Effects of accelerated aging upon the lipid composition of seeds from two soft wheat varieties from Morocco
Keywords:Accelerated aging, Deterioration, Fatty acids, Wheat
The lipid composition of the seeds from two soft wheat varieties (Triticum aestivum, cv. Marchouche and Mahdia) were analyzed before and after accelerated aging. Eight days of accelerated aging resulted in a total inhibition of seed germinability as well as a decrease in their total and especially unsaturated fatty acid contents. Oleic and linoleic acid contents decreased particularly in the phosphatidylcholine of the seeds from both varieties. The proportion of polar lipids also decreased after aging as compared to neutral lipids: a 5.8% and 7.2% decrease in polar lipids was e observed in Mahdia and Marchouche cultivars, respectively. In the neutral lipids of the seeds from the Marchouche variety, the percentage of free fatty acids increased whereas the triacylglycerols decreased. After aging, the fatty acid compositions of all lipid classes were modified in the same manner as total fatty acid compositions. Among polar lipids, phospholipid proportions were particularly small, especially the phosphatidylcholine percentages with an 18.1% and 19.1% decrease in Mahdia and Marchouche varieties, respectively. In contrast, MGDG percentages increased, especially in the seeds from the Marchouche variety. A 15.5% increase was noticed when compared with seeds which were not aged. At the same time, the DGDG percentage showed a 16.6% decrease after accelerated aging of the seeds from the Marchouche variety. From these results we concluded that the lipid content decrease observed in seeds after accelerated aging could be linked to a loss in the germination and vigor of wheat seeds.
Bailly C, Benamar A, Corbineau F and Côme D 1996. Changes in malondialdehyde content, superoxide dismutase, catalase and gluthatione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiol Plantarum 97, 104-110. doi:10.1111/j.1399-3054.1996.tb00485.x
Barnes PJ 1983.Wheat germ oil. In Barnes PJ (Ed), Lipids in Cereal Technology. Pp.156-169, Academic Press, New York, USA.
Bligh EG and Dyer WJ. 1959. A rapid method of total lipid extraction and purification, Can. J. of Biochem and Physiol 37, 911-117.
Corbineau F, Gay-Mathiey M, Vinel D and Côme, D 2002. Decrease in sunflower (Helianthus annuus) seed viability caused by high temperature as related to energy metabolism, membrane damage and lipid composition. Physiol. Plantarum 116, 489-496. doi:10.1034/j.1399-3054.2002.1160407.x
Delouche JC and Baskin CC 1973. Accelerated aging techniques for predicting the relative storability of seed lots. Seed. Sci. and Technol. 1, 427-452.
Demandre C, Bahl J, Serghini H, Alpha M J and Mazliak P. 1994 Phosphatidylcholine molecular species formed by lysophosphatidylcholine acyltransferase from soya beam microsomas. Phytochem 35, (5) 1171-1175. doi:10.1016/S0031-9422(00)94816-7
Devaiah SP, Pan X, Hong Y, Roth M, Welti R and Wang X. 2007. Enhancing seeds quality and viability by suppressing phospholipase D in Arabidopsis. Plant J. 50, 950-957. doi:10.1111/j.1365-313X.2007.03103.x PMid:17565616
Falcone DL, Ogas JP and Somerville C.R. 2004. Regulation of membrane fatty acid composition by temperature in mutants of Arabidopsis with alterations in membrane lipid composition. BMC Plant Biol. 4, 17-31. doi:10.1186/1471-2229-4-17 PMid:15377388 PMCid:524174
Francis A and Coolbear P. 1984. Changes in the membrane phospholipid composition of Tomato seeds accompanying loss of germination capacity caused by controlled deterioration. J. of Exp. Bot. 35, 1764- 1770. doi:10.1093/jxb/35.12.1764
Freitas R A, Dias D C F S, Dias LAS and Oliveira MGA. 2006. Alterações fisiológicas e bioquímicas em sementes de algodão submetidas ao envelhecimento artificial. Bioscience J., Uberlândia 22, 67-76.
Gaudillat M 1985. La conductimétrie. In Pradet, A. and Saint-Ges, V. (eds) Les Méthodes d’Evaluations de la Qualité des Semences. Pp. 47- 59, INRA, Bordeaux, France.
Gidrol X, Serghini H, Noubhani A, Mocquot B and Pradet A. 1989. Biochemical changes induced by accelerated aging in sunflower seeds : I. Lipid peroxydation and membrane damage. Physiol Plantarum 76, 598-604. doi:10.1111/j.1399-3054.1989.tb05484.x
Goel A, Goel AK and Sheoran IS 2003. Change in oxidative stress enzymes during artificial aging in cotton (Gossypium hirsutum L.) seeds. J. of Plant Physiol. 160, 1093-1100. doi:10.1078/0176-1617-00881 PMid:14593811
Goesaert H, Brijs K, Veraverbeke WS, Courtin CM, Gebruers K and Delcour JA. 2005. Wheat flour constituents: how they impact bread quality, and how to impact their functionality. Trends in Food Sci. and Technol. 16, 12-30. doi:10.1016/j.tifs.2004.02.011
Halder S and Gupta K. 1980. Effect of storage of sunflower seeds in high and low relative humidity on solute leaking and internal biochemical changes. Seed Sci. and Technol. 8, 317-321.
Harman G E and Mattick LR. 1976. Association of lipid oxidation with seed aging and death. Nature 260, 323- 324. doi:10.1038/260323a0
Harwood JL, Jones A L, Perry HJ, Rutter AJ, Smith KM and Williams M. 1994. Changes in lipid during temperature adaptation. In Cossins, A.R. (Ed) Temperature adaptation of biological membranes. Pp.107-117, Portland Press, London, England.
Kibinza S, Vinel D, Côme D, Bailly C and Corbineau F. 2006. Sunflower seed deterioration as related to moisture content during aging, energy metabolism and active oxygen species scavenging. Physiol. Plantarum 128, 496-506. doi:10.1111/j.1399-3054.2006.00771.x
Konopka I, Rotkiewicz D, Ta>ska M. 2005. Wheat endosperm hardness. Part II. Relationships to content and composition of flour lipids. Eur. Food Res. and Technol. 220, 20-24. doi:10.1007/s00217-004-1038-7
Kumar GNM, Knowles NR. 1993. Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of Potato (Solanum tuberosum) seed-tubers. Plant Physiol. 102. 115-124.
Lehner A, Mamadou N, Poels P, Côme D, Bailly C and Corbineau F. 2007. Changes in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activities in the embryo during aging in wheat grains. J. of Cereal Sci. 47, 555-565. doi:10.1016/j.jcs.2007.06.017
Lepage M. 1967. Identification and composition of turnip root lipids. Lipids 2, 244-250. doi:10.1007/BF02532563 PMid:17805774
Leshem YY. 1987. Membrane phospholipid catabolism and Ca2_ activity in control of senescence. Physiol. Plantarum 69, 551-559. doi:10.1111/j.1399-3054.1987.tb09239.x
Mangold HK. 1961. Thin layer chromatography of lipids. J. Am. Oil Chem. Soc. 38, 708-724. doi:10.1007/BF02633061
Mazliak P. 1983. Plant membrane lipids: changes and alterations during aging and senescence. In Liebermann M. (Ed) Postharvest Physiology and Crop Preservation. Pp. 123-140, Plenum Press, New York, USA.
McDonald MB. 1999. Seed deterioration physiology, repair and assessment. Seed Sci. and Technol. 27, 177-237.
McKersie BD, Hoekstra FA and Krieg LC 1990. Differences in the susceptibility of plant membrane lipids to peroxidation. BBA -Biomembranes 1030, 119-126.
Narayana Murthy UM and Sun WQ. 2000. Protein modification by Amadori and Maillard reactions during seed storage: roles of sugar hydrolysis and lipid peroxidation. J. of Exp. Bot. 51, 1228-1228. doi:10.1093/jexbot/51.348.1221 PMid:10937697
Palt, JP, Levitt J, and Stadelmann EJ. 1977. Freezing injury in onion bulb cells. I. Evaluation of the conductivity method and analysis of ion and sugar efflux from injured cells. Plant Physiol. 60, 393–397. doi:10.1104/pp.60.3.393 PMid:16660100 PMCid:542623
Parrish DJ, Leopold AC 1978. On the mechanism of total lipid extraction and purification. Can J. of Biochem. and Physiol. 37, 911-917.
Pearce RS, Abdel Samad IM. 1980. Change in fatty acid content of polar lipids during aging of seeds of peanut (Arachis hypogea, L.). J. of Exp. Bot. 31, 1283-1290. doi:10.1093/jxb/31.5.1283
Powell AA, Matthews S. 1981. Association of phospholipids changes with early stages of seed aging. Ann. Bot. 47, 709-712.
Ruibal-Mendieta NL, Delacroix DL, Meurens M. 2002. A comparative analysis of bound and total lipid content on spelt and winter wheat wholemeal. J. of Cereal Sci. 35, 337-342. doi:10.1006/jcrs.2001.0434
Somerville C and Browse J. 1991. Plant lipids, metabolism and membranes. Sci. 252, 80-88. doi:10.1126/science.252.5002.80 PMid:17739077
Stewart RRC and Bewley JD. 1980. Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiol. 65, 245-248. doi:10.1104/pp.65.2.245 PMid:16661168 PMCid:440305
Tammela P, Hopia A, Hiltunen R, Vuorela H and Nygren M. 2000. Aging in Pinus sylvestris L. seeds : changes in viability and lipids. Biochem. Soc. Trans. 28, 878- 879. doi:10.1042/BST0280878 PMid:11171242
Zacheo G, Cappello, AR, Perrone LM and Gnoni GV. 1998. Analysis of factors influencing lipid oxidation of almond seeds during accelerated aging. Lebensm. Wiss Technol. 31, 6-9. doi:10.1006/fstl.1997.0289
Zacheo G, Cappello MS, Gallo A, Santino A and Cappello AR. 2000. Changes associated with post-harvest aging in Almond Seeds. Lebensm. Wiss Technol. 33, 415-423. doi:10.1006/fstl.2000.0679
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