Phenol content and β-glucosidase activity during the ripening period of olive fruits (Erkence cv.) from different locations

E.  Susamcı; Ö.  Tuncay; H. Bayraktar; S. Önal

doi:10.3989/gya.0865231.2017

Authors

E. Susamcı Olive Research Institute, Üniversite Caddesi https://orcid.org/0000-0003-1956-4634
Ö. Tuncay Faculty of Agriculture, Ege University https://orcid.org/0000-0002-5218-1056
H. Bayraktar Faculty of Science, Biochemistry Department, Ege University - Faculty of Health Sciences, Department of Nutrition and Dietetics, Yuksek Ihtisas University https://orcid.org/0000-0002-2997-5309
S. Önal Faculty of Science, Biochemistry Department, Ege University https://orcid.org/0000-0003-1418-6443

DOI:

https://doi.org/10.3989/gya.0865231.2017

Keywords:

Altitude, climate, enzyme, olive, phenol, β-glucosidase

Abstract

In the present study, Erkence olive fruits were examined during two harvest periods and in two orchards close to the sea at low altitude (A) and far from the sea at higher altitude (B). Olives from the sea-facing northeast (NE) and land-facing southwest (SW) orientations of the trees were analyzed in terms of maturity index, total phenol content, phenolic compounds, β-glucosidase enzyme activity, total protein content and specific enzyme activity. Climate data were collected from the trees and from the meteorology station. The maturity index, β-glucosidase enzyme activity, total protein content and specific enzyme activity of the olives increased during the ripening period. On the other hand, the total phenol content, oleuropein and other phenolic compound contents in the olives decreased. The altitude and location of the fruits on the tree affected the ripening process. Lower altitude and sea-facing side of the tree resulted in an increased enzyme activity, thus aiding in the ripening process.

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References

Aktas AB, Ozen B, Tokatli F, Sen I. 2014. Phenolics profile of a naturally debittering olive in comparison to regular olive varieties. J. Sci. Food Agric. 94, 691-698. https://doi.org/10.1002/jsfa.6308 PMid:23868414

Borges TH, Pereira JA, Cabrera-Vique C, Lara L, Oliveira AF, Seiquer I. 2017. Characterization of Arbequina virgin olive oils produced in different regions of Brazil and Spain: Physicochemical properties, oxidative stability and fatty acid profile. Food Chem. 215, 454-462. https://doi.org/10.1016/j.foodchem.2016.07.162 PMid:27542498

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3 PMid:942051

Di Vaio C, Nocerino S, Paduano A, Sacchi R. 2013. Influence of some environmental factors on drupe maturation and olive oil composition. J. Sci. Food Agric. 93, 1134-1139. https://doi.org/10.1002/jsfa.5863 PMid:22936522

Ebrahimzadeh H, Motamed N, Rastgar-Jazii F, Montasser-Kouhsari S, Shokraii EH. 2003. Oxidative enzyme activities and soluble protein content in leaves and fruits of olives. J. Food Biochem. 27, 181-196. https://doi.org/10.1111/j.1745-4514.2003.tb00276.x

García-Vico L, García-Rodríguez R, Sanz C, Pérez AG. 2017. Biochemical aspects of olive freezing-damage: Impact on the phenolic and volatile profiles of virgin olive oil. LWT-Food Science and Technology, 86, 240-246. https://doi.org/10.1016/j.lwt.2017.07.052

Gómez-Del-Campo M, García JM. 2012. Canopy fruit location can affect olive oil quality in "Arbequina" hedgerow orchards. J. Am. Oil Chem. Soc. 89, 123-133. https://doi.org/10.1007/s11746-011-1900-2

Gutiérrez-Rosales F, Romero MP, Casanovas M, Motilva MJ, Mínguez-Mosquera MI. 2012. β-Glucosidase involvement in the formation and transformation of oleuropein during the growth and development of olive fruits (Olea Europaea L. Cv. Arbequina) grown under different farming practices. J. Agric. Food Chem. 60 (17), 4348-4358. https://doi.org/10.1021/jf205209y PMid:22475562

Hrncirik K, Fritsche S. 2004. Comparability and reliability of different techniques for the determination of phenolic compounds in virgin olive oil. Eur. J. Lipid Sci. Technol. 106, 540-549. https://doi.org/10.1002/ejlt.200400942

Hu Y, Luan H, Hao D, Xiao H, Yang S, Yang L. 2007. Purification and characterization of a novel ginsenoside-hydrolyzing β-D-glucosidase from the China white jade snail (Achatina Fulica). Enzyme Microb. Technol. 40, 1358-1366. https://doi.org/10.1016/j.enzmictec.2006.10.011

Koudounas K, Banilas G, Michaelidis C, Demoliou C, Rigas S, Hatzopoulos P. 2015. A defence-related Olea europaea β-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent. J. Experiment. Bot. 66, 2093-2106. https://doi.org/10.1093/jxb/erv002 PMid:25697790 PMCid:PMC4669557

Lopez-Huertas E, del Río LA. 2014. Characterization of antioxidant enzymes and peroxisomes of olive (Olea europaea L.) fruits. J. Plant Physiol. 171, 1463-1471. https://doi.org/10.1016/j.jplph.2014.06.014 PMid:25105232

Menz G, Vriesekoop F. 2010. Physical and chemical changes during the maturation of gordal sevillana olives (Olea europaea L., cv. Gordal Sevillana). J. Agric. Food Chem. 58, 4934-4938. https://doi.org/10.1021/jf904311r PMid:20355715

Morales LO, Tegelberg R, Brosché M, Keinänen M, Lindfors A, Aphalo PJ. 2010. Effects of solar UV-A and UV-B radiation on gene expression and phenolic accumulation in Betula pendula leaves. Tree Physiol. 30, 923-934. https://doi.org/10.1093/treephys/tpq051 PMid:20519675

Morello JR, Vuorela S, Romero MP, Motilva MJ, Heinonen M. 2005. Antioxidant activity of olive pulp and olive oil phenolic compounds of the Arbequina cultivar. J. Agric. Food Chem. 53, 2002-2008. https://doi.org/10.1021/jf048386a PMid:15769127

Mousa YM, Gerasopoulos D, Metzidakis I, Kiritsakis A. 1996. Effect of altitude on fruit and oil quality characteristics of "Mastoides" olives. J. Sci. Food Agric. 71, 345-350. https://doi.org/10.1002/(SICI)1097-0010(199607)71:3<345::AID-JSFA590>3.3.CO;2-K

Ortega-García F, Blanco S, Peinado MÁ, Peragón J. 2008. Polyphenol oxidase and its relationship with oleuropein concentration in fruits and leaves of olive (Olea europaea) cv. "Picual" trees during fruit ripening. Tree Physiol. 28, 45-54. https://doi.org/10.1093/treephys/28.1.45 PMid:17938113

Padilla MN, Hernández ML, Sanz C, Martínez-Rivas JM. 2014. Stress-dependent regulation of 13-lipoxygenases and 13-hydroperoxide lyase in olive fruit mesocarp. Phytochem. 102, 80-88. https://doi.org/10.1016/j.phytochem.2014.01.024 PMid:24629805

Parkin KL, Marangoni, A, Jackman RL, Yada RY, Stanley DW. 1989. Chilling Injury, A Review of Possible Mechanisms. J. Food Biochem. 13(2), 127-153. https://doi.org/10.1111/j.1745-4514.1989.tb00389.x

Rallo L, Díez CM, Morales-Sillero A, Miho H, Priego-Capotec F, Rallo P. 2018. Quality of olives: A focus on agricultural preharvest factors. Sci. Horticult. 233, 491-509. https://doi.org/10.1016/j.scienta.2017.12.034

Ramírez E, Gandul-Rojas B, Romero C, Brenes M, Gallardo-Guerrero L. 2015. Composition of pigments and colour changes in green table olives related to processing type. Food Chem. 166, 115-124. https://doi.org/10.1016/j.foodchem.2014.05.154 PMid:25053036

Ramírez E, Brenes M, García P, Medina E, Romero C. 2016. Oleuropein hydrolysis in natural green olives: Importance of the endogenous enzymes. Food Chem. 206, 204-209. https://doi.org/10.1016/j.foodchem.2016.03.061 PMid:27041317

Rigane G, Salem RB, Sayadi S, Bouaziz M. 2011. Phenolic composition, isolation, and structure of a new deoxyloganic acid derivative from Dhokar and Gemri-Dhokar olive cultivars. J. Food Sci. 76, C965-C973. https://doi.org/10.1111/j.1750-3841.2011.02290.x PMid:21806611

Romero C, Ruiz-Méndez MV, Brenes M. 2016. Bioactive Compounds in Virgin Olive Oil of the PDO Montoro-Adamuz, J. Am. Oil Chem. Soc. 93, 665-672. https://doi.org/10.1007/s11746-016-2803-z

Romero-Segura C, Sanz C, Perez AG. 2009. Purification and characterization of an olive fruit β-glucosidase involved in the biosynthesis of virgin olive oil phenolics. J. Agric. Food Chem. 57, 7983-7988. https://doi.org/10.1021/jf901293c PMid:19689134

Romero-Segura C, García-Rodríguez R, Sánchez-Ortiz A, Sanz C, Pérez AG. 2012. The role of olive β-glucosidase in shaping the phenolic profile of virgin olive oil. Food Res. Internat. 45, 191-196. https://doi.org/10.1016/j.foodres.2011.10.024

Sousa A, Malheiro R, Casal S, Bento A, Pereira JA. 2015. Optimal harvesting period for cvs. Madural and Verdeal Transmontana, based on antioxidant potential and phenolic composition of olives. LWT-Food Sci. Technol. 62, 1120-1126. https://doi.org/10.1016/j.lwt.2015.01.046

Susamci E, Ozturk Gungor F, Irmak S, Ataol Olmez H, Tusu G. 2016. A study on the nutritional value of Hurma olives (Erkence cv.) that lose the bitterness on the tree. J. Agric. Sci. 22(4), 471-479. https://doi.org/10.1501/Tarimbil_0000001405

Susamci E, Romero C, Tuncay O, Brenes M. 2017. An explanation for the natural de-bittering of Hurma olives during ripening on the tree. Grasas Aceites 68, 1-8. https://doi.org/10.3989/gya.1161162

Talhaoui N, Gómez-Caravaca AM, León L, De La Rosa R, Fernández-Gutiérrez A, Segura-Carretero A. 2015. Pattern of variation of fruit traits and phenol content in olive fruits from six different cultivars. J. Agric. Food Chem. 63, 10466-10476. https://doi.org/10.1021/acs.jafc.5b04315 PMid:26509962

Tombesi A, Boco M, Pilli M. 1998. Fruit microclimate: effect of light on growth and on oil synthesis. Riv. Frutticolt. Ortofloricolt.60(7/8), 63-67.

Uylaser V. 2015. Changes in phenolic compounds during ripening in Gemlik variety olive fruits obtained from different locations. CyTA J. Food 13, 167-173. https://doi.org/10.1080/19476337.2014.931331

Velázquez-Palmero D, Romero-Segura C, García-Rodríguez R, Hernández ML, Vaistij FE, Graham IA, Pérez AG, Martínez-Rivas JM. 2017. An oleuropein β-glucosidase from olive fruit is involved in determining the phenolic composition of virgin olive oil, Front. Plant Sci. 8, 1-12. https://doi.org/10.3389/fpls.2017.01902 PMid:29163620 PMCid:PMC5682033