Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1


  • Hugo S. Garcia Department of Chemical and Biological Engineering, University of Wisconsin-Madison. Madison
  • In-Hwan Kim Department of Food and Nutrition, College of Health Sciences, Korea University, Seoul
  • Arnoldo López-Hernández Department of Chemical and Biological Engineering, University of Wisconsin-Madison. Madison
  • Charles G. Hill, Jr Department of Chemical and Biological Engineering, University of Wisconsin-Madison. Madison




Fish oil - Interesterification, Omega-3, Phospholipids


A commercial phospholipase A1 (Lecitase® Ultra) was immobilized by physical adsorption on Duolite® and then used to mediate the incorporation of omega-3 fatty acids into lecithin. Adsorption isotherms showed that 12 h of contact were sufficient to deposit most of the enzyme onto the carrier. A pH of 7 and 50°C were the best conditions for adsorption. Reaction mixtures consisting of lecithin and a saponified fish oil concentrate (78.4 mol % EPA+DPA+DHA) were prepared at molar ratios ranging from 1:2 to 1:10. Typically 2 g of total substrates and 200 mg of enzyme preparation were employed in batch reactor trials. The fastest reaction rates were observed when a substrate mole ratio of 1:8 (lecithin:total fatty acids) was employed. Use of the enzyme preparation dried at pH 8 and reaction temperatures of 50 and 60°C produced the greatest extent of incorporation of the indicated n-3 fatty acids into the phospholipid after 24h of reaction.


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How to Cite

Garcia HS, Kim I-H, López-Hernández A, Hill, Jr CG. Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1. grasasaceites [Internet]. 2008Dec.30 [cited 2023Feb.7];59(4):368-74. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/531




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