Some Biological Compounds, Radical Scavenging Capacities and Antimicrobial Activities in the seeds of Nepeta italica L. and Sideritis montana L. subsp. montana from Turkey
DOI:
https://doi.org/10.3989/gya.033210Keywords:
Antimicrobial Activity, DPPH radical scavenging activity, Fatty acids, Flavonoids, Nepeta italica L., Vitamins, Sideritis montana L. subsp. montana, SterolsAbstract
This study determined some biological compounds (fatty acid compositions, lipid-soluble vitamins, sterols, flavonoids), radical scavenging capacities and antimicrobial activities in the seeds of Nepeta italica L. and Sideritis montana L. subsp. montana. It was found that palmitic acid (C16:0; 8.54±0.13- 3.05±0.04%), oleic acid (C18:1 n9, 22.41±0.8-18.83±0.1%) and α-linolenic acid (C18:3 n3;39.56±0.67-77.04±2.07%) were the dominant fatty acids in both Nepeta italica L. and Sideritis montana L. subsp. montana. It was concluded that both Nepeta italica L. and Sideritis montana L. subsp. montana contained stigmasterol (630.07±1.81µg/g, 80.74±0.71µg/g, respectively) and ergosterol (1.11±0.14µg/g, 161.32±0.63µg/g respectively) as well as beta-sitosterol (2.93±0.03 µg/g). The present findings show that Nepeta italica L. contains morin (37.79±1.09μg/g), catechin (124.39±2.23µg/g), naringin (475.96±3.57µg/g) and Sideritis montana L. subsp. montana contains morin (188.41±2.53µg/g), catechin (64.14±1.86μg/g), naringenin (38.34±1.78μg/g) as major flavonoids. It was also determined that methanol extracts of Nepeta italica L. and Sideritis montana L. subsp. montana were most effective against DPPH radicals. The results of the present study show that the vitamins, flavonoids and fatty acid extracts in the seeds of N. italica L. and S. montana L. subsp. montana prevented the growth of the microorganisms used in the tests at different ratios.
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References
Aboutabl EA, Nassar MI, Elsakhawy FM, Maklad YA, Osman AF, El-Khrisky EAM. 2002. Phytochemical and pharmacological studies on Sideritis taurica Stephen ex Wild. Journal of Ethnopharmacology 82, 177-184. doi:10.1016/S0378-8741(02)00172-1
Ai-li J, Chang-Hai W. 2006. Antioxidant properties of natural components from Salvia plebeia on oxidative stability of ascidian oil. Process Biochemistry 41, 1111-1116. doi:10.1016/j.procbio.2005.12.001
Akpınar N, Akpınar MA, Gorgun S, Dirmenci T, Aktumsek A. 2008. Fatty acid composition of the seeds of five Nepeta species from Turkey. Chemistry of Natural Compounds 44 (1) 90-92. doi:10.1007/s10600-008-0025-9
Azcan N, Ertan A, Demirci B, Bas¸er KHC. 2004. Fatty acid composition of seed oils of twelve Salvia species growing in Turkey. Chemistry of Natural Compounds 40 (3) 218-221. doi:10.1023/B:CONC.0000039127.56323.3e
Barros L, Heleno SA, Carvalho AM, Ferreira ICFR. 2009. Lamiaceae often used in Portuguese folk medicine as a source of powerful antioxidants: Vitamins and phenolics. LWT - Food Science and Technology 1-7.
Basile A, Senatore F, Gargano R, Sorbo S, Del Pezo M, Lavitola A, Ritieni A, Bruno M, Spatuzzi D, Rigano D, Vuotta ML. 2006. Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils. Journal of Ethnopharmacology 107, 240–248. doi:10.1016/j.jep.2006.03.019 PMid:16690235
Bouayed J, Piri K, Rammal H, Dicko A, Desor F, Younos C, Soulimani R. 2007. Comparative evaluation of the antioxidant potential of some Iranian medicinal plants. Food Chemistry 104, 364-368. doi:10.1016/j.foodchem.2006.11.069
Cantino PD. And Sanders, RW. 1986. Subfamilial classification of Labiatae. Syst Bot. 11, 163–185. doi:10.2307/2418955
Christie WW. 1990.Gas Chromatography and lipids. The oily press: Glaskow: UK 573-577.
Collins CH, Lyne PM. 1987. Microbiological Methods Butter Morths & Co (Publishers) Ltd. London, pp. 450.
Davis P.H. 1982. Flora of Turkey and the Aegean Islands. V: 7, Edinburgh Univ. Press., England.
Demo A, Petrakis C, Kefalas P, Boskou D. 1998. Nutrient antioxidants in some herbs and Mediterranean plant leaves. Food Research International 31 (5) 351-354. doi:10.1016/S0963-9969(98)00086-6
Diaz RM, Garcia-Granados A, Moreno E, Parra A, Quevedo-Sarmiento J, Saenz de Buruaga A, Saenz de Buruaga JM. 1988. Studies on the relationship of structure to antimicrobial properties of diterpenoid compound from Sideritis. Planta Medica 54, 301–304. doi:10.1055/s-2006-962439
Erdemoglu N, Turan, NN, Cakıcı I, Bilge Sener B, Aydın A. 2006. Antioxidant Activities of Some Lamiaceae Plant Extracts. Phytother. Res. 20, 9–13. doi:10.1002/ptr.1816 PMid:16397914
Ertan A, Azcan N, Demirci B, Baser KHC. 2001. Fatty acid composition of Sideritis species. Chemistry of Natural Compounds 37 (4) 301-303. doi:10.1023/A:1013775213228
Ezer N, Abbasoglu U. 1996. Antimicrobial activity of essential oils of some Sideritis species growing in Turkey. Fitoterapia 67, 474–475.
Giao MS, Gonzales-Sanjose ML, Rivero-Perez MD, Pereira CI, Pintado ME. and Xavier MF. 2007. Infusions of Portuguese medicinal plants: dependence of final antioxidant capacity and phenol content on extraction features. Journal of the Science of Food and Agriculture 87, 2638-2647. doi:10.1002/jsfa.3023 PMid:20836172
Grzegorczyk I, Matkowski A, Wysokinska H, 2007. Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chemistry 104, 536–54. doi:10.1016/j.foodchem.2006.12.003
Güvenc A, Okada Y, Ku.peli Akkol E, Duman H, Okuyama T, Çalıs I. 2010. Investigations of anti-inflammatory, antinociceptive, antioxidant and aldose reductase inhibitory activities of phenolic compounds from Sideritis brevibracteata. Food Chemsitry 118, 686–692. doi:10.1016/j.foodchem.2009.05.034
Hara A, Radin NS. 1978. Lipid extraction of tissues with a low-toxicity solvent. Anal.Biochem. 90 (1) 420-426. doi:10.1016/0003-2697(78)90046-5
Hernandez-Perez M, Rabanal RM. 2002. Evaluation of the antinflammatory and analgesic activity of Sideritis canariensis var. pannosa in mice. Journal of Ethnopharmacology 81, 43-47. doi:10.1016/S0378-8741(02)00033-8
Hussain J, Ulah F, Hussain H, Hussain S.T. and Shah M.R. 2005. Nepetolide:A new diterpene from Nepeta suavis. Z. Naturforsch. 63 (b) 591-594.
Kiliç T, Dirmenci T, Gören AC. 2007. Fatty acids of seeds of some species of Nepeta L. Natural Product Research 21 (5) 465-468. doi:10.1080/14786410601129473 PMid:17487620
Klimek B. and Modnicki D. 2005. Terpenoids and sterols from Nepeta cataria L. var. citriodora (Lamniaceae). Acta Pol Pharm. 62 (3) 231-235. PMid:16193817
Koleva II, Linssen JPH, van Beek TA, Evstatieva LN, Kortenska V. and Handjieva N. 2003. Antioxidant activity screening of extracts from Sideritis species (Labiatae) grown in Bulgaria. Journal of the Science of Food and Agriculture 83, 809-819. doi:10.1002/jsfa.1415
Kursat M, Erecevit P. 2009. The Antimicrobial activities of methanolic extracts of some Lamiaceae members collected from Turkey. Turkish Journal of Science & Technolog, 4 (1) 81-85.
López-Cervantes J, Sánchez-Machado DI, Ríos-Vázquez NJ. 2006. High-performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate. Journal of Chromatography A 1105, 135–139. doi:10.1016/j.chroma.2005.08.010 PMid:16439259
Li HB, Wong CC, Cheng KW, Chen F. 2008. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT 41, 385-390. doi:10.1016/j.lwt.2007.03.011
Liyana-Pathiranan CM, Shahidi F. 2005. Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. Journal of Agricultural and Food Chemistry 53 (243) 3–2440.
Maisuthisakul P, Suttajit M, Pongsawatmanit R. 2007. Assesment of phenolic content and free radicalscavenging capacity of some Thai indigenous plants. Food Chemistry 100, 1409-1418. doi:10.1016/j.foodchem.2005.11.032
Miceli N, Taviano MF, Giuffrida D, Trovato A, Tzakou O, Galati EM. 2005. Anti-inflammatory activity of extract and fractions from Nepeta sibthorpii Bentham. Journal of Ethnopharmacology 97, 261-266. doi:10.1016/j.jep.2004.11.024 PMid:15707763
Miliauskas G, Venskutonis PR, Van Beek TA. 2004. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry 85, 231–237. doi:10.1016/j.foodchem.2003.05.007
Mohamad AA, Khalil AA, El-Beltagi HES. 2010. Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica). Grasas y Aceites 61 (1) 67-75. doi:10.3989/gya.064509
Montoro P, Braca A, Pizza C, Tommasi ND. 2005. Structure-antioxidant activity relationships of flavonoids isolated from different plant species. Food Chemistry 92, 349-355. doi:10.1016/j.foodchem.2004.07.028
Nakiboglu M, Urek R, Kayali H, Tarhan L. 2007. Antioxidant capacities of endemic Sideritis sipylea and Origanum sipyleum from Turkey. Food Chemistry 104, 630-635. doi:10.1016/j.foodchem.2006.12.012
Nostro A, Cannatelli MA, Crisafi G, Alonzo V. 2001. The effect of Nepeta cataria extract on adherence and enzyme production of Staphylococcus aureus. International Journal of Antimicrobial Agents 18, 583–585. doi:10.1016/S0924-8579(01)00452-6
Ozkan G, Sagdic O, Ozcan M, Ozcelik H, Unver A. 2005. Antioxidant and antibacterial activities of Turkish endemic Sideritis extracts. Grasas y Aceites 56, 16−20.
Proestos C, Boziaris IS, Nychas G-JE, Komaitis M. 2006. Analysis of flavonoids and phenolic acids in Grek aromatic plants: Investigation of their antioxidant capacity andantimicrobial activity. Food Chemistry 95 (4) 664-671. doi:10.1016/j.foodchem.2005.01.049
Quilez J, Garcia-Lorda P, Salas-Salvado J. 2003. Potential uses and benefits of phytosterols in diet: present situation and future directions. Clinical Nutrition 22 (4) 343–351. doi:10.1016/S0261-5614(03)00060-8
Sagdic O, Aksoy A, Ozkan G, Ekici L, Albayrak S. 2008. Biological activities of the extracts of two endemic Sideritis species grown in Turkey. Innovative Food Science and Energing Technologies 9, 80-84. doi:10.1016/j.ifset.2007.06.001
Sanchez-Machado DI, Lopez-Hernandez J, Paseiro-Losado P. 2002. High-performance liquid chromatographic determination of a-tocopherol in macroalgae. Journal of Chromatography A 976 (1) 277–284. doi:10.1016/S0021-9673(02)00934-2
Skerget M, Kotnik P, Hadolin M, Hras AR, Simonic M, Knez Z. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry 89, 191–198. doi:10.1016/j.foodchem.2004.02.025
Tepe B, Daferera D, Tepe A, Polissiou M, Sökmen A. 2007. Antioxidant activity of the essential oil and various extracts of Nepeta flavida Hub.-Mor.- from Turkey. Food Chemistry 103, 1358-1364. doi:10.1016/j.foodchem.2006.10.049
Wojdylo A, Oszmianski J, Czemerys R. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry 105, 940-949. doi:10.1016/j.foodchem.2007.04.038
Zu YG, Li CY, Fu YJ. and Zhao C.J. 2006. Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD. Journal of Pharmaceutical and Biomedical Analysis 41, 714–719. doi:10.1016/j.jpba.2005.04.052 PMid:16520013
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