Characterization of different lipolytic fractions in Carica papaya
Keywords:Carica papaya, hidrólisis, látex, lipasa, triglicéridos
Carica papaya latex contains interesting enzymes; the best known is papain, but lipolytic activity is also present. Due to the complexity of the latex polymeric matrix, it has not been possible to completely isolate enzymes responsible of lipolytic activity. The aim of this work was to characterize the lipolytic activity in the raw latex (CPLtx) and two partially purified fractions of papaya latex (without protease, CPL-p and without esterase CPL-e). Thermostability, optimal temperature and pH in the hydrolysis of two model triglycerides (tributyrin and triolein) and the selectivity towards triglycerides with different chain lengths were determined. The lipolytic activity of these biocatalysts in the hydrolysis of tributyrin and olive oil was similar to other commercially available immobilized microbial lipases (RM IM and Novozyme 435).
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