Protective effects of a wheat germ rich diet against the toxic influence of profenofos on rat tissue lipids and oxidative pentose phosphate shunt enzymes

Authors

  • G. A. Abdel-Rahim Central Lab. Pest. Agric. Res. Center, Ministry of Agric.
  • G. I. Mahmoud Biochem., Dept., Fac. Agric., Cairo Univ.

DOI:

https://doi.org/10.3989/gya.125810

Keywords:

Lipids profile, Profenofos, Rats, Wheat germ

Abstract


The effects of technical and formulated forms of profenofos on the metabolic lipid fractions of the liver, brain and kidneys as well as the activity of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD), which consider lipid related enzymes, were studied. The two forms of profenofos were given separately either orally or by dermal at doses of 1/20 LD50 for 3 months (one dose every 48 h). Total lipids and lipid fractions (cholesterol, triglycerides and phospholipid contents) decreased in the three studied organ tissues either in technical or formulated profenofos-induced rats compared with normal control animals. The highest effect was observed in the case of orally formulated profenofo induction, and the lowest was detected for the dermal technical one. The same trend was found in the activities of G6PD and 6PGD associated with lipid metabolism in the liver, brain and kidney tissues under the same conditions. On other hand, the treatment of profenofos-induced animals by feeding a wheat germ rich diet (as antioxidant agent) produced significant improvements in both lipid fraction content and enzyme activity. In addition, the effects of the wheat germ rich diet (α-tocopherol rich source) readjusted and improved the disturbed metabolic fractions of the lipid profiles in the profenofos-induced rats as well as their related enzyme activities (G6PD and 6PGD: oxidative pentose phosphate shunt).

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References

Abdel-Rahim EA and Abdel-Rahim GA. 2007. The hypointensive effects of carrot as a natural antioxidant on chlorpyrifos toxicity, nucleic acids and chromosomal aberration in albino rats. Egypt. J. Appl. Sci. 22, 427-444.

Abdel-Rahim EA and Abdel-Rahim GA. 2008. Biochemical effects of guava diet antioxidant as a hypointensive agent for dimethoate toxicity on energy and cytochrome- c respiratory system in the pesticide albino rats. J. Biol. Chem. Environ. Sci. 3, 111-126.

Abdel-Rahim EA, Abdel-Rahim GA, Fayed SA and Mahmoud GI. 2009. Antioxidant diet as protective agents against biochemical perturbation effects induced by cypermethrin on lipids and protein fractions as well as kidneys function of blood rat. Aust. J. Basic Appl. Sci. 3, 267-276.

Abdel-Rahim GA. 2009. Reduction of profenofos toxicity effects by α-tocopherol diet (wheat germ) on carbohydrate metabolism of male albino rats. Egypt. J. Nutr. 24, 1-24.

Abdel-Rahman GH, Farrag AR, El Sharkawy SL and Abdel-Aal WE. 2006. Effects of profenofos on antioxidant enzymes activities and gastric mucosa in rats. JASMR 1, 125-134.

Abdollahi M, Ranjbar A, Shadnia S, Nikfar S and Rezaiee A. 2004. Pesticides and oxidative stress a review. Med. Sci. Monit. 106, 141-147.

Abou-Zeid MM, El-Baroty G, Abdel-Rahim EA, Blankato J, Dary C, El-Sebae AH and Saleh MA. 1993. Malathion disposition in dermally and orally treated rats and impact on the blood serum acetylcholinesterase and protein profile. J. Environ. Sci. Health 828, 413-430.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. doi:10.1016/0003-2697(76)90527-3

Bus JS, Vinegar A and Brooks SM. 1978. Biochemical and physiological changes in lungs of rats exposed to a cadmium chloride aerosol. Am. Rev. Respir. Disease 118, 573-584. PMid:212968

Chaurchami AJ, Miller W and Adstein JDB. 1959. Determination of total and free cholesterol. Clin. Chem. 5 609-611. PMid:13812374

Coskum O, Kanter M, Korkmaz A and Oter S. 2005. Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. Pharmacol. Res. 51, 117-123. PMid:15629256

COT. 1999. Organophosphates (Committee on toxicity of chemicals in food, consumer products and the Environmental) London, UK, Department of Health.

Dwivedi PD, Mukul D and Khanna SK. 1998. Role of cytochrome P-450 in quinalphos toxicity: Effect on hepatic and brain antioxidant enzymes in rats. Food Chem. Toxicol. 36, 437-444. doi:10.1016/S0278-6915(97)00165-8

Fisher WT, Edwards NM and Lehman RW. 1964. Simplified assay of vitamins A and E in mixtures. J. Pharm. Sci. 53, 294-298. doi:10.1002/jps.2600530310 PMid:14185016

Frei B, England L and Ames BN. 1989. Ascorbate is an outstanding antioxidant in human blood plasma. Proc. Natl. Acad. Sci. USA 86, 6377-6381. doi:10.1073/pnas.86.16.6377

Glock GE and McLean P. 1953. Further studies on the properties and assay of glucose-6-phosphate dehydrogenase and 6-phosphogylconate dehydrogenase of rat liver. Biochem. J. 55, 400-408. PMid:13105646    PMCid:1269290

Goel A, Dani V and Dhawan DK. 2005. Protective effects of zinc on lipid peroxidation antioxidant enzyme and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chem. Biol. Interact. 156, 131-140. doi:10.1016/j.cbi.2005.08.004 PMid:16169541

Goel A, Dani V and Dhawan DK. 2007. Zinc mediates normalization of hepatic drug metabolizing enzymes in chlorpyrifos-induced toxicity. Toxicol. Letters 169, 26-33. doi:10.1016/j.toxlet.2006.07.342 PMid:17194553

Grover P, Danadevi K, Mahboob M, Rozati B, Saleha B and Rahman MF. 2003. Evaluation of genetic damage in workers employed in pesticide production utilizing the comet assay. Mutagenesis 18, 201-205. doi:10.1093/mutage/18.2.201 PMid:12621077

Hayes JD, Flanagan JU and Jowsey IR. 2005. Glutathione transferases. Annu. Rev. Pharmacol. Toxicol. 45, 51-88. doi:10.1146/annurev.pharmtox.45.120403.095857 PMid:15822171

Ibrahim AM, Zaid MA, El-Maholy LD and Abou-Zeid MM. 1990. Studies on crude wheat germ produced as a by-product of milling industry. Ann. Agric. Sci. Moshtohor 28, 1189-1200.

Jacob RA and Burri BJ. 1996. Oxidative damage and defense. Am. J. Clin. Nutr. 63, 985S-990S. PMid:8644698

Joseph RK, Shauma A and Ames M. 1972. Determination of total lipid in blood serum. Powl. Clin. Chem. 18, 199-201.

Kamath V, Joshi AKR and Rajini PS. 2008. Dimethoate induced biochemical perturbations in rat pancreas and its attenuation by cashew nut skin extract. Pesticide Biochem. Physiol. 90, 58-65. doi:10.1016/j.pestbp.2007.07.007

Karaoz E, Gultekin F, Akdogan M, Oncu M and Gokciman A. 2002. Protective role of melatonin and a combination of vitamin C and vitamin E on lung toxicity induced by chlorpyrifos-ethyl in rats. Exp. Toxic. Pathol. 54, 97-108. doi:10.1078/0940-2993-00236 PMid:12211644

Kaur S and Dhanju CK. 2005. Biochemical effects of some organophosphorus pesticides on the ovaries of albino rats. Indian J. Physiol. Pharmacol. 49, 148-152. PMid:16170982

Ketes M. 1972. Techniques of lipidology. Isolation, analysis and identification of lipids. Amsterdam: North Holland and Publishing Co.

Klaunig JE, Xu Y, Isenberg JS, Bachowski S, Kolaja KL, Jiang J, Stevenson DE and Walborg EF. 1998. The role of oxidative stress in chemical carcinogenesis. Environ. Health Prospect. 106 289-295. PMid:9539021    PMCid:1533298

Kruger NJ, von Schaewen A. 2003. The oxidative pentose phosphate pathway: structure and organisation. Curr. Opin. Plant Biol. 6, 236-46. doi:10.1016/S1369-5266(03)00039-6

Lane-Peter WA and Pearson AE. 1971. Dietary requirements In: The Laboratory Animal Principles and Practice. pp. 142. Academic press. London and New York.

Leenhardt F, Fardet A, Lyan B, Gueux E, Rock E, Mazur A, Chanliaud E, Demign[ C and R[m[sy C. 2008. Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues. J. Am. Coll. Nutr. 27, 222-228. PMid:18689553

Lukaszewicz-Hussain A. 2001. Organophosphate insecticide chlorfenvinphos affects superoxide dismutase, catalase and malondialdehyde in rat liver. Polish J. Environ. Studies 10, 279-282.

Lukaszewicz-Hussain A and Moniuszko-Jakoniuk J. 2003. Organphosphate insecticide chlorfenvinphos affects enzymatic and non-enzymatic antioxidants in erythrocytes and serum of rats. Polish J. Environ. Studies 12, 417-423.

Mahboob M, Kaleem M and Siddiqui J. 2004. Effects of a noval organophosphorus pesticide (RPR-V) on extra hepatic detoxifying enzymes after repeated oral doses in rats. Toxicol. 202, 159-164. doi:10.1016/j.tox.2004.05.004 PMid:15337579

Mehta A, Mason PJ, Vulliamy TJ. 2000. Glucose-6- phosphate dehydrogenase deficiency. Baillieres Best Pract. Res. Clin. Haematol. 13, 21-38. doi:10.1053/beha.1999.0055

Middle Brooks J. 1977. Statistical calculation. How to solve statistical problem. Annals Arboreum Science 62, 21-27.

Murray RK, Grennerm DK, Mayes PA and Rodwell VW. 2006. Harper’s illustrated biochemistry (27th ed) Mc-Graw Hill Comp. Inc. Boston, New York, Singapora.

Noguchi C and Nikki E. 2000. Phenolic antioxidants: a rationale for design and evaluation of novel antioxidant drug for atherosclerosis. Free Rad. Biol. Med. 28, 1538-1546. doi:10.1016/S0891-5849(00)00256-2

Ohkatsu Y, Kajiyama T and Arai Y. 2001. Antioxidant activities of tocopherols. Polym. Degrad. Stabil. 72, 303-311. doi:10.1016/S0141-3910(01)00022-2

Oral B, Guney M, Demirin H, Ozguner M, Giray SG, Take G, Mungan T and Altuntas I. 2006. Endometrial damage and apoptosis in rats induced by dichlorvos and ameliorating effect of antioxidant vitamin E and C. Reprod. Toxicol. 22, 783-790. doi:10.1016/j.reprotox.2006.08.003 PMid:16973328

Parke DV and Piotrowski JK. 1996. Glutathione: its role in detoxication of reactive oxygen species and environmental chemicals. Acta Pol. Toxicol. 4, 1-14.

Pennington JA. 1989. Food values of portions commonly used. (15th ed). Perennial Library, Harper and Row Publishers. Inc. New York, pp. 104 and 282.

Piras A, Rosa A, Falconieri D, Porcedda S, Dessì MA and Marongiu B. 2009. Extraction of oil from wheat germ by supercritical CO2. Molecules 14, 2573-2581. doi:10.3390/molecules14072573 PMid:19633624

Ray DE and Richards PG. 2001. The potential for toxic effects of chronic, low-dose exposure to organophosphates. Toxicol. Lett. 12, 343-351. doi:10.1016/S0378-4274(01)00266-1

Rohrer A, Hiaber TE, Melnyk LJ and Berry MR. 2003. Transfer efficiencies of pesticides from household flooring surfaces to food. J. Expo. Anal. Environ. Epidemiol. 13, 454-464. doi:10.1038/sj.jea.7500300 PMid:14603346

Salvemini F, Franze A, Iervolino A, Filosa S, Salzano S and Ursini MV. 1999. Enhanced glutathione levels and oxidoresistance mediated by increased glucose- 6-phosphate dehydrogenase expression. J. Biol. Chem. 274, 2750-2757. doi:10.1074/jbc.274.5.2750 PMid:9915806

Stephen B, Kyle L, Yong X, Cynthia A, Donald L, Karl I and James L. 1997. Role of oxidative stress in the mechanism of dieldrin’s hepatotoxicity. Ann. Clin. Lab. Sci. 27, 196-208. PMid:9142372

Tomlin CDS. 2000. Pesticide Manual, A world compendium the pesticide manual (20th ed). British crop protection Council. pp. 230-231.

USEPA. 2000. Health and environmental affects profile for Dichlorvos U.S. Environmental Protection Agency. 11-15.

Verma RS, Mehta A and Srivastava N. 2007. In vivo chlorpyrifos induced ox idative stress: Attenuation by antioxidant vitamins. Pest. Biochem. Physiol. 88, 191-196. doi:10.1016/j.pestbp.2006.11.002

Young A and Pestaner DL. 1975. Determination of triglyceride in serum. Clin. Chem. 21, 5-7. PMid:234815

Zhang Z, Apse K, Pang J and Stanton RC. 2000. High glucose inhibits glucose-6-Phosphate dehydrogenase via cAMP in aortic endothelial cells. J. Biol. Chem. 275, 40042-40047. doi:10.1074/jbc.M007505200 PMid:11007790

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Published

2011-09-30

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Abdel-Rahim GA, Mahmoud GI. Protective effects of a wheat germ rich diet against the toxic influence of profenofos on rat tissue lipids and oxidative pentose phosphate shunt enzymes. grasasaceites [Internet]. 2011Sep.30 [cited 2021Dec.9];62(3):344-52. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1333

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