Evaluation of different green technological approaches for the extraction of oil from grape seeds: A comparative study
DOI:
https://doi.org/10.3989/gya.1200232.2173Keywords:
Fatty acid, Grape seed oil, Para-cymene, Supercritical carbon-dioxideAbstract
The present study aimed to assess the impact of eco-friendly approaches, including supercritical CO2 (SCO), supercritical CO2+ethanol (SCE), para-cymene (PC), and the cold-press (CP) method on the quality characteristics of grape seed oil (GSO). The results revealed that the PC approach produced the maximum oil yield of 19.46 %, followed by SCE (18.61 %), Hexane (HX) (18.03 %), SCO (16.47 %), and CP (11.01 %). The fatty acid profile and antioxidant activity of SCE oil were superior to all other tested approaches. The total phenolic content (TPC) in the extracted oils ranged from 109.77 mg GAE·kg–1 to 132.01 mg GAE·kg–1, with SCE having the highest TPC and HX having the lowest TPC. PC, SCO, SCE, and CP extracted oils exhibited high total tocopherol contents (TTC) of 233.61, 257.01, 264.26 and 251.89 mg·kg–1 as compared to 214.13 mg·kg–1 in hexane, respectively. The overall findings demonstrate that adopting environmentally sustainable solvents and extraction methods could serve as a preferable alternative to conventional approaches without compromising the quality characteristics of the extracted grape seed oil.
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Ahamad J, Naquvi KJ, Uthirapathy S, Naim MJ, Majedi S. 2024. Monounsaturated and polyunsaturated fatty acids. In: Bioactive Compounds of Edible Oils and Fats. pp. 71-87. CRC Press. ISBN 9781003450719.
Al Juhaimi F, Özcan MM, Ghafoor K, Babiker EE, Hussain S. 2018. Comparison of cold-pressing and soxhlet extraction systems for bioactive compounds, antioxidant properties, polyphenols, fatty acids and tocopherols in eight nut oils. J. Food Sci. Technol. 55, 3163-3173.
Anusuya N, Gomathi R, Tharani J, Murugesan GS. 2013. Impact of polyphenols from banana pseudostem on sunflower oil stability. Food Sci. Biotechnol. 22, 773-780.
AOAC 1990. Official Methods of Analysis of AOAC International, Association of Official Analytical Chemists, 15th Edn. AOAC International, Washington DC, USA.
Bail S, Stuebiger G, Krist S, Unterweger H, Buchbauer G. 2008. Characterisation of various grape seed oils by volatile compounds, triacylglycerol composition, total phenols and antioxidant capacity. Food Chem. 108 (3), 1122-1132.
Baydar NG, Akkurt M. 2001. Oil content and oil quality properties of some grape seeds. Turk J. Agric. Forest. 25 (3), 163-168. https://journals.tubitak.gov.tr/agriculture/vol25/iss3/3.
Baydar NG, Özkan G, Çetin ES. 2007. Characterization of grape seed and pomace oil extracts. Grasas Aceites 58 (1), 29-33.
Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem. 239 (1), 70-76.
Beveridge TH, Girard B, Kopp T, Drover JC. 2005. Yield and composition of grape seed oils extracted by supercritical carbon dioxide and petroleum ether: varietal effects. J Agric. Food Chem. 53 (5), 1799-1804.
Chemat F, Vian MA. 2014 (Eds.). Alternative solvents for natural products extraction. Springer Berlin Heidelberg.
Coelho JP, Filipe RM, Robalo MP, Boyadzhieva S, Cholakov GS, Stateva RP. 2020. Supercritical CO2 extraction of spent coffee grounds. Influence of co-solvents and characterization of the extracts. J. Supercrit. Fluids 161, 104825.
Da Silva TL, Bernardo EC, Nobre B, Mendes RL, Reis A. 2008. Extraction of victoria and red globe grape seed oils using supercritical carbon dioxide with and without ethanol. J Food Lipids. 15 (3), 356-369.
Dąbrowski G, Skrajda M, Tańska M, Roszkowska B. 2016. Influence of Different Storage Conditions on Rapeseed Oils Quality. Am. J. Exp. Agric. 10 (5), 1-12.
Di Stefano V, Bongiorno D, Buzzanca C, Indelicato S, Santini A, Lucarini M, Fabbrizio A, Mauro M, Vazzana M, Arizza V, Durazzo A. 2021. Fatty acids and triacylglycerols profiles from Sicilian (cold pressed vs. soxhlet) grape seed oils. Sustainability. 13 (23), 13038.
Downey MO, Harvey JS, Robinson SP. 2003. Analysis of tannins in seeds and skins of Shiraz grapes throughout berry development. Aust. J. Grape Wine Res. 9 (1), 15-27.
Duba K, Fiori L. 2019. Supercritical CO2 extraction of grape seeds oil: scale-up and economic analysis. Int. J. Food Sci. Technol. 54 (4), 1306-1312.
Dubois V, Breton S, Linder M, Fanni J, Parmentier M. 2007. Fatty acid profiles of 80 vegetable oils with regard to their nutritional potential. Eur. J. Lipid Sci. Technol. 109 (7), 710-732.
Elisia I, Yeung M, Kowalski S, Shyp T, Tee J, Hollman S, Wong A, King J, Dyer R, Sorensen HP, Krystal G. 2024. A ketogenic diet rich in fish oil is superior to other fats in preventing NNK-induced lung cancer in A/J mice. Sci. Rep. 14 (1), 5610.
Fatemi SH, Hammond EG. 1980. Analysis of oleate, linoleate and linolenate hydroperoxides in oxidized ester mixtures. Lipids 15 (5), 379-385.
Fernandes L, Casal S, Cruz R, Pereira JA, Ramalhosa E. 2013. Seed oils of ten traditional Portuguese grape varieties with interesting chemical and antioxidant properties. Food Res. Int. 50 (1), 161-166.
Haiyan Z, Bedgood Jr DR, Bishop AG, Prenzler PD, Robards K. 2007. Endogenous biophenol, fatty acid and volatile profiles of selected oils. Food Chem. 100 (4), 1544-1551.
Ichihara KI, Fukubayashi Y. 2010. Preparation of fatty acid methyl esters for gas-liquid chromatography [S]. J. Lipid Res. 51(3), 635-640.
Kumar SJ, Prasad SR, Banerjee R, Agarwal DK, Kulkarni KS, Ramesh KV. 2017. Green solvents and technologies for oil extraction from oilseeds. Chem. Cent. J. 11, 1-7.
Lee JC, Kim HR, Kim J, Jang YS. 2002. Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. saboten. J. Agric. Food Chem. 50 (22), 6490-6496.
Li H, Fu X, Deng G, David A, Huang L. 2020. Extraction of oil from grape seeds (Vitis vinifera L.) using recyclable CO2-expanded ethanol. Chem. Eng. Process.: Process Intensif. 157, 108147.
Maghsoudlou E, Esmaeilzadeh Kenari R, Raftani Amiri Z. 2017. Evaluation of antioxidant activity of fig (Ficus carica) pulp and skin extract and its application in enhancing oxidative stability of canola oil. J. Food Process. Preserv. 41 (4), e13077.
Maszewska M, Florowska A, Matysiak K, Marciniak-Łukasiak K, Dłużewska E. 2018. The study of palm and rapeseed oil stability during frying. J. Appl. Bot. Food Qual. 91, 103-108.
Rababah TM, Ereifej KI, Al-Mahasneh MA, Ismaeal K, Hidar AG, Yang W. 2008. Total phenolics, antioxidant activities, and anthocyanins of different grape seed cultivars grown in Jordan. Int. J. Food Prop. 11 (2), 472-479.
Redondo-Cuevas L, Castellano G, Torrens F, Raikos V. 2018. Revealing the relationship between vegetable oil composition and oxidative stability: A multifactorial approach. J. Food Compos. Anal. 66, 221-229.
Rombaut N, Savoire R, Thomasset B, Bélliard T, Castello J, Van Hecke É, Lanoisellé JL. 2014. Grape seed oil extraction: Interest of supercritical fluid extraction and gas-assisted mechanical extraction for enhancing polyphenol co-extraction in oil. C. R. Chim. 17 (3), 284-292.
Sabir A, Unver A, Kara Z. 2012. The fatty acid and tocopherol constituents of the seed oil extracted from 21 grape varieties (Vitis spp.). J. Sci. Food Agric. 92 (9), 1982-1987.
Spika MJ, Kraljic K, Koprivnjak O, Skevin D, Anetic M, Katalinic M. 2015. Effect of agronomical factors and storage conditions on the tocopherol content of Oblica and Leccino virgin olive oils. J. Am. Oil Chem. Soc. 92 (9), 1293.
Vieira DS, Menezes M, Gonçalves G, Mukai H, Lenzi EK, Pereira NC, Fernandes PRG. 2015. Temperature dependence of refractive index and of electrical impedance of grape seed (Vitis vinifera, Vitis labrusca) oils extracted by Soxhlet and mechanical pressing. Grasas Aceites, 66 (3), e083.
Wada B, Gordon R, Yagiz Y, Gu L. 2018. Comparing the oil extraction and refining methods for muscadine grape seeds of Noble and Carlos cultivar. Eur. J. Lipid Sci. Technol. 120 (9), 1800166.
Yu J, Ahmedna M. 2013. Functional components of grape pomace: Their composition, biological properties and potential applications. Int. J. Food Sci. 48 (2), 221-237.
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