Antioxidant activity of wild edible plants in the Black Sea Region of Turkey


  • Tevfik Özen Giresun University, Department of Chemistry, Faculty of Arts and Sciences



Antioxidant activity, Anthocyanins, Black Sea Region, Flavonoids, Total phenolic compounds, Wild edible plants


The antioxidative activity of the 80% ethanol extract obtained from eleven commonly consumed wild edible plants was determined according to the phosphomolybdenum method, reducing power, metal chelating, superoxide anion and free radical scavenging activity and compared to standard compounds such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and trolox. Total phenolic compounds, flavonoids, and anthocyanins in the extracts were also measured. Trachystemon orientalis, Vaccinium mrytillus, Rumex acetosella Polygonum amphibium, Beta vulgaris, and Similax Excelsa had the highest antioxidant capacities. Overall results showed that these plants can serve as good sources of bioactive polyphenols in the human diet and can be regarded as good candidates for nutritional supplement formulations due to their high concentrations of total phenolic compounds, flavonoids and anthocyanins as well as their strong antioxidant activity.


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Aruoma OI. 1998. Free radicals, oxidative stress, and antioxidants in human health and disease. J. Am. Oil Chem. Soc. 75, 199-212. doi:10.1007/s11746-998-0032-9

Benavente-García O, Castillo J, Marin FR, Ortuño A, Del Río JA. 1997. Use and properties of Citrus flavonoids. J. Agric. Food Chem. 45, 4505-4515. doi:10.1021/jf970373s

Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200. doi:10.1038/1811199a0

Branen AL. 1975. Toxicology and biochemistry of butylated hydroxy anisole and butylated hydroxy toluene. J. Am. Oil Chem. Soc. 52, 59-63. doi:10.1007/BF02901825 PMid:805808

Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J. Food Drug Anal. 10, 178-182.

Chun OK, Kim DO, Smith N, Schroeder D, Han JT, Lee CY. 2005. Daily consumption of phenolics and total antioxidant capacity from fruit and vegetables in the American diet. J. Sci. Food Agr. 85, 1715-1724. doi:10.1002/jsfa.2176

Cakir A, Mavi A, Kazaz C, Yildirim A, Kufrevioglu OI. 2006. Antioxidant activities of the extracts and components of Teucrium orientale L. var. orientale. Turk J. Chem. 30, 483–494.

Cos P, Ying LY, Calomme M, Hu JH, Cimanga K, Van Poel B, Pieters L, Vlietinck AJ, Berghe DV. 1998. Structure activity relationships and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers. J. Nat. Prod. 61, 71-76. doi:10.1021/np970237h PMid:9461655

Deshpande SS, Deshpande US, Salunkhe DK. 1996. Nutritional and health aspects of food antioxidants. In D. L. Madhavi, S. S. Deshpande, & D. K. Saunkhe (Eds.), Food antioxidants (pp. 361-469). New York, USA: Marcel Dekker.

Dinis TCP, Madeira VMC, Almeida LM. 1994. Action of phenolic derivatives (acetaminophen, salicylate and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation as peroxyl radical scavenging effects. Chem. Pharm. Bull. 36, 2090-2097.

Dorman HJD, Kosar M, Kahlos K, Holm Y, Hiltunen R. 2003. Antioxidant properties of composition of aqueous extracts from Mentha species, hybrids, vareties, and cultivars. J. Agr. Food Chem. 51, 4563-4569. doi:10.1021/jf034108k PMid:14705878

Duh PD, Tu YY, Yen GC. 1999. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). LWT- Food Sci. Technol. 32, 269-277.

Einbonda LS, Reynertsona KA, Luo XD, Basileb MJ, Kennellya EJ. 2004. Anthocyanin antioxidants from edible fruits. Food Chem. 84, 23-28. doi:10.1016/S0308-8146(03)00162-6

Goa X, Brörk L, Trajkovski V, Uggla M. 2000. Evcaluation of antioxidsant activities of rosehip ethanol extract in different test systems. J. Agr. Food Chem. 80, 2021-2027. doi:10.1002/1097-0010(200011)80:14<2021::AID-JSFA745>3.0.CO;2-2

Güner A, Özhatay N, Ekim T, Baser KHC. 2000. Flora of Turkey and The East Aegean Islands, Vol. 11. Edinburgh, Edinburgh University Press.

Gordon MH. 1990. The mechanism of antioxidant action in vitro. In: B.J.F. Hudson, Editor, Food antioxidants, Elsevier Science, London, pp. 1-18.

Haber F, Weiss J. 1934. The catalytic decomposition of hydrogen peroxide by iron salts. Proceedings of the Royal Society London 147, 332-351. doi:10.1098/rspa.1934.0221

Hall CA, Cuppet SL. 1997. Strctureactivities of natural antioxidants. In: Antioxidant Methodology in vivo and in vitro Concepts. Aruoma 01, Cuppet SL (eds). Champaign, IL, pp. 2-29.

Halliwell B, Aeschbech R, Löliger J, Aruoma OI. 1995. The characterization of antioxidants. Food Chem. Toxicol. 33, 601-617. doi:10.1016/0278-6915(95)00024-V

Halliwell B. Gutteridge JMC. 1999. Free Radicals in Biology and Medicine, 3rd ed. Oxford University Press, Oxford.

Hu FB. 2000. Dietary pattern analysis: a new direction in nutritional epidemiology. Curr. Opin. Lipidol. 13, 3-9. doi:10.1097/00041433-200202000-00002 PMid:11790957

Ito N, Fukushima S, Hasegawa A, Shibata M, Ogiso T. 1983. Carcinogenicity of butylated hydroxy anisole in F344 rats. J. Natl. Cancer I. 70, 343-347.

Kumar, PS, Sucheta S, Deepa, VS, Selvamani P, Latha S. 2008. Antioxidant activity in the some selected Indian medical plants. Afr. J. Biotechnol. 7, 12, 1826-1828.

Kumpulainen JT, Salonen JT. 1999. Natural Antioxidants and Anticarcinogens in Nutrition, Health and Disease. The Royal Society of Chemistry, K pp 178-187.

Lee J, Durst RW, Wrolstad RE. 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. J. AOAC Int. 88, 1269-1278. PMid:16385975

Liu RH. 2003. Health benefits of fruits and vegetables are from additive and synergistic combination of phytochemicals. Am. J. Clin. Nutr. 78, 517S–520S. PMid:12936943

Löliger J. 1991. The use of antioxidants in foods. In Free Radicals and Food Additives; Arouma, O. I., Halliwell, B., Eds.; Taylor and Francis: London, pp 121-150.

Matthaus B. 2002. Antioxidant activity of extracts obtained from residues of different oilseeds. J. Agric. Food Chem. 50, 3444-3452. doi:10.1021/jf011440s

Meir S, Kanner J, Akiri B, Hadas S P. 1995. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agr. Food Chem. 43, 1813-1817. doi:10.1021/jf00055a012

Negi PS, Jayaprakasha GK, Jena BS. 2003. Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem. 80, 393–397. doi:10.1016/S0308-8146(02)00279-0

Ng T B, Liu F, Wang ZT. 2000. Antioxidative activity of natural products from plants. Life Sci. 66, 8, 709-723. doi:10.1016/S0024-3205(99)00642-6

Nishimiki M, Rao NA, Yagi K. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Bioph. Res. Co. 46, 849-853. doi:10.1016/S0006-291X(72)80218-3

Oyaizu M. 1986. Studies on products of browing reaction: Antioxidative activity of product of browing reaction preapared from glucosamine. Jap. J. Nutr. 44, 307-315.

Ozbucak TB, Akçin ÖE, Yalçın S. 2007. Nutrition Contents of the Some Wild Edible Plants in Central Black Sea Region of Turkey. Int. J. Nat. Eng. Sci. 1, 11-13.

Pardini RS. 1995. Toxicity of oxygen from naturally occurring redox-active pro-oxidants. Arch. Insect Biochem. Physiol. 29, 101–118. doi:10.1002/arch.940290203 PMid:7606039

Prieto, P., Pineda, M., Aguilar, M. 1999. Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E, Anal. Biochem. 269, 337-341. doi:10.1006/abio.1999.4019 PMid:10222007

Sekercioglu N, Ozkutlu F, Deveci M, Dede O, Yilmaz N. 2006. Evalution of some wild plants aspect of their nutritional values used as vegetable in the Eastern Black Sea Region of Turkey. Asian J. Plant Sci. 5(2), 185-189. doi:10.3923/ajps.2006.185.189

Sezik E, Yesilada E, Honda G, Takaishi Y, Takeda Y, Tanaka T. 2001. Traditional medicine in Turkey. X. Folk medicine in central Anatolia. J. Ethnopharmacology, 75, 95-115. doi:10.1016/S0378-8741(00)00399-8

Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult. 16, 144-158.

Velioglu YS, Mazza G, Gao L, Oomah B. 1998. Antioxidant activity and total polyphenolics in selected fruits, vegetables, and grain products. J. Agr. Food Chem. 46 (10), 4113–4117. doi:10.1021/jf9801973

Yamaguchi F, Ariga T, Yoshimira Y, Nakazawa H. 2000. Antioxidant and antilycation of carcinol from Garcinia indica fruit rind. J. Agric. Food Chem. 48, 180-185. doi:10.1021/jf990845y PMid:10691613

Winterbourn CC, Kettle AJ. 2003. Radical-radical reactions of superoxide: a potential route to toxicity. Biochem. Biophy. Res. Com. 305, 729–736. doi:10.1016/S0006-291X(03)00810-6




How to Cite

Özen T. Antioxidant activity of wild edible plants in the Black Sea Region of Turkey. grasasaceites [Internet]. 2010Mar.30 [cited 2023Feb.7];61(1):86-94. Available from: