Grasas y Aceites, Vol 71, No 3 (2020)

Enhancement of lipid productivity from a promising oleaginous fungus Aspergillus sp. strain EM2018 for biodiesel production: Optimization of culture conditions and identification


https://doi.org/10.3989/gya.0345191

E. M. Abdellah
Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt
orcid https://orcid.org/0000-0002-2446-5422

T. H. Ali
Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt
orcid https://orcid.org/0000-0002-3106-4467

D. A.M. Abdou
Microbiology Department, Faculty of Science, Ain Shams University, Egypt
orcid https://orcid.org/0000-0002-9627-721X

N. M. Hassanein
Microbiology Department, Faculty of Science, Ain Shams University, Egypt
orcid https://orcid.org/0000-0003-2086-4474

M. Fadel
Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt
orcid https://orcid.org/0000-0002-4271-3725

A. A. Karam El-Din
Microbiology Department, Faculty of Science, Ain Shams University, Egypt
orcid https://orcid.org/0000-0001-7165-2629

D. H. El-Ghonemy
Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt
orcid https://orcid.org/0000-0003-4233-5872

Abstract


Oleaginous fungi have recently gained increasing attention among different microorganisms due to their ability for lipid production for the preparation of biofuel. In the present study, a locally isolated fungus E45, identified genetically as Aspergillus sp. strain EM2018, was found to produce 25.2% of the total lipids content of its dry cell weight (DCW). Optimization of culture conditions was performed and lipid accumula­tion increased by about 2.4 fold (from 25.2% to 60.1% of DCW) when the fungus was grown for seven days in the potato dextrose (50 g/L) liquid medium at pH 5.0, incubation temperature at 30 ºC and inoculum size of 2 × 106 spore/mL. Supplementation of the medium with yeast extract and NaNO3 at a concentration of 0.05% as organic and inorganic nitrogen sources, respectively, increased lipid production (53.3% lipid/dry biomass). Gas chromatography analysis of fungal lipids revealed the presence of saturated (mainly palmitic acid C16:0 (33%) and lignoceric acid C24:0 (15%)) and unsaturated fatty acids in different proportions (mainly linoleic acid C18:2 (24.4%), oleica cid C18:1 (14%) and arachidonic C20:4 (7.4%). These findings suggest this new oleaginous fungus as a promising feedstock for various industrial applications and for the preparation of biodiesel.

Keywords


Aspergillus sp. strain EM2018; Biodiesel; GC analysis; Lipid production; Optimization

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