Grasas y Aceites, Vol 71, No 1 (2020)

Enzyme-assisted three-phase partitioning: An efficient alternative for oil extraction from Sesame (Sesamum indicum L.)


https://doi.org/10.3989/gya.1060182

P. Juvvi
Department of Technology Scale-up, CSIR-Central Food Technological Research Institute, India
orcid https://orcid.org/0000-0003-3709-156X

S. Debnath
Department of Technology Scale-up, CSIR-Central Food Technological Research Institute, India
orcid https://orcid.org/0000-0002-5160-3093

Abstract


Three-phase partitioning (TPP) was explored for oil extraction from Sesamum indicum L. seeds. The process parameters, namely the salt concentration, slurry/t-butanol ratio and system pH were standardized. The optimum conditions for maximum oil recovery using TPP were an ammonium sulphate concentration of 40% (w/v), slurry/t-butanol ratio of 1:1 (v/v) and system pH of 5.0. The powdered seeds were subjected to enzyme-assisted three-phase partitioning (EATPP) which was pre-treated with pectinase, protease and a mixture of ɑ-amylase and amylo-glucosidase (1:1 ratio) followed by TPP (as standardized conditions) and its efficacy in recovering oil was compared with TPP and solvent extraction (SE). Out of all the enzymes studied, EATPP with pectinase resulted in the highest oil recovery (86.12%), which was higher than that of TPP (78.24%). The free fatty acids, saponification value and peroxide values were observed to be lower in the case of TPP and EATPP when compared to SE, indicating better oil quality.

Keywords


Cell wall hydrolysis; Enzyme-assisted three-phase partitioning (EATPP): Solvent extraction (SE); Green extraction method; Sesame oil

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References


Anwar F, Zreen Z, Sultana B, Jamil A. 2013. Enzyme-aided cold pressing of flaxseed (Limum usitatissimum L.): Enhancement in yield, quality and phenolics of oil. Grasas Aceites 64, 463–471.

AOAC. 2002. Official methods and analysis, 17th edn. Association of Official Analytical Chemists, Washington, DC.

AOAC. 2005. Official methods of analysis of 18th edition, Association of Official Analytical Chemistry. Virginia, USA, Vol. II, Method no 41.1.1, 41.1.15 and 41.1.21 P.7.

AOCS. 2000. Official methods and recommended practices, 4th edn. Am. Oil Chem. Soc., Champaign, USA.

AOCS. 2002. Official methods and recommended practices of the AOCS, Ca 5a-40, Cd 8-53, Ce 1- 62, Champaign, USA.

Che Man YB, Suhardiyono, Asbi AB, Azudin MN, Wei LS. 1996. Aqueous enzymatic extraction of coconut oil. J. Am. Oil Chem. Soc. 73, 683–686.

Crapisi A, Lante A, Pasini, G, Spettoli P. 1993. Enhanced microbial cell lysis by the use of lysozyme immobilized on different carriers. Process Biochem. 28, 17–21.

Debnath S, Raghavarao KSMS, Lokesh BR. 2011. Hydrodynamic, thermo-analytical and molecular structural investigations of enzyme interesterified oil and its thermos-oxidative stability by thermogravimetric analysis. J. Food Eng. 105, 671–679.

Dutta R, Sarkar U, Mukherjee A. 2015. Process optimization for the extraction of oil from Crotalaria juncea using three-phase partitioning. Ind. Crops Prod. 71, 89–96.

Elleuch M, Bedigian D, Besbes S, Blecket C, Attia H. 2012. Dietary fibre characteristics and antioxidant activity of Sesame seed coats (testae). Int. J. Food Proper. 15, 25–37.

Ferreira-Dias S, Valente DG, Abreu JM. 2003. Comparison between ethanol and hexane for oil extraction from Quercus suber L. fruits. Grasas Aceites 54, 378–383.

Henna Lu FS, Tan PP. 2009. A Comparative study of storage stability in virgin coconut oil and extra virgin olive oil upon thermal treatment. Inter. Food Res. J. 16, 343–354.

Huang AHC. 1994. Structure of plant seed oil bodies. Curr. Opin. Struct. Biol. 4, 494–498.

Ketnawa S, Benjakul S, Martinez-Alvarez O, Rawdkuen S. 2014. Three-phase partitioning and proteins hydrolysis patterns of alkaline proteases derived from fish viscera. Sep. Purif. Technol. 132, 174–18.

Kurmudle NN, Bankar SB, Bajaj IB, Bule MV, Singhal RS. 2011. Enzyme-assisted three-phase partitioning: A novel approach for extraction of turmeric oleoresin. Process Biochem. 46, 423–426.

Latif S, Anwar F, Ashraf M. 2007. Characterization of enzyme-assisted cold pressed cottonseed oil. J. Food Lipids 14, 424–436.

Latif S, Diosady LL, Anwar F. 2008. Enzyme-assisted aqueous extraction of oil and protein from canola (Brassica napus L.) seeds. Eur. J. Lipid Sci. Technol. 110, 887–892.

Latif S, Anwar F. 2009. Physico-chemical studies of hemp (Cannabis sativa) seed oil using enzyme-assisted cold pressing. Eur. J. Lipid Sci. Technol. 111, 1042–1048.

Latif S, Anwar F. 2011. Aqueous enzymatic Sesame oil and protein extraction. Food Chem. 125, 679–684.

Li Z, Li Y, Zhang X, Tan T. 2015. Lipid extraction from non-broken and high water content microalgae Chlorella spp. by three-phase partitioning. Algal Res. 10, 218–223.

Mbaebie B, Omosun G, Uti A, Oyedemi S. 2010. Chemical composition of Sesamum indicum L. (Sesame) grown in south-eastern Nigeria and the physicochemical properties of the seed oil. Seed Sci. Biotechnol. 4, 69–72.

Mondal A. 2015. Sonication assisted three-phase partitioning for the extraction of palmitic acid and elaidic acid from Cassia sophera Linn. Sep. Sci. Technol. 50, 1999-2003.

Mushtaq M, Sultana B, Anwar F, Rizvi SSH. 2015. Enzyme-assisted supercritical fluid extraction of phenolic antioxidants from pomegranate peel. J. Supercritical Fluids 104, 122–131.

Namiki M. 2007. Nutraceuticals functions of Sesame: A review. Critic. Rev. Food Sci. Nutr. 47, 651–673.

Namiki, M. 1995. The chemistry and physiological functions of Sesame. Food Rev. Int. 11, 281–329.

Panadare DC, Rathod VK. 2017. Three-phase partitioning of oil: A review. Trends Food Sci. Technol. 68, 145–151.

Raja Rajan RG, Gopala Krishna AG. 2014. A simple method for purification of deodorizer distillate from Indian rice (Oryza Sativa) bran oil and preparation of phytosterols. Grasas Aceites 65, 1–7.

Reena MB, Reddy SRY, Lokesh BR. 2009. Changes in triacylglycerol molecular species and thermal properties of blended and interesterified mixtures of coconut oil or palm oil with ricebran oil or Sesame oil. Eur. J. Lipid Sci. Technol. 111, 346–357.

Reshma MV, Balachandran C, Arumughan C, Sarita SS. 2010. Extraction, separation and characterisation of Sesame oil lignin for nutraceutical applications. Food Chem. 120, 1041–1046.

Rosenthal A, Pyle DL, Niranjan K. 1996. Aqueous and enzymatic processes for edible oil extraction. Enzyme Microb. Technol. 19, 402–420.

Rossell JB, Prichard JLR. 1991. Analysis of oilseeds, fats and fatty foods. London: Elsevier Science Publishers Ltd.

Ruchi G, Sharma A, Khare SK, Gupta MN. 2007. A novel process for extraction of edible oils enzyme assisted three-phase partitioning (EATPP). Bioresour. Technol. 98, 696–699.

Sant’Anna BPM, Freitas SP, Coelho MAZ. 2003. Enzymatic aqueous technology for simultaneous coconut protein and oil extraction. Grasas Aceites 54, 77–80.

Shah S, Sharma A, Gupta MN. 2004. Extraction of oil from Jatropha curcas L. seed kernels by enzyme assisted three-phase partitioning. Ind. Crops Prod. 20, 275–279. https://doi.org/10.1016/j.indcrop.2003.10.010

Sharma A, Gupta MN. 2001a. Purification of pectinases by three-phase partitioning. Biotechnol. Lett. 23, 1625–1627.

Sharma A, Khare SK, Gupta MN. 2001b. Enzyme assisted aqueous extraction of rice bran oil. J. Am. Oil. Chem. Soc.78, 949–951.

Sharma A, Khare SK, Gupta MN. 2002. Three-phase partitioning for extraction of oil from soybean. Bioresour. Technol. 85, 327–329.

Sharma A, Gupta MN. 2004. Oil extraction from almond, apricot and rice bran by three-phase partitioning after ultrasonication. Eur. J. Lipid. Sci. Technol. 106, 183–186.

Sheema F, Nasirullah, Debnath S. 2016. Molecular finger printing of nutra-coconut oil with improved health protective phytoceuticals and its efficacy as frying medium. J. Am. Oil Chem. Soc. 93, 1301–1310.

Singh RK, Sarker BC, Kumbhar BK. 1999. Response surface analysis of enzyme assisted oil extraction factors for sesame, groundnut and sunflower seeds. J. Food Sci. Technol. 36, 511–514.

Sosulski K, Sosulski FW, Coxworth E. 1988. Carbohydrase hydrolysis of canola to enhance oil extraction with hexane. J. Am. Oil Chem. Soc. 65, 357–61.

Soto CG, Chamy R, Zuniga M. 2004. Effect of enzymatic application on Borage (Borago officinalis) oil extraction by cold pressing. J. Chem. Eng. Japan 37, 326–331.

Tan ZJ, Yang ZZ, Yi YJ, Wang HY, Zhou WL, Li FF, Wang CY. 2016. Extraction of oil from flaxseed (Linum usitatissimum L.) using enzyme-assisted three-phase partitioning. Appl. Biochem. Biotechnol. 179, 1325–35.

Tavanandi HA, Raghavarao KSMS. 2018. Recovery of chlorophylls from spent biomass of Arthrospira platensis obtained after extraction of phycobiliproteins. Bioresour. Technol. 271, 391–401.

Tzen JTC, Huang AHC. 1992. Surface structure and properties of plant seed oil bodies. J. Cell Biol. 117, 327–335.

Tzen JTC, Cao Y, Laurent P, Ratnayake C, Huang AHC. 1993. Lipids, proteins, and structure of seed oil bodies from diverse species. Plant Physiol. 101, 267–276.

Vidhate GS, Singhal RS. 2013. Extraction of cocoa butter alternative from kokum (Garcinia indica) kernel by three-phase partitioning. J. Food Eng. 117, 464–466.




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