Utilisation of rapeseed protein isolates for production of peptides with angiotensin I-converting enzyme (ACE)-inhibitory activity

Authors

  • Justo Pedroche Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC).
  • María M. Yust Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC).
  • Cristina Megías Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC).
  • Hassane Lqari Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC).
  • Manuel Alaiz Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC).
  • Julio Girón-Calle Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC).
  • Francisco Millán Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC).
  • Javier Vioque Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC).

DOI:

https://doi.org/10.3989/gya.2004.v55.i4.200

Keywords:

Angiotensin converting enzyme, Peptide inhibitors, Protein hydrolysate, Protein isolate, Rapeseed

Abstract


ACE activity is related to increased arterial pressure and coronary diseases. A rapeseed protein isolate was hydrolyzed with the protease Alcalase in order to investigate the possible presence of ACE inhibitory peptides in the resulting hydrolysates. Hydrolysis for 30 min yielded a hydrolysate with the highest ACE inhibitory activity. Two fractions of this hydrolysate obtained by Biogel P2 gel filtration chromatography were used for further purification of ACE inhibitory peptides. Three fractions with ACE inhibitory activity were purified by reverse-phase HPLC of Biogel P2 f ractions. This demonstrates that rapeseed protein hydrolysates represent a good source of ACE inhibitory peptides .

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Published

2004-12-30

How to Cite

1.
Pedroche J, Yust MM, Megías C, Lqari H, Alaiz M, Girón-Calle J, Millán F, Vioque J. Utilisation of rapeseed protein isolates for production of peptides with angiotensin I-converting enzyme (ACE)-inhibitory activity. grasasaceites [Internet]. 2004Dec.30 [cited 2022Dec.7];55(4):354-8. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/200

Issue

Section

Research

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