Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases.

Authors

  • Alvaro Villanueva Instituto de la Grasa.
  • Alfonso Clemente Instituto de la Grasa.
  • Juan Bautista Department of Biochemistry, Bromatology and Toxicology. University of Seville
  • Francisco Millán Instituto de la Grasa.

DOI:

https://doi.org/10.3989/gya.1999.v50.i6.697

Keywords:

Alcalase, Flavourzyme, Protein hydrolysate, Protein isolate, Sunflower.

Abstract


A high quality protein isolate has been obtained from defatted sunflower meal by alkaline extraction and isoelectric precipitation. Protein content was increased from 31.2 % in the defatted flour to 97 % in the protein isolate. The percentages of fiber, soluble sugars, polyphenols and residual lipids in the protein isolate were reduced to more than 90 % with respect to the defatted meal. The protein isolate was used as starting material for the generation of an extensive enzymatic protein hydrolysate. The hydrolysis was carried out in a pH stat using sequentially an endo-protease (Alcalase) and an exo-protease (Flavourzyme). The protein hydrolysate, with a degree of hydrolysis of 50.7 %, was white and non bitter.

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Published

1999-12-30

How to Cite

1.
Villanueva A, Clemente A, Bautista J, Millán F. Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases. grasasaceites [Internet]. 1999Dec.30 [cited 2022Jan.19];50(6):472-6. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/697

Issue

Section

Research

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