Obtention of highly purified fractions of eicosapentaenoic acid and docosahexaenoic acid from sardine oil by silver-resin chromatography. A semipreparative procedure.

Authors

  • S. Nieto Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile
  • Ana M. Córdoba Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile
  • J. Sanhueza Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile
  • A. Valenzuela Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile

DOI:

https://doi.org/10.3989/gya.1997.v48.i4.789

Keywords:

Docosahexaenoic acid, Eicosapentaenoic acid, Sardine oil, Semi-preparative procedure, Silver-resin column chromatography.

Abstract


Pure fractions of eicosapentaenoic acid (ERA) and docosahexaenoic acid (DHA) were obtained from silver-resin column chromatography of ERA+DHA concentrates obtained from sardine oil. Two types of silver-impregnated resins were assayed; Amberlite IR-118H and Dowex 50 W-HCR-W2.The Amberlite-silver column allows the separation of almost pure fractions (98.5%) of ERA, being unable to separate DHA from other polyunsaturated fatty acids. The Dowex-silver column does not separate the ERA from the concentrate but allows the isolation of a highly purified fraction of DHA. The chromatographic procedure described may provide pure forms of two important n-3 polyunsaturated fatty acids for nutritional or pharmacological research or as substrates for the obtention of structured lipids.

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Published

1997-08-30

How to Cite

1.
Nieto S, Córdoba AM, Sanhueza J, Valenzuela A. Obtention of highly purified fractions of eicosapentaenoic acid and docosahexaenoic acid from sardine oil by silver-resin chromatography. A semipreparative procedure. grasasaceites [Internet]. 1997Aug.30 [cited 2023Feb.6];48(4):197-9. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/789

Issue

Section

Research

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