Partición trifásica asistida por enzimas: una alternativa eficiente para la extracción de aceite de sésamo (Sesamum indicum L.).
DOI:
https://doi.org/10.3989/gya.1060182Palabras clave:
Aceite de sésamo, Hidrólisis de la pared celular, Método de extracción verde, Partición trifásica asistida por enzimas (EATPP), extracción por solvente (SE)Resumen
Se investigó la partición trifásica (TPP) para la extracción de aceite de semillas de Sesamum indicum L. Los parámetros del proceso, como la concentración de sal, la proporción de suspensión/t-butanol y el pH del sistema, fueron estandarizados. Las condiciones óptimas para la recuperación máxima de aceite utilizando TPP fueron una concentración de sulfato de amonio del 40% (p/v), una relación de suspensión/t-butanol de 1: 1 (v/v) y un pH del sistema de 5.0. Las semillas en polvo se sometieron a una partición trifásica asistida por enzimas (EATPP) que se trata previamente con pectinasa, proteasa y mezcla de ?-amilasa y amilo-glucosidasa (relación 1:1) seguida de TPP (como condiciones estandarizadas) y su eficacia como recuperación de aceite se compara con TPP y extracción con solventes (SE). De todas las enzimas estudiadas, la EATPP con pectinasa produjo la mayor recuperación de aceite (86.12%), que es mayor que la de TPP (78.24%). Los ácidos grasos libres, el índice de saponificación y de peróxidos fueron más bajos en el caso de TPP y EATPP en comparación con SE lo que indica mejor calidad del aceite.
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