Statistical model for classifying the feeding systems of Iberian pigs through Gas Chromatography (GC-FID) and Isotope Ratio Mass Spectrometry (GC-C-IRMS)
DOI:
https://doi.org/10.3989/gya.130412Keywords:
Fatty acids, Gas Chromatography, GCC- IRMS, Iberian pig, Isotope Ratio Mass Spectrometry, Subcutaneous fatAbstract
In the present work we have analyzed a total of 734 subcutaneous fat samples from Iberian pigs with different feeding systems for fattening (“Bellota”, “Recebo”, “Campo” and “Cebo”) over three consecutive years, 2009-2011. Lipids were extracted from the subcutaneous fat on the rump, and after esterification, they were analyzed by Gas Chromatography (GC-FID) and Gas Chromatography- Combustion-Isotope Ratio Mass Spectrometry (GC-C-IRMS). Mean fatty acids and isotope ratios show that there are differences according to the year and feeding systems, two factors that should be taken into account when classifying the animals. The application of different prediction models based on Discriminant analysis has allowed us to establish a method for the classification of animals according to the feeding system type, with a correct percentage of 85% using three or four classification categories (Bellota, Recebo, Campo and/or Cebo) and 91% using only two categories, Cebo and Bellota. This model could provide the basis for appropriate classification of Iberian pigs according to their feeding regime.
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Boletín Oficial del Estado (B.O.E.) 2004. ORDEN PRE/3844/2004, de 18 de noviembre, por la que se establecen los métodos oficiales de toma de muestras en canales de cerdos ibéricos y el método de análisis para la determinación de la composición de ácidos grasos de los lípidos totales del tejido adiposo subcutáneo de cerdos ibéricos. 38770-38779. Madrid, Spain.
Boletín Oficial del Estado (B.O.E.) 2007a. REAL DECRETO 1469/2007, de 2 de noviembre, por el que se aprueba la norma de calidad para la carne, el jamón, la paleta y la caña de lomo ibéricos. 45087-45104. Madrid, Spain.
Boletín Oficial del Estado (B.O.E.) 2007b. ORDEN APA/3653/2007, de 13 de diciembre, por la que se publican los valores de ácidos grasos aplicables a las designaciones de alimentación «Bellota» y «Recebo», para la campa-a 2007-2008. 51655. Madrid, Spain.
Boletín Oficial del Estado (B.O.E.) 2010. Orden ARM/2139/2010, de 16 de julio, por la que se extiende el Acuerdo de la Asociación Interprofesional del Cerdo Ibérico, al conjunto del sector, y se fija la aportación económica obligatoria, para realizar actividades de promoción de los productos del cerdo ibérico, mejorar la información y conocimiento sobre las producciones y los mercados, y realizar programas de investigación, desarrollo, innovación tecnológica y estudios, para las campa-as 2010-2011, 2011-2012 y 2012-2013.68100-68102. Madrid, Spain.
Cava R, Andrés A. 2001. La obtención de material prima de una adecuada aptitud tecnológica. Características de la grasa determinantes de la calidad del jamón: influencia de los factores genéticos y ambientales, en Ventanas J (Ed). Tecnología del Jamón Ibérico. Ed. Mundi-Prensa, Madrid, Spain, 99-129.
De Niro MJ, Epstein S. 1978. Influence of diet on the distribution of carbon isotope ratios in animals. Geochim. Cosmochim. Acta 42, 495-506. http://dx.doi.org/10.1016/0016-7037(78)90199-0
García-Casco JM, Mu-oz M, González E. 2013. Predictive ability of the feeding system by means of several analytical methods in iberian pig. Grasas Aceites 64, 191-200. http://dx.doi.org/10.3989/gya.130812
García-Olmo J, Garrido-Varo A, De Pedro E. 2009. Classification of real farm conditions Iberian pigs according to the feeding regime with multivariate models developed by using fatty acids composition or NIR spectral data. Grasas Aceites 60, 233-237. http://dx.doi.org/10.3989/gya.130408
Gilles E. 2009. Dry cured ham quality as related to lipid quality of raw material and lipid changes during processing: a review. Grasas Aceites 60, 297-307. http://dx.doi.org/10.3989/gya.130908
Goodman KJ, Brenna JT. 1992. High sensitivity tracer detection using high-precision gas chromatographycombustion isotope ratio mass spectrometry and highly enriched [U–13C] precursors. Anal. Chem. 64, 1088-1095. http://dx.doi.org/10.1021/ac00034a004 PMid:1609955
González Martín I, González Pérez C, Hernández Méndez J, Recio Hernández C, Sabio Rey E. 1998. Método para la caracterización y diferenciación del cerdo ibérico en función de la dieta. ES 2 100 130 B1.
González-Martín I, González-Pérez C, Hernández- Méndez J, Marqués-Macias E, Sanz-Poveda F. 1999. Use of isotope analysis to characterize meat from iberian Breed Swine. Meat Sci. 52, 437-441. http://dx.doi.org/10.1016/S0309-1740(99)00027-3
González-Martín I, González-Pérez C, Hernández-Méndez J, Sánchez-González C. 2001. Differentiation of dietary regimen of iberian swine by means of isotopic analysis of carbon and sulpur in hepatic tissue. Meat Sci. 58, 25-30. http://dx.doi.org/10.1016/S0309-1740(00)00126-1
Kelly SD, Parker I, Sharman M, Dennis MJ. 1997. Assessing the authenticity of single seed vegetable oils using fatty acid stable carbon isotope ratios (13C/12C). Food Chem. 59, 181-186. http://dx.doi.org/10.1016/S0308-8146(96)00286-5
Kelly SD, Rhodes C. 2002. Emerging Techniques in Vegetable Oil Analysis Using Stable Isotope Ratio Mass Spectrometry Grasas Aceites 53, 34-44.
Melgar J, Cid C, Astiasarán I, Bello J. 1991. Influencia de la alimentación del cerdo ibérico en las características de los compuestos relacionados con la grasa del jamón curado. Grasas Aceites. 42, 51-55. http://dx.doi.org/10.3989/gya.1991.v42.i1.1278
Narváez-Rivas M, León-Camacho M, Vicario IM. 2009. Fatty acid and triacylglycerol composition of the subcutaneous fat from iberian pigs fattened on the traditional fee: "Montanera". Effect of anatomical location and length of feeding. Grasas Aceites 60, 238-247. http://dx.doi.org/10.3989/gya.130308
Recio Hernández C. 2010. Método de identificación de productos alimenticios. ES2 326 249 B1. REGLAMENTO (CEE) No 2676/90 DE LA COMISIÓN de 17 de septiembre de 1990 por el que se determinan los métodos de análisis comunitarios aplicables en el sector del vino.
Ruiz J, Pretón MJ. 2001. Métodos para la clasificación de la materia prima, en Ventanas J (Ed). Tecnología del Jamón Ibérico. Ed. Mundi-Prensa, Madrid, Spain, 130-160.
Ruiz J, Ventanas J, Cava R, Andrés AI, García C. 2000. Texture and appearance of dry cured ham as affected by fat content and fatty acid composition. Food Research Int. 33, 91-95. http://dx.doi.org/10.1016/S0963-9969(99)00153-2
Spangenberg J E, Macko SA, Hunziker J. 1998. Characterization of olive oil by carbon isotope analysis of individual fatty acids: Implications for authentication. J. Agric. Food Chem. 46, 4179-4184. http://dx.doi.org/10.1021/jf980183x
Statgraphics Centurion XVI. 16.1.15 version. StatPoint Technologies, Inc. 1982-2011.
Ventanas J, Andrés AI, Cava R, Tejeda JF, y Ruiz J. 1999. Composición y características de la grasa en el cerdo Ibérico e influencia sobre la calidad del jamón. Cárnica 2000 Marzo, 55-59.
Ventanas S, Estévez M, Tejeda JF, Ruiz J. 2006. Protein and lipid oxidation in dry cured loin as affected by crossbreeding and diet. Meat Sci. 72, 647-655. http://dx.doi.org/10.1016/j.meatsci.2005.09.011 PMid:22061875
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