Enzymatic aqueous technology for simultaneous coconut protein and oil extraction
DOI:
https://doi.org/10.3989/gya.2003.v54.i1.281Keywords:
Aqueous extraction, Coconut oil, Coconut protein, EnzymeAbstract
In this study, the following commercial enzymes were evaluated in the enzymatic extraction of oil and protein from coconut: Celluclast, Termamyl, Viscozyme, Neutrase y Protease. Preliminary experiments were carried out for selection of enzymes, enzyme concentration and incubation time. In order to verify the effects of pH and water/substrate ratio, a response surface methodology was applied based in a 32 full factorial experimental design. The pH is the most meaningful parameter on oil and protein extraction yields, with a significance level higher than 90%. The maximal extraction yields of oil and protein emulsion (83%) was reached using Viscozyme L and subsequently Neutrase 1.5 MG at concentrations of 0.6% (w/w) and 0.3% (w/w), respectively, total incubation time of 60 minutes, substrate/water ratio 1:6 and pH around 7 (no adjustment).
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Published
2003-03-30
How to Cite
1.
Sant’Anna BP, Freitas SP, Coelho MAZ. Enzymatic aqueous technology for simultaneous coconut protein and oil extraction. grasasaceites [Internet]. 2003Mar.30 [cited 2023Nov.28];54(1):77-80. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/281
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