Compuestos biológicos, capacidad atrapadora de radicales libres y actividad antimicrobiana de las semillas de Nepeta italica L. y Sideritis montana L. subsp. montana de Turquía

Autores/as

  • İrfan Emre Firat University, Faculty of Education, Department of Primary Education
  • Murat Kurşat Bitlis Eren University, Faculty of Sciences and Arts, Department of Biology
  • Murat Kurşat Bitlis Eren University, Faculty of Sciences and Arts, Department of Biology
  • Ökkeş Yılmaz Firat University, Faculty of Sciences, Department of Biology
  • Pınar Erecevit Bitlis Eren University, Faculty of Sciences and Arts, Department of Biology

DOI:

https://doi.org/10.3989/gya.033210

Palabras clave:

Ácidos grasos, Actividad antimicrobiana, DPPH radical scavenging actividad, Esteroles, Flavonoides, Nepeta italica L., Sideritis montana L. subsp. Montana, Vitaminas

Resumen


Este estudio ha determinado algunos compuestos biológicos (ácidos grasos, vitaminas liposolubles, esteroles y flavonoides), capacidad atrapadora de radicales libres, y actividades antimicrobianas de las semillas de Nepeta italica L. y Sideritis montana L. subsp. montana. Se encontró que el ácido palmítico (C16:0; 8.54±0.13-3.05±0.04%), ácido oleico (C18:1 n9, 22.41±0.8-18.83±0.1%) y α-linolénico (C18:3 n 3;39.56±0.67-77.04±2.07%) eran mayoritarios en ambas semillas de Nepeta italica L. y Sideritis montana L. subsp. montana. Además, se determinó que tanto Nepeta italica L. como Sideritis montana L. subsp. montana contenían estigmasterol (630.07±1.81μg/g; 80,74±0.71μg/g, respectivamente) y ergosterol (11.01±0.14 μg/g; 161.32±0.63 μg/g respectivamente) y esta última además beta-sitosterol (2.93±0.03μg/g). Además, los resultados muestran que Nepeta italica L. contiene morin (37.79±1.09μg/g), catequina (124.39±2.23μg/g) y naringina (475.96±3.57μg/g) y Sideritis montana L. subsp. montana contiene morin (188.41±2.53μg/g), catequina (64.14±1.86μg/g), naringenina (38.34±1.78 μg/g), como principales flavonoides. También, se determinó que los extractos metanólicos de Nepeta itálica L. y Sideritis montana L. subsp. montana fueron eficaces contra los radicales DPPH. Los resultados obtenidos en el presente estudio mostraron que las vitaminas, los flavonoides y los ácidos grasos de los extractos en las semillas de Nepeta itálica L. y Sideritis montana L. subsp. montana impidieron el crecimiento de los microorganismos utilizados en el ensayo, en diferentes proporciones.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Aboutabl EA, Nassar MI, Elsakhawy FM, Maklad YA, Osman AF, El-Khrisky EAM. 2002. Phytochemical and pharmacological studies on Sideritis taurica Stephen ex Wild. Journal of Ethnopharmacology 82, 177-184. doi:10.1016/S0378-8741(02)00172-1

Ai-li J, Chang-Hai W. 2006. Antioxidant properties of natural components from Salvia plebeia on oxidative stability of ascidian oil. Process Biochemistry 41, 1111-1116. doi:10.1016/j.procbio.2005.12.001

Akpınar N, Akpınar MA, Gorgun S, Dirmenci T, Aktumsek A. 2008. Fatty acid composition of the seeds of five Nepeta species from Turkey. Chemistry of Natural Compounds 44 (1) 90-92. doi:10.1007/s10600-008-0025-9

Azcan N, Ertan A, Demirci B, Bas¸er KHC. 2004. Fatty acid composition of seed oils of twelve Salvia species growing in Turkey. Chemistry of Natural Compounds 40 (3) 218-221. doi:10.1023/B:CONC.0000039127.56323.3e

Barros L, Heleno SA, Carvalho AM, Ferreira ICFR. 2009. Lamiaceae often used in Portuguese folk medicine as a source of powerful antioxidants: Vitamins and phenolics. LWT - Food Science and Technology 1-7.

Basile A, Senatore F, Gargano R, Sorbo S, Del Pezo M, Lavitola A, Ritieni A, Bruno M, Spatuzzi D, Rigano D, Vuotta ML. 2006. Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils. Journal of Ethnopharmacology 107, 240–248. doi:10.1016/j.jep.2006.03.019 PMid:16690235

Bouayed J, Piri K, Rammal H, Dicko A, Desor F, Younos C, Soulimani R. 2007. Comparative evaluation of the antioxidant potential of some Iranian medicinal plants. Food Chemistry 104, 364-368. doi:10.1016/j.foodchem.2006.11.069

Cantino PD. And Sanders, RW. 1986. Subfamilial classification of Labiatae. Syst Bot. 11, 163–185. doi:10.2307/2418955

Christie WW. 1990.Gas Chromatography and lipids. The oily press: Glaskow: UK 573-577.

Collins CH, Lyne PM. 1987. Microbiological Methods Butter Morths & Co (Publishers) Ltd. London, pp. 450.

Davis P.H. 1982. Flora of Turkey and the Aegean Islands. V: 7, Edinburgh Univ. Press., England.

Demo A, Petrakis C, Kefalas P, Boskou D. 1998. Nutrient antioxidants in some herbs and Mediterranean plant leaves. Food Research International 31 (5) 351-354. doi:10.1016/S0963-9969(98)00086-6

Diaz RM, Garcia-Granados A, Moreno E, Parra A, Quevedo-Sarmiento J, Saenz de Buruaga A, Saenz de Buruaga JM. 1988. Studies on the relationship of structure to antimicrobial properties of diterpenoid compound from Sideritis. Planta Medica 54, 301–304. doi:10.1055/s-2006-962439

Erdemoglu N, Turan, NN, Cakıcı I, Bilge Sener B, Aydın A. 2006. Antioxidant Activities of Some Lamiaceae Plant Extracts. Phytother. Res. 20, 9–13. doi:10.1002/ptr.1816 PMid:16397914

Ertan A, Azcan N, Demirci B, Baser KHC. 2001. Fatty acid composition of Sideritis species. Chemistry of Natural Compounds 37 (4) 301-303. doi:10.1023/A:1013775213228

Ezer N, Abbasoglu U. 1996. Antimicrobial activity of essential oils of some Sideritis species growing in Turkey. Fitoterapia 67, 474–475.

Giao MS, Gonzales-Sanjose ML, Rivero-Perez MD, Pereira CI, Pintado ME. and Xavier MF. 2007. Infusions of Portuguese medicinal plants: dependence of final antioxidant capacity and phenol content on extraction features. Journal of the Science of Food and Agriculture 87, 2638-2647. doi:10.1002/jsfa.3023 PMid:20836172

Grzegorczyk I, Matkowski A, Wysokinska H, 2007. Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chemistry 104, 536–54. doi:10.1016/j.foodchem.2006.12.003

Güvenc A, Okada Y, Ku.peli Akkol E, Duman H, Okuyama T, Çalıs I. 2010. Investigations of anti-inflammatory, antinociceptive, antioxidant and aldose reductase inhibitory activities of phenolic compounds from Sideritis brevibracteata. Food Chemsitry 118, 686–692. doi:10.1016/j.foodchem.2009.05.034

Hara A, Radin NS. 1978. Lipid extraction of tissues with a low-toxicity solvent. Anal.Biochem. 90 (1) 420-426. doi:10.1016/0003-2697(78)90046-5

Hernandez-Perez M, Rabanal RM. 2002. Evaluation of the antinflammatory and analgesic activity of Sideritis canariensis var. pannosa in mice. Journal of Ethnopharmacology 81, 43-47. doi:10.1016/S0378-8741(02)00033-8

Hussain J, Ulah F, Hussain H, Hussain S.T. and Shah M.R. 2005. Nepetolide:A new diterpene from Nepeta suavis. Z. Naturforsch. 63 (b) 591-594.

Kiliç T, Dirmenci T, Gören AC. 2007. Fatty acids of seeds of some species of Nepeta L. Natural Product Research 21 (5) 465-468. doi:10.1080/14786410601129473 PMid:17487620

Klimek B. and Modnicki D. 2005. Terpenoids and sterols from Nepeta cataria L. var. citriodora (Lamniaceae). Acta Pol Pharm. 62 (3) 231-235. PMid:16193817

Koleva II, Linssen JPH, van Beek TA, Evstatieva LN, Kortenska V. and Handjieva N. 2003. Antioxidant activity screening of extracts from Sideritis species (Labiatae) grown in Bulgaria. Journal of the Science of Food and Agriculture 83, 809-819. doi:10.1002/jsfa.1415

Kursat M, Erecevit P. 2009. The Antimicrobial activities of methanolic extracts of some Lamiaceae members collected from Turkey. Turkish Journal of Science & Technolog, 4 (1) 81-85.

López-Cervantes J, Sánchez-Machado DI, Ríos-Vázquez NJ. 2006. High-performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate. Journal of Chromatography A 1105, 135–139. doi:10.1016/j.chroma.2005.08.010 PMid:16439259

Li HB, Wong CC, Cheng KW, Chen F. 2008. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT 41, 385-390. doi:10.1016/j.lwt.2007.03.011

Liyana-Pathiranan CM, Shahidi F. 2005. Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. Journal of Agricultural and Food Chemistry 53 (243) 3–2440.

Maisuthisakul P, Suttajit M, Pongsawatmanit R. 2007. Assesment of phenolic content and free radicalscavenging capacity of some Thai indigenous plants. Food Chemistry 100, 1409-1418. doi:10.1016/j.foodchem.2005.11.032

Miceli N, Taviano MF, Giuffrida D, Trovato A, Tzakou O, Galati EM. 2005. Anti-inflammatory activity of extract and fractions from Nepeta sibthorpii Bentham. Journal of Ethnopharmacology 97, 261-266. doi:10.1016/j.jep.2004.11.024 PMid:15707763

Miliauskas G, Venskutonis PR, Van Beek TA. 2004. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry 85, 231–237. doi:10.1016/j.foodchem.2003.05.007

Mohamad AA, Khalil AA, El-Beltagi HES. 2010. Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica). Grasas y Aceites 61 (1) 67-75. doi:10.3989/gya.064509

Montoro P, Braca A, Pizza C, Tommasi ND. 2005. Structure-antioxidant activity relationships of flavonoids isolated from different plant species. Food Chemistry 92, 349-355. doi:10.1016/j.foodchem.2004.07.028

Nakiboglu M, Urek R, Kayali H, Tarhan L. 2007. Antioxidant capacities of endemic Sideritis sipylea and Origanum sipyleum from Turkey. Food Chemistry 104, 630-635. doi:10.1016/j.foodchem.2006.12.012

Nostro A, Cannatelli MA, Crisafi G, Alonzo V. 2001. The effect of Nepeta cataria extract on adherence and enzyme production of Staphylococcus aureus. International Journal of Antimicrobial Agents 18, 583–585. doi:10.1016/S0924-8579(01)00452-6

Ozkan G, Sagdic O, Ozcan M, Ozcelik H, Unver A. 2005. Antioxidant and antibacterial activities of Turkish endemic Sideritis extracts. Grasas y Aceites 56, 16−20.

Proestos C, Boziaris IS, Nychas G-JE, Komaitis M. 2006. Analysis of flavonoids and phenolic acids in Grek aromatic plants: Investigation of their antioxidant capacity andantimicrobial activity. Food Chemistry 95 (4) 664-671. doi:10.1016/j.foodchem.2005.01.049

Quilez J, Garcia-Lorda P, Salas-Salvado J. 2003. Potential uses and benefits of phytosterols in diet: present situation and future directions. Clinical Nutrition 22 (4) 343–351. doi:10.1016/S0261-5614(03)00060-8

Sagdic O, Aksoy A, Ozkan G, Ekici L, Albayrak S. 2008. Biological activities of the extracts of two endemic Sideritis species grown in Turkey. Innovative Food Science and Energing Technologies 9, 80-84. doi:10.1016/j.ifset.2007.06.001

Sanchez-Machado DI, Lopez-Hernandez J, Paseiro-Losado P. 2002. High-performance liquid chromatographic determination of a-tocopherol in macroalgae. Journal of Chromatography A 976 (1) 277–284. doi:10.1016/S0021-9673(02)00934-2

Skerget M, Kotnik P, Hadolin M, Hras AR, Simonic M, Knez Z. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry 89, 191–198. doi:10.1016/j.foodchem.2004.02.025

Tepe B, Daferera D, Tepe A, Polissiou M, Sökmen A. 2007. Antioxidant activity of the essential oil and various extracts of Nepeta flavida Hub.-Mor.- from Turkey. Food Chemistry 103, 1358-1364. doi:10.1016/j.foodchem.2006.10.049

Wojdylo A, Oszmianski J, Czemerys R. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry 105, 940-949. doi:10.1016/j.foodchem.2007.04.038

Zu YG, Li CY, Fu YJ. and Zhao C.J. 2006. Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD. Journal of Pharmaceutical and Biomedical Analysis 41, 714–719. doi:10.1016/j.jpba.2005.04.052 PMid:16520013

Descargas

Publicado

2011-03-30

Cómo citar

1.
Emre İrfan, Kurşat M, Kurşat M, Yılmaz Ökkeş, Erecevit P. Compuestos biológicos, capacidad atrapadora de radicales libres y actividad antimicrobiana de las semillas de Nepeta italica L. y Sideritis montana L. subsp. montana de Turquía. Grasas aceites [Internet]. 30 de marzo de 2011 [citado 4 de mayo de 2025];62(1):68-75. Disponible en: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1300

Número

Sección

Investigación