Extracción ecológica de compuestos fenólicos de orujo de aceituna: optimización de las condiciones de autohidrólisis y evaluación de aplicaciones potenciales
DOI:
https://doi.org/10.3989/gya.0213251.2292Palabras clave:
5-hidroximetilfurfural, Antibiopelícula, Antioxidantes, Autohidrólisis, Hidroxitirosol, Orujo de olivaResumen
Este estudio evaluó el uso de la autohidrólisis como método ecológico para la extracción de biofenoles con actividad antioxidante y antibiofilm del orujo de aceituna (OP) uruguayo, considerando la formación de compuestos de degradación como el 5-hidroximetilfurfural (HMF). Se empleó la metodología de superficie de respuesta para evaluar el efecto de la temperatura, el tiempo de extracción y la relación líquido-sólido sobre el rendimiento de extracción (EY), el contenido fenólico total (TPC), la actividad antioxidante (ensayos FRAP y DPPH), el contenido de hidroxitirosol (HTY) y el contenido de HMF. Se determinaron las condiciones óptimas de extracción (168 °C, 24 min y una relación líquido-sólido de 20 mL/g) para maximizar el EY (25,65 g/100 g), el TPC (28,60 mgGAE/g), la actividad antioxidante (FRAP: 328,50 μmolFSE/g; DPPH: 122,33 μmolTRE/g) y el contenido de HTY (2,79 mg/g), a la vez que se minimizaba el contenido de HMF (0,92 mg/g). En estas condiciones, el extracto obtenido mostró una mayor protección para el aceite de girasol que los antioxidantes sintéticos estudiados (BHA y BHT) y demostró su capacidad para inhibir la formación de biopelículas de Candida albicans y Staphylococcus aureus.
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Datos de los fondos
Agencia Nacional de Investigación e Innovación
Números de la subvención POS_NAC_2018_1_151628
Comisión Sectorial de Investigación Científica
Números de la subvención 2018/85
