Physicochemical and spectroscopic characterization, and evaluation of the antioxidant and anti-inflammatory potential of oils extracted from Thunnus thynnus and Sardina pilchardus coproducts

H.  Belkhodja; A.  Remil; F.Z. Labbaci; F.  Belkhadem; D.  Bouhadi; Z. Benattouche

doi:10.3989/gya.0540251.2325

Authors

H. Belkhodja Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli https://orcid.org/0000-0002-1937-2897
A. Remil Laboratory of Organic, Macromolecular chemistry and Materials, University of Mustapha Stambouli https://orcid.org/0009-0006-1694-8711
F.Z. Labbaci Laboratory of physical chemistry of macromolecules and biological interface, University of Mustapha Stambouli https://orcid.org/0000-0002-7362-1022
F. Belkhadem Laboratory of Bioactive Organic Synthesis, Department of Industrial Organic Chemistry, University of Science and Technology of Oran https://orcid.org/0000-0002-7113-7798
D. Bouhadi Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli https://orcid.org/0000-0002-1145-8186
Z. Benattouche Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli https://orcid.org/0000-0002-1937-1894

DOI:

https://doi.org/10.3989/gya.0540251.2325

Keywords:

Coproducts, FTIR-ATR, Oil, Sardina pilchardus, Thunnus thynnus

Abstract

This work aims to valorize the coproducts of two pelagic species, bluefin tuna (Thunnus thynnus) and sardines (Sardina pilchardus), by the extraction and characterization of the oil coproducts through physicochemical and FTIR-ATR analysis and the in vitro evaluation of their antioxidant and anti-inflammatory potential. Determining the physicochemical parameters revealed that both oils tested are of good quality. The UV-visible absorption spectra presented a strong absorbance band at 310 nm. Therefore, oil from S. pilchardus and T. thynnus may predominate ω-3 and 9 in its spectrum. The FTIR-ATR spectrum showed bands and peaks corresponding to functional groups of the substances tested. Evaluation of the antioxidant and anti-inflammatory potential showed that the oil from S. pilchardus coproducts showed the highest reduction percentage and even the highest anti-inflammatory potential compared to T. thynnus. Coproduct oil offers significant therapeutic value as a substitute substance or therapeutic support for reducing oxidative stress and inflammation.

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References

Azeem AK, Dilip C, Prasanth SS, Junise VHS, Sajeev K, Naseera C. 2010. Anti-inflammatory activity of the glandular extracts of Thunnus alalunga. Asian Pac. J. Trop. Med. 3 (10), 794-796. https://doi.org/10.1016/S1995-7645(10)60190-3

Balasa AF, Chircov C, Grumezescu AM. 2020. Marine biocompounds for neuroprotection-A review. Mar. Drugs 18, 290. https://doi.org/10.3390/md18060290 PMid:32486409 PMCid:PMC7344849

Balvers MGJ, Verhoeckx KCM, Plastina P, Wortelboer HM, Meijerink J, Witkamp RF. 2010. Docosahexaenoic acid and eicosapentaenoic acid are converted by 3T3-L1 adipocytes to N-acyl ethanolamines with antiinflammatory properties. Biochim. Biophys. Acta 1801, 1107-1114. https://doi.org/10.1016/j.bbalip.2010.06.006 PMid:20601112

Borges C, Ribeiro A, Carvalho AC, Sousa F, Oliveira I, Batista I, Bandarra NM, Gomes AC, Cavaco-Paulo A. 2014. Study of sardine oil antioxidant properties for the development of topical therapeutic formulations. Planta Med. 80 (S01), P2O67. https://doi.org/10.1055/s-0034-1395057

Cardeira M, Bernardo A, Leonardo IC, Gaspar FB, Marques M, Melgosa R, Paiva A, Simões P, Fernández N, Serra AT. 2022. Cosmeceutical potential of extracts derived from fishery industry residues: Sardine wastes and codfish frames. Antioxidants 11 (10), 1925. https://doi.org/10.3390/antiox11101925 PMid:36290648 PMCid:PMC9598070

Chevrier G, Mitchell PL, Rioux LE, Hasan F, Jin T, Roblet CR, Marette A. 2015. Low-molecular-weight peptides from salmon protein prevent obesity-linked glucose intolerance, inflammation, and dyslipidemia in LDLR−/−/ApoB100/100 mice. J. Nutr. 145 (7), 1415-1422. https://doi.org/10.3945/jn.114.208215 PMid:25995281

Coppola D, Lauritano C, Palma Esposito F, Riccio G, Rizzo C, De Pascale D. 2021. Fish waste: from problem to valuable resource. Mar. Drugs 19 (2), 116. https://doi.org/10.3390/md19020116 PMid:33669858 PMCid:PMC7923225

Dórame-Miranda RF, Gámez-Meza N, Ovando-Martínez M. 2021. Encapsulation of sardine oil by electrospraying with gliadins and pecan nutshell extracts for its stabilization. Food Bioprocess Technol. 14, 457-470. https://doi.org/10.1007/s11947-020-02567-x

El-Shafei R, Hegazy H, Acharya B. 2021. A review of antiviral and antioxidant activity of bioactive metabolites of macroalgae within an optimized extraction method. Energies 14, 3092. https://doi.org/10.3390/en14113092

Hu S, Yuan J, Gao J, Wu Y, Meng X, Tong P, Chen H. 2020. Antioxidant and anti-inflammatory potential of peptides derived from in vitro gastrointestinal digestion of germinated and heat-treated foxtail millet (Setaria italica) proteins. J. Agric. Food Chem. 68, 9415-9426. https://doi.org/10.1021/acs.jafc.0c03732 PMid:32786864

Jesionowska M, Ovadia J, Hockemeyer K, Clews AC, Xu Y. 2023. EPA and DHA in microalgae: health benefits, biosynthesis, and metabolic engineering advances. J. Am. Oil Chem. Soc. 100 (11), 831-842. https://doi.org/10.1002/aocs.12718

Khalihena Groune AL, ElHamidi A, Halim M. 2024. Comparative investigation of fatty acids in the Mauritania sardinella and Moroccan sardine. Curr. Nutr. Food Sci. 20 (1), 85-91. https://doi.org/10.2174/1573401319666221114101642

Lechuga I, Michaelian K. 2023. Fatty acid vesicles as hard UV-C shields for early life. Foundations 3 (1), 99-114. https://doi.org/10.3390/foundations3010010

Maffei S, De Felice C, Cannarile P, Leoncini S, Signorini C, Pecorelli A, Montomoli B, Lunghetti S, Ciccoli L, Durand T. 2014. Effects of ω-3 PUFAs supplementation on myocardial function and oxidative stress markers in typical Rett syndrome. Mediators Inflamm. 2014, 1-8. https://doi.org/10.1155/2014/983178 PMid:24526821 PMCid:PMC3913460

Melgosa R, Trigueros E, Sanz MT, Cardeira M, Rodrigues L, Fernández N, Matias AA, Bronze MR, Marques M, Paiva A. 2020. Supercritical CO₂ and subcritical water technologies for the production of bioactive extracts from sardine (Sardina pilchardus) waste. J. Supercrit. Fluids 164, 104943. https://doi.org/10.1016/j.supflu.2020.104943

Messina CM, Arena R, Manuguerra S, La Barbera L, Curcuraci E, Renda G, Santulli A. 2022. Valorization of side stream products from sea cage fattened bluefin tuna (Thunnus thynnus): Production and in vitro bioactivity evaluation of enriched ω-3 polyunsaturated fatty acids. Mar. Drugs 20, 309. https://doi.org/10.3390/md20050309 PMid:35621959 PMCid:PMC9147267

Mutalipassi M, Esposito R, Ruocco N, Viel T, Costantini M, Zupo V. 2021. Bioactive compounds of nutraceutical value from fishery and aquaculture discards. Foods 10, 1495. https://doi.org/10.3390/foods10071495 PMid:34203174 PMCid:PMC8303620

Nascimento VLV, Bermudez VMS, Oliveira ALL, Kleinberg MN, Ribeiro RTM, Abreu RFA, Carioca JOB. 2015. Characterization of a hydrolyzed oil obtained from fish waste for nutraceutical application. Food Sci. Technol. 35 (2), 321-325. https://doi.org/10.1590/1678-457x.6583

Oliveira CM, dos Santos JG, Costa Sousa RG. 2020. UV spectrophotometry applied to the quantification of omega-3, -6 and -9 in fresh tissues of wild and farmed tambaqui. Int. J. Innov. Educ. Res. 8 (12), 183-195. https://doi.org/10.31686/ijier.vol8.iss12.2831

Pravinkumar M, Eugien LX, Viswanathan C, Raff SM. 2015. Extraction of fish body oil from Sardinella longiceps by employing direct steaming method and its quantitative and qualitative assessment. J. Coast. Life Med. 3 (12), 962-966. https://doi.org/10.12980/jclm.3.201514j71

Pudtikajorn K, Benjakul S. 2020. Simple wet rendering method for extraction of prime quality oil from skipjack tuna eyeballs. Eur. J. Lipid Sci. Technol. 122 (8), 1-29. https://doi.org/10.1002/ejlt.202000077

Purnamayati L, Dewi EN. 2019. Physical blending characteristic of fish oil and sesame oil. IOP Conf. Ser. Earth Environ. Sci. 246, 012054. https://doi.org/10.1088/1755-1315/246/1/012054

Rubio-Rodríguez N, Beltrán S, Jaime I, Diego SM, Sanz MT, Rovira CJ. 2010. Production of omega-3 polyunsaturated fatty acid concentrates: A review. Innov. Food Sci. Emerg. Technol. 11 (1), 1-12. https://doi.org/10.1016/j.ifset.2009.10.006

Rudkowska I, Marcotte B, Pilon G, Lavigne C, Marette A, Vohl MC. 2010. Fish nutrients decrease expression levels of tumor necrosis factor-alpha in cultured human macrophages. Physiol. Genomics 40 (3), 189-194. https://doi.org/10.1152/physiolgenomics.00120.2009 PMid:19952281

Saito S, Numadate N, Teraoka H, Enami S, Kobayashi H, Hama T. 2023. Impurity contribution to ultraviolet absorption of saturated fatty acids. Sci. Adv. 9, 1-7. https://doi.org/10.1126/sciadv.adj6438 PMid:37729407 PMCid:PMC10511181

Strobel SA, Hudnall K, Arbaugh B, Cunniffe JC, Scher HB, Jeoh T. 2020. Stability of fish oil in calcium alginate microcapsules cross-linked by in situ internal gelation during spray drying. Food Bioprocess Technol. 13 (2), 275-287. https://doi.org/10.1007/s11947-019-02391-y

Suseno HS. 2015. Proximate, fatty acid, and mineral composition of tuna (Thunnus sp.) by-product from West Sumatra Province, Indonesia. Pak. J. Nutr. 14 (1), 62-66. https://doi.org/10.3923/pjn.2015.62.66

Trilaksani W, Sinulingga F, Setyaningsih I, Indra Aja Nasution M. 2023. Evaluation of fatty acid quality and antioxidant activity of supplement tablets from tuna eyes oil and Spirulina platensis. Int. J. Chem. Biochem. Sci. 24 (5), 632-638.

Wei L, Li Q, Tan W, Dong F, Luan F, Guo Z. 2017. Synthesis, characterization, and the antioxidant activity of double quaternized chitosan derivatives. Molecules 22, 501. https://doi.org/10.3390/molecules22030501 PMid:28327537 PMCid:PMC6155333

Yates CM, Calder PC, Rainger GE. 2014. Pharmacology and therapeutics of omega-3 polyunsaturated fatty acids in chronic inflammatory disease. Pharmacol. Ther. 141, 272-282. https://doi.org/10.1016/j.pharmthera.2013.10.010 PMid:24201219