Análisis de múltiples micotoxinas en aceite de maíz comercial de Indonesia mediante cromatografía de alta resoluciónespectrometría de masas (UHPLC-HRMS)
DOI:
https://doi.org/10.3989/gya.0757241.2208Palabras clave:
acetonitrilo, dilución isotópica, efectos de matriz, matriz de aceite, Q-Orbitrap, UHPLC QE-MSResumen
La detección de micotoxinas en el aceite de maíz plantea importantes retos técnicos debido a su compleja matriz, que puede perturbar tanto los procesos de extracción como de análisis. El objetivo de esta investigación era establecer un método robusto para detectar micotoxinas en el aceite de maíz con precisión y eficacia. Se utilizó aceite de maíz comercial procedente de Indonesia, empleando un pretratamiento de la muestra mediante extracción complementaria líquido-líquido. Se utilizó UHPLC-HRMS para identificar múltiples micotoxinas. La investigación demostró que la extracción complementaria líquido-líquido mejoraba en gran medida la recuperación de micotoxinas (95% - 106%). La validación del método incluyó la evaluación de parámetros como la linealidad (R2>0,99), la recuperación (RSD < 11,7%), la precisión intradiaria e interdiaria (RSD < 16%), LOD < 0,03 ppb y LOQ < 0,40 ppb. La precisión del método se confirmó comparando los resultados con LC-ID-MS/MS y materiales de referencia certificados (CRM). El análisis de muestras comerciales de maíz procedentes de Indonesia reveló niveles elevados de micotoxinas tanto en piensos para animales como en maíz destinado al consumo humano, con niveles de fumonisina B1 (FB1) de hasta 3214 ppb.
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