Comparative chemical composition and antioxidant capacity of essential oil and oleoresin from Eucalyptus globulus Labill. leaves
DOI:
https://doi.org/10.3989/gya.0321251.2302Keywords:
Antioxidant capacity, eucalyptus, hydrodistillation, supercritical CO2, terpenesAbstract
The Eucalyptus globulus is a tree which is cultivated in Peru for its wood, while its leaves are often discarded. This study compared the extraction yield, chemical composition, and antioxidant capacity of essential oil and oleoresin obtained from the leaves. The essential oil was extracted by hydrodistillation (1.62 % yield), and oleoresins were obtained using supercritical CO₂, with and without ethanol as a co-solvent (yield up to 8.96 %). GC-MS analysis identified 1,8-cineole as the major compound in the essential oil and in the oleoresin extracted with supercritical CO₂+ethanol. The essential oil showed a higher monoterpene content (80.33 %), while the oleoresins were richer in sesquiterpenes (up to 63.68 %). The total phenolic content was significantly higher in the oleoresin (5.05-11.30 mg GAE/g) than in the essential oil (0.25 mg GAE/g). Antioxidant capacity was also significantly higher in the oleoresins. These findings highlight the potential of E. globulus oleoresin as a natural antioxidant for food and pharmaceutical applications.
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Abbas A, Anwar F, Alqahtani SM, Ahmad N, Al-Mijalli SH, Shahid M, Iqbal M. 2022. Hydro-distilled and supercritical fluid extraction of Eucalyptus camaldulensis essential oil: Characterization of bioactives along with antioxidant, antimicrobial and antibiofilm activities. Dose-Response 20 (3). https://doi.org/10.1177/15593258221125477 PMid:36106059 PMCid:PMC9465602
Almas I, Innocent E, Machumi F, Kisinza W. 2021. Chemical composition of essential oils from Eucalyptus globulus and Eucalyptus maculata grown in Tanzania. Sci. Afr. 12, e00758. https://doi.org/10.1016/j.sciaf.2021.e00758
Ayed A, Caputo L, De Feo V, Elshafie HS, Fratianni F, Nazzaro F, Hamrouni L, Amri I, Mabrouk Y, Camele I, Polito F. 2024. Antimicrobial, anti-enzymatic and antioxidant activities of essential oils from some Tunisian Eucalyptus species. Heliyon 10, e34518. https://doi.org/10.1016/j.heliyon.2024.e34518 PMid:39113961 PMCid:PMC11303996
Barriga-Sánchez M, Castro-Rumiche CF, Sanchez-Gonzales G, Rosales-Hartshorn MU. 2021. Functional and chemical qualities of Vitis labrusca grape seed oil extracted by supercritical CO2. Rev. Colomb. Quim. 50, 3-9. https://doi.org/10.15446/rev.colomb.quim.v50n3.95469
Barriga-Sánchez M, Varas Condori MA, Churata Huanca AC, Aranda Pariasca DM. 2022. Supercritical Fluid Extraction of Lipids and Astaxanthin Optimization from Munida (Pleuroncodes monodon) and its Characterization. J. Aquat. Food Prod. Technol. 31, 615-624. https://doi.org/10.1080/10498850.2022.2082903
Bendaoud H, Bouajila J, Rhouma A, Savagnac A, Romdhane M. 2009. GC/MS analysis and antimicrobial and antioxidant activities of essential oil of Eucalyptus radiata. J. Sci. Food Agric. 89, 1292-1297. https://doi.org/10.1002/jsfa.3585
Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal. Biochem. 239, 70-76. https://doi.org/10.1006/abio.1996.0292 PMid:8660627
Chahomchuen T, Insuan O, Insuan W. 2020. Chemical profile of leaf essential oils from four Eucalyptus species from Thailand and their biological activities. Microchem J. 158, 105248. https://doi.org/10.1016/j.microc.2020.105248
Čmiková N, Galovičová L, Schwarzová M, Vukic MD, Vukovic NL, Kowalczewski PL, Bakay L, Kluz MI, Puchalski C, Kačániová M. 2023. Chemical composition and biological activities of Eucalyptus globulus essential oil. Plants 12, 1076. https://doi.org/10.3390/plants12051076 PMid:36903935 PMCid:PMC10004840
Elaissi A, Moumni S, Roeleveld K, Larbi Khouja M. 2020. Chemical characterization of five Tunisian Eucaliptus essential oils species. Chem. Biodiversity 17, e1900378. https://doi.org/10.1002/cbdv.201900378 PMid:31845504
Harkat-Madouri L, Asma B, Madani K, Bey-Ould Si Said Z, Rigou P, Grenier D, Allalou H, Remini H, Adjaoud A, Boulekbache-Makhlouf L. (2015). Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Ind. Crops Prod. 78, 148-153. https://doi.org/10.1016/j.indcrop.2015.10.015
Herzi N, Bouajila J, Camy S, Cazaux S, Romdhane M, Condoret JS. 2013. Comparison between supercritical CO2 extraction and hydrodistillation for two species of Eucalyptus: Yield, chemical composition, and antioxidant activity. J. Food Sci. 78, C667-C672. https://doi.org/10.1111/1750-3841.12113 PMid:23551200
Hoch CC, Petry J, Griesbaum L, Weiser T, Werner K, Ploch M, Verschoor A, Multhoff G, Dezfouli AB, Wollenberg B. 2023. 1,8-cineole (eucalyptol): A versatile phytochemical with therapeutic applications across multiple diseases. Biomed. Pharmacother. 167, 115467. https://doi.org/10.1016/j.biopha.2023.115467 PMid:37696087
Immaroh NZ, Kuliahsari DE, Nugraheni SD. 2021. Review: Eucalyptus globulus essential oil extraction method. IOP Conf. Ser.: Earth Environ. Sci. 733, 012103. https://doi.org/10.1088/1755-1315/733/1/012103
Jerbi A, Derbali A, Elfeki A, Kammoun M. 2017. Essential oil composition and biological activities of Eucalyptus globulus leaves extracts from Tunisia. J. Essent. Oil-Bear. Plants 20, 438-448. https://doi.org/10.1080/0972060X.2017.1304832
Khaiper M, Poonia PK, Dhanda SK, Beniwal R, Verma P, Nasir M. 2025. Seasonal variation in chemical composition and bioactivity of Eucalyptus tereticornis leaf essential oil. Biochem. Syst. Ecol. 121, 104988. https://doi.org/10.1016/j.bse.2025.104988
Khaiper M, Poonia PK, Redhu I, Verma P, Sheokand RN, Nasir M, Tiwari A, Kumar V. 2024. Chemical composition, antifungal and antioxidant properties of seasonal variation in Eucalyptus tereticornis leaves of essential oil. Ind. Crop. Prod. 222, 119669. https://doi.org/10.1016/j.indcrop.2024.119669
Koursaoui L, Badr S, Ghanmi M, Jaouadi I, Chibani A, Chahboun N, Aouane EM, Chaouch A, Zarrouk A. 2023. Phytochemical analysis, antioxidant and antimicrobial activity of three Eucalyptus species essential oils from the Moroccan Maâmora Forest: Eucalyptus cladocalyx F.Muell, Eucalyptus grandis W.Hill ex Maiden and Eucalyptus botryoides Sm. Chem. Data Collect. 48, 101101. https://doi.org/10.1016/j.cdc.2023.101101
Laura-Ticona J, Chambi-Rodriguez AD, Coaquira-Quispe JJ. 2024. Efecto antimicrobiano in vitro de aceite esencial de eucalipto (Eucalyptus globulus labill) y muña (Minthostachys mollis). Rev. investig. Altoandin. 26, 36-45. https://doi.org/10.18271/ria.2024.586
Liu S, Zhao C, Cao Y, Li Y, Zhang Z, Nie D, Tang W, Li Y. 2023. Comparison of chemical compositions and antioxidant activity of essential oils from Litsea cubeba, cinnamon, anise, and eucalyptus. Molecules, 28, 5051. https://doi.org/10.3390/molecules28135051 PMid:37446712 PMCid:PMC10343171
Luís Â, Duarte A, Gominho J, Domingues F, Duarte AP. 2016. Chemical composition, antioxidant, antibacterial and anti-quorum sensing activities of Eucalyptus globulus and Eucalyptus radiata essential oils. Ind. Crops Prod. 79, 274-282. https://doi.org/10.1016/j.indcrop.2015.10.055
Mann TS, Babu GDK, Guleria S, Singh B. 2011. Comparison of Eucalyptus cinerea essential oils produced by hydrodistillation and supercritical carbon dioxide extraction. Nat. Prod. Commun. 6, 107-110. https://doi.org/10.1177/1934578X1100600125
Nadeem R, Mumtaz S, Hanif MA, Shahzadi I, Jilani MI. 2016. BIOCHEMICAL CHARACTERISATION OF OLEORESIN OF Eucalyptus citriodora. Oxid. Commun. 39, 3065-3078.
Polito F, Fratianni F, Nazzaro F, Amri I, Kouki H, Khammassi M, Hamrouni L, Malaspina P, Cornara L, Khedhri S, Romano B, Maresca DC, Ianaro A, Ercolano G, De Feo V. 2023. Essential oil composition, antioxidant activity and leaf micromorphology of five Tunisian Eucalyptus species. Antioxidants, 12, 867. https://doi.org/10.3390/antiox12040867 PMid:37107241 PMCid:PMC10135225
Prior RL, Wu X, Schaich K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agric. Food Chem. 53, 4290-4302. https://doi.org/10.1021/jf0502698 PMid:15884874
Procopio FR, Ferraz MC, Paulino BN, do Amaral Sobral PJ, Hubinger MD. 2022. Spice oleoresins as valueadded ingredient for food industry: Recent advances and perspectives. Trends Food Sci. Technol. 122, 123-139. https://doi.org/10.1016/j.tifs.2022.02.010
Rodrigues VH, de Melo MMMR, Tenberg V, Carreira R, Portugal I, Silva CM. 2021. Similarity analysis of essential oils and oleoresins of Eucalyptus globulus leaves produced by distinct methods, solvents and operating conditions. Ind. Crops Prod. 164, 113339. https://doi.org/10.1016/j.indcrop.2021.113339
Rodrigues VH, de Melo MMR, Portugal I, Silva CM. 2018. Extraction of Eucalyptus leaves using solvents of distinct polarity. Cluster analysis and extracts characterization. J. Supercrit. Fluids 135, 263-274. https://doi.org/10.1016/j.supflu.2018.01.010
Sethunga M, Ranaweera K, Gunathilake K, Munaweera I. 2022. Recent advances in the extraction methods of essential oils and oleoresins from plant materials and its potential applications: A comprehensive review. J Food Bioprocess Eng. 5, 151-167.
Shahidi F, Hossain A. 2018. Bioactives in spices, and spice oleoresins: Phytochemicals and their beneficial effects in food preservation and health promotion. Journal of Food Bioactives, 3, 8-75. https://doi.org/10.31665/JFB.2018.3149
Surbhi, Kumar A, Singh S, Kumari P, Rasane P. 2023. Eucalyptus: phytochemical composition, extraction methods and food and medicinal applications. Adv. Tradit. Med. 3, 369-380. https://doi.org/10.1007/s13596-021-00582-7
Usman LA, Oguntoye OS, Ismaeel RO. 2020. Effect of seasonal variation on chemical composition, antidiabetic and antioxidant potentials of leaf essential oil of Eucalyptus globulus L. J. Essent. Oil-Bear. Plants 23, 1314-1323. https://doi.org/10.1080/0972060X.2020.1862710
Yáñez Rueda X, Cuadro Mogollón OF. 2012. Composición química y actividad antibacteriana del aceite esencial de las especies Eucalyptus globulus y E. camaldulensis de tres zonas de Pamplona (Colombia). Rev. Fac. Cienc. Bás., 10, 52-61.
Yousefi M, Rahimi-Nasrabadi M, Pourmortazavi SM, Wysokowski M, Jesionowski T, Ehrlich H, Mirsadeghi S. 2019. Supercritical fluid extraction of essential oils. TrAC, Trends Anal. Chem., 118, 182-193. https://doi.org/10.1016/j.trac.2019.05.038
Zhao S, Zhang D. 2014. Supercritical CO2 extraction of Eucalyptus leaves oil and comparison with Soxhlet extraction and hydro-distillation methods. Sep. Purif. Technol. 133, 443-451. https://doi.org/10.1016/j.seppur.2014.07.018
Zhou L, Li J, Kong Q, Luo S, Wang J, Feng S, Yuan M, Chen T, Yuan S, Ding C. 2021. Chemical composition, antioxidant, antimicrobial, and phytotoxic potential of Eucalyptus grandis × E. urophylla leaves essential oils. Molecules, 26, 1450. https://doi.org/10.3390/molecules26051450 PMid:33800071 PMCid:PMC7962113
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