Green extraction of phenolic compounds from olive pomace: optimization of autohydrolysis conditions and evaluation of potential applications

L. Cabrera; L. Xavier; I.  Vieitez; S.  Raffaelli; S.  Alborés; B.  Zecchi

doi:10.3989/gya.0213251.2292

Authors

L. Cabrera Instituto de Ingeniería Química. Facultad de Ingeniería. Universidad de la República https://orcid.org/0000-0002-8786-4455
L. Xavier Instituto de Ingeniería Química. Facultad de Ingeniería. Universidad de la República. https://orcid.org/0000-0002-9136-6598
I. Vieitez Área Tecnología de Alimentos. Departamento de Ciencia y Tecnología de Alimentos. Facultad de Química. Universidad de la República https://orcid.org/0000-0003-0320-2970
S. Raffaelli Área de Microbiología. Departamento de Biociencias. Facultad de Química. Universidad de la República https://orcid.org/0000-0002-7194-0855
S. Alborés Área de Microbiología. Departamento de Biociencias. Facultad de Química. Universidad de la República https://orcid.org/0000-0003-3200-3057
B. Zecchi Instituto de Ingeniería Química. Facultad de Ingeniería. Universidad de la República https://orcid.org/0000-0002-6985-3330

DOI:

https://doi.org/10.3989/gya.0213251.2292

Keywords:

5-hydroxymethylfurfural, Antibiofilm, Antioxidants, Autohydrolysis, Hydroxytyrosol, Olive pomace

Abstract

This study evaluated the use of autohydrolysis as a green method to extract biophenols with antioxidant and antibiofilm activities from Uruguayan olive pomace (OP), considering the formation of degradation compounds such as 5-hydroxymethylfurfural (HMF). Response surface methodology was used to evaluate the effect of temperature, extraction time and liquid-solid ratio on extraction yield (EY), total phenolic content (TPC), antioxidant activity (FRAP and DPPH assays), hydroxytyrosol (HTY) content and HMF content. The optimal extraction conditions (168 °C, 24 min, and liquid-solid ratio of 20 mL/g) were determined to maximize EY (25.65 g/100 g), TPC (28.60 mgGAE/g), antioxidant activity (FRAP: 328.50 μmolFSE/g; DPPH: 122.33 μmolTRE/g) and HTY content (2.79 mg/g) while minimizing HMF content (0.92 mg/g). Under these conditions, the obtained extract showed greater protection for sunflower oil than the synthetic antioxidants tested (BHA and BHT) and demonstrated the ability to inhibit the biofilm formation of Candida albicans and Staphylococcus aureus.

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