. Development and characterization of stabilized omega-3 fatty acid and micronutrient emulsion formulation for food fortification. J. Food Sci. Technol. 58, 996-1004.

Joshi AA, Hegde MV, Zanwar AA. 2022. Flaxseed oil and palm olein blend to improve omega-6:omega-3 ratio. J. Food Sci. Technol. 59, 498-509.

Kaur N, Chugh V, Gupta AK. 2014. Essential fatty acids as functional components of foods-a review. J. Food Sci. Technol. 51, 2289-2303.

Kumar PP, Bhatnagar AS, Hemavathy J, Krishna AG. 2009. Changes in physico-chemical characteristics of some vegetable oils upon blending with coconut oil. J. Lipid Sci. Technol. 41, 136-142.

Kumar PP, Krishna AG. 2015. Physicochemical characteristics of commercial coconut oils produced in India. Grasas Aceites 66 (1), e062.

Lima RD, Block JM. 2019. Coconut oil: what do we really know about it so far?. Food Qual Saf 3, 61-72.

Mahesar SA, Sherazi STH, Khaskheli AR, Kandhro AA. 2014. Analytical approaches for the assessment of free fatty acids in oils and fats. Ana.l Methods 6, 4956-4963.

Malvis A, Šimon P, Dubaj T, Sládková A, Ház A, Jablonsky M, Sekretár S, Schmidt Š, Kreps F, Burčová Z, Hodaifa G. 2019. Determination of the thermal oxidation stability and the kinetic parameters of commercial extra virgin olive oils from different varieties. J. Chem. 2019.

Maurikaa CS, Jaganivash B, Shanmugasundaram S. 2020. Comparative studies on physicochemical properties of virgin coconut oil (VCO) with different coconut oils. Int. J. Chem. Stud. 8, 2433-2438.

Moigradean D, Poiana MA, Gogoasa I. 2012. Quality characteristics and oxidative stability of coconut oil during storage. J. Agroal. Processes Technol. 18, 272-276.

Nering E. 2016. Evaluation of formulations and oxidative stability of coconut oil blends. Rutgers University-Graduate School-New Brunswick

Pazzoti G, Souza C, Veronezi C, Luzia D, Jorge N. 2018. Evaluation of oxidative stability of compound oils under accelerated storage conditions. Braz. Arch. Biol. Technol. 61.

Perera DN, Hewavitharana GG, Navaratne SB. 2020. Determination of Physicochemical and Functional Properties of Coconut Oil by Incorporating Bioactive Compounds in Selected Spices. J. Lipids 2020.

Srivastava Y, Semwal AD, Sajeevkumar VA, Sharma GK. 2017. Melting, crystallization and storage stability of virgin coconut oil and its blends by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR). J. Food Sci. Technol. 54, 45-54.

Subramanian K. 2019. A Comprehensive Study on Thermal Degdradation of Selective Edible Vegetable Oils By Simultaneous Thermogravimetric and Differential Thermal Analyses. J. Pharm. Sc. Res. 11, 3201-3209.

Symoniuk E, Ratusz K, Krygier K. 2016. Comparison of the oxidative stability of linseed (Linum usitatissimum L.) oil by pressure differential scanning calorimetry and Rancimat measurements. J. Food Sci. Technol. 53, 3986-3995.

Tham YY, Choo QC, Muhammad TS, Chew CH. 2020. Lauric acid alleviates insulin resistance by improving mitochondrial biogenesis in THP-1 macrophages. Mol. Biol. Rep. 47, 9595-607.

Varma SR, Sivaprakasam TO, Arumugam I, Dilip N, Raghuraman M, Pavan KB, Rafiq M, Paramesh R. 2019. In vitro anti-inflammatory and skin protective properties of Virgin coconut oil. J. Tradit. Compl. Med. 9, 5-14.

Wallace TC. 2019. Health effects of coconut oil-A narrative review of current evidence. J. Am. Coll. Nutr. 38, 97-107.

Zhao G, Etherton TD, Martin KR, Gillies PJ, West SG, Kris-Etherton PM. 2007. Dietary α-linolenic acid inhibits proinflammatory cytokine production by peripheral blood mononuclear cells in hypercholesterolemic subjects. Am. J. Clin. Nutr. 85, 385-91.



How to Cite

Joshi A, Hegde M, Zanwar A. Modulation of essential fatty acid levels in coconut oil with flaxseed oil. grasasaceites [Internet]. 2023Jun.13 [cited 2023Sep.23];74(2):e503. Available from: