Aislamiento e identificación de bacterias lácticas durante la fermentación espontánea de aceitunas de mesa obtenidas mediante desamargado con bicarbonato sódico
DOI:
https://doi.org/10.3989/gya.0105251.2281Palabras clave:
Aceituna, Aceituna de mesa, Bacterias lácticas, Bicarbonato sódico, Desamargor, FermentaciónResumen
Las aceitunas de mesa obtienen su sabor y aroma únicos como resultado de la fermentación, que se aplica junto con el proceso de desamargado. En este estudio, se utilizaron aceitunas verdes Domat y negras Gemlik, que son cultivares turcos muy comunes, para la producción de aceitunas de mesa, el desamargado se realizó con bicarbonato de sodio (NaHCO₃). La fermentación se controló mediante pH, acidez valorable, azúcares reductores, contenido total de fenoles y análisis microbiológicos durante un período de fermentación de 120 días. Las bacterias del ácido láctico (BAL) se identificaron mediante API 50 CHL y se evaluó el efecto del NaHCO₃ en el perfil de BAL. Se determinó que había 3 especies de BAL: Lactobacillus plantarum (6 aislados, 12%), Lactobacillus pentosus (27 aislados, 54%) y Lactococcus mesenteroides ssp. mesenteroides (17 aislados, 34%). La mayoría de los aislados identificados como Lactobacillus se identificaron como L. pentosus. La especie L. plantarum se aisló únicamente de aceitunas verdes de mesa y no se encontró en aislados de muestras de aceitunas negras de mesa.
Descargas
Citas
Abriouel H, Benomar N, Cobo A, Caballero N, Fuentes MÁ, Pérez-Pulido R, Gálvez A. 2012. Characterization of lactic acid bacteria from naturally-fermented Manzanilla Aloreña green table olives. Food Microbiol. 32 (2), 308-16. https://doi.org/10.1016/j.fm.2012.07.006 PMid:22986194
Anagnostopoulos DA, Kamilari E, Tsaltas D. 2020. Evolution of bacterial communities, physicochemical changes and sensorial attributes of natural whole and cracked Picual table olives during spontaneous and inoculated fermentation. Front. Microbiolol. 11, 1128. https://doi.org/10.3389/fmicb.2020.01128 PMid:32547528 PMCid:PMC7273852
Aprile A, Negro C, Sabella E, Luvisi A, Nicolì F, Nutricati E, Vergine M, Miceli A, Blando F, De Bellis L. 2019. Antioxidant Activity and Anthocyanin Contents in Olives (cv Cellina di Nardò) during Ripening and after Fermentation. Antioxidants 8, 138. https://doi.org/10.3390/antiox8050138 PMid:31109100 PMCid:PMC6562514
Argyri K, Doulgeraki AI, Manthou E, Grounta A, Argyri AA, Nychas GJE, Tassou CC. 2020. Microbial diversity of fermented Greek table olives of Halkidiki and Konservolia varieties from different regions as revealed by metagenomic analysis. Microorganisms 8 (8), 1241. https://doi.org/10.3390/microorganisms8081241 PMid:32824085 PMCid:PMC7464643
Bautista-Gallego J, Arroyo-López FN, Rantsiou K, Jiménez-Díaz R, Garrido-Fernández A, Cocolin L. 2013. Screening of lactic acid bacteria isolated from fermented table olives with probiotic potential. Food Res. Int. 50 (1), 135-42. https://doi.org/10.1016/j.foodres.2012.10.004
Ben Othman N, Roblain D, Chammen N, Thonart P, Hamdi M. 2009. Antioxidant phenolic compounds loss during the fermentation of Chétoui olives. Food Chem. 116 (3), 662-669. https://doi.org/10.1016/j.foodchem.2009.02.084
Benítez-Cabello A, Romero-Gil V, Rodríguez-Gómez F, Garrido-Fernández A, Jiménez-Díaz R, Arroyo-López FN. 2015. Evaluation and identification of poly-microbial biofilms on natural green Gordal table olives. A. Van Leeuw. J. Microb. 108, 597-610. https://doi.org/10.1007/s10482-015-0515-2 PMid:26115883
Borcakli M, Özay G, Alperden I, Özsan E, Erdek Y. 1993. Changes in chemical and microbiological composition of two varieties of olive during fermentation. Grasas Aceites 44 (4-5), 253-8. https://doi.org/10.3989/gya.1993.v44.i4-5.1075
Boskou G. 2010. Antioxidant Capacity and Phenolic Profile of Table Olives from the Greek Market. Olives and Olive Oil in Health and Disease Prevention, 925-934. https://doi.org/10.1016/B978-0-12-374420-3.00099-1
Campaniello D, Bevilacqua A, Damato D, Corbo MR, Altieri C, Sinigaglia M. 2005. Microbial characterization of table olives processed according to Spanish and natural styles. Food. Technol. Biotechnol. 43 (3) 289-294. https://hrcak.srce.hr/110552
Comunian R, Ferrocino I, Paba A, Daga E, Campus M, Di Salvo R, Cauli E, Piras F, Zurru R, Cocolin L. 2017. Evolution of microbiota during spontaneous and inoculated Tonda di Cagliari table olives fermentation and impact on sensory characteristics. LWT-Food Sci. Technol. 84, 64-72. https://doi.org/10.1016/j.lwt.2017.05.039
Cocolin L, Alessandria V, Botta C, Gorra R, De Filippis F, Ercolini D, Rantsiou K. 2013. NaOH-debittering induces changes in bacterial ecology during table olives fermentation. PLoS One. 8(7), e69074. https://doi.org/10.1371/journal.pone.0069074 PMid:23935928 PMCid:PMC3729808
Coimbra-Gomes J, Reis PJ, Tavares TG, Malcata FX, Macedo AC. 2022. Study of lactic acid bacteria biodiversity in fermented Cobrançosa table olives to determine their probiotic potential. Foods 11 (19), 3050. https://doi.org/10.3390/foods11193050 PMid:36230126 PMCid:PMC9563300
Corsetti A, Perpetuini G, Schirone M, Tofalo R, Suzzi G. 2012. Application of starter cultures to table olive fermentation: an overview on the experimental studies. Front. Microbiol. 3, 248. https://doi.org/10.3389/fmicb.2012.00248
Doulgeraki AI, Pramateftaki P, Argyri AA, Nychas GJ, Tassou CC, Panagou EZ. 2013. Molecular characterization of lactic acid bacteria isolated from industrially fermented Greek table olives. LWT-Food Sci. Technol. 50(1), 353-6. https://doi.org/10.1016/j.lwt.2012.07.003
Garrido-Fernández A, Fernández-Diez MJ, AdamsMR. 1997. Table olives: production and processing, Chapman & Hall (Eds.). London, UK
Hurtado A, Othman NB, Chammem N, Hamdi M, Ferrer S, Reguant C, Bordons A, Rozès N. 2011. Characterization of Lactobacillus isolates from fermented olives and their bacteriocin gene profiles. Food Microbiol. 28(8), 1514-8. https://doi.org/10.1016/j.fm.2011.07.010 PMid:21925038
Idoui T, Boudjerda J, Leghouchi E, Karam NE. 2009. Naturally fermented Jijelian black olives: microbiological characteristics and isolation of lactic acid bacteria. Grasas Aceites 60 (5), 516-520. https://doi.org/10.3989/gya.043009
Kacem M, Zadi-Karam H, Nour-Eddine K. 2005. Isolation of lactic acid bacteria from naturally fermented Algerian olives. J. King Saud Univ. Sci. 18, 89-98.
Kiai H, Hafidi A. 2014. Chemical composition changes in four green olive cultivars during spontaneous fermentation. LWT-Food Sci. Technol. 57, 663-670. https://doi.org/10.1016/j.lwt.2014.02.011
Lucena-Padrós H, Caballero-Guerrero B, Maldonado-Barragán A, Ruiz-Barba JL. 2014. Microbial diversity and dynamics of Spanish-style green table-olive fermentations in large manufacturing companies through culture-dependent techniques. Food Microbiol. 42, 154-65. https://doi.org/10.1016/j.fm.2014.03.020 PMid:24929732
Mitsopoulos G, Hagidimitriou M, Papageorgiou V, Komaitis M. 2010. Total phenolic content, phenolic profile and antioxidant activity in leaves and drupes of Greek olive cultivars. InXXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): Olive Trends Symposium-From the 924 2010 Aug 22 (pp. 425-430). https://doi.org/10.17660/ActaHortic.2011.924.54
Mourad K, Halima ZK, Nour-Eddine K. 2004. Isolation of lactic acid bacteria for its possible use in the fermentation of green Algerian olives. Grasas Aceites 55(4), 385-93. https://doi.org/10.3989/gya.2004.v55.i4.205
Portilha-Cunha FM, Angela C, Macedo AC, Malcata FX. 2020. A review on adventitious lactic acid bacteria from table olives. Foods 9(7), 948. https://doi.org/10.3390/foods9070948 PMid:32709144 PMCid:PMC7404733
Reis PJM, Tavares TG, Rocha JM, Malcata FX, Macedo AC. 2022. Cobrançosa table olives: characterization of processing method and lactic acid bacteria profile throughout spontaneous fermentation. Appl. Sci. 12 (19), 9738. https://doi.org/10.3390/app12199738
Ronda A, Martín‐Lara MÁ, Blázquez G, Bachs NM, Calero M. 2014. Copper biosorption in the presence of lead onto olive stone and pine bark in batch and continuous systems. Environ. Prog. Sustain. 33(1), 192-204. https://doi.org/10.1002/ep.11780
Ruiz-Barba JL, Cathcart DP, Warner PJ, Jiménez-Diaz R. 1994. Use of Lactobacillus plantarum LPCO10, abacteriocin producer as starter culture in Spanish-style green olive fermentations. Appl. Environ. Microb. 60, 2059-2064. https://doi.org/10.1128/aem.60.6.2059-2064.1994 PMid:16349291 PMCid:PMC201601
Ryan D, Robards K, Lavee S. 1999. Changes in phenolic content of olive during maturation. Int. J. Food Sci. Tech. 34 (3), 265-74. https://doi.org/10.1046/j.1365-2621.1999.00261.x
Sevim D, Tuncay O, Koseoglu O. 2013. The effect of olive leaf addition on antioxidant content and antioxidant activity of "Memecik" olive oils at two maturity stages. J Am. Oil Chem. Soc. 90, 1359-69. https://doi.org/10.1007/s11746-013-2282-4
Tufariello M, Mita G, Bleve G. 2016. Biotechnology can improve a traditional product as table olives. Products from Olive Tree. 235-60. https://doi.org/10.5772/64687
Tzamourani AP, Di Napoli E, Paramithiotis S, Economou-Petrovits G, Panagiotidis S, Panagou EZ. 2021. Microbiological and physicochemical characterisation of green table olives of Halkidiki and Conservolea varieties processed by the Spanish method on industrial scale. Int. J. Food Sci. Tech. 56(8), 3845-57. https://doi.org/10.1111/ijfs.15000
Van den Berg DJ, Smits A, Pot B, Ledeboer AM, Kersters K, Verbake JM, Verrips CT. 1993. Isolation, screening and identification of lactic acid bacteria from traditional food fermentation processes and culture collections. Food Biotechnol. 7(3), 189-205. https://doi.org/10.1080/08905439309549857
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2025 Consejo Superior de Investigaciones Científicas (CSIC)

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
© CSIC. Los originales publicados en las ediciones impresa y electrónica de esta Revista son propiedad del Consejo Superior de Investigaciones Científicas, siendo necesario citar la procedencia en cualquier reproducción parcial o total.
Salvo indicación contraria, todos los contenidos de la edición electrónica se distribuyen bajo una licencia de uso y distribución “Creative Commons Reconocimiento 4.0 Internacional ” (CC BY 4.0). Consulte la versión informativa y el texto legal de la licencia. Esta circunstancia ha de hacerse constar expresamente de esta forma cuando sea necesario.
No se autoriza el depósito en repositorios, páginas web personales o similares de cualquier otra versión distinta a la publicada por el editor.
Datos de los fondos
Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
Números de la subvención 221O048








