Characterization of different lipolytic fractions in Carica papaya


  • I. Rivera Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ)
  • G. Sandoval Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ)



Carica papaya, hidrólisis, látex, lipasa, triglicéridos


Carica papaya latex contains interesting enzymes; the best known is papain, but lipolytic activity is also present. Due to the complexity of the latex polymeric matrix, it has not been possible to completely isolate enzymes responsible of lipolytic activity. The aim of this work was to characterize the lipolytic activity in the raw latex (CPLtx) and two partially purified fractions of papaya latex (without protease, CPL-p and without esterase CPL-e). Thermostability, optimal temperature and pH in the hydrolysis of two model triglycerides (tributyrin and triolein) and the selectivity towards triglycerides with different chain lengths were determined. The lipolytic activity of these biocatalysts in the hydrolysis of tributyrin and olive oil was similar to other commercially available immobilized microbial lipases (RM IM and Novozyme 435).


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Abdelkafi S, Barouh N, Fouquet B, Fendri I, Pina M, Scheirlinckx F, Villeneuve P, Carriere F. 2011. Carica papaya Lipase: A Naturally Immobilized Enzyme with Interesting Biochemical Properties. Plant Food Human Nutr. 66, 34–40.

Abdelkafi S, Ogata H, Barouh N, Fouquet B, Lebrun R, Pina M, Scheirlinckx F, Villeneuve P, Carriere F. 2009. Identification and biochemical characterization of a GDSL-motif carboxylester hydrolase from Carica papaya latex. BBA-Mol Cell Biol Lipids. 1791, 1048–1056.

Bhardwaj K, Raju A, Rajasekharan R. 2001. Identification, purification, and characterization of a thermally stable lipase from rice bran. A new member of the (Phospho) lipase family. Plant Physiol. 127, 1728–1738.

Bordes F, Tarquis L, Nicaud JM, Marty A. 2011. Isolation of a thermostable variant of Lip2 lipase from Yarrowia lipolytica by directed evolution and deeper insight into the denaturation mechanisms involved. J Biotechol. 156, 117–124.

Cambon E, Rodriguez JA, Pina M, Arondel V, Carriere F, Turon F, Ruales J, Villeneuve P. 2008. Characterization of typo-, regio-, and stereo-selectivities of babaco latex lipase in aqueous and organic media. Biotechnol Lett. 30, 769–774.

Casas-Godoy L, Duquesne S, Bordes F, Sandoval G, Marty A. 2012. Lipases: an overview. in Sandoval G (Ed). Methods in molecular biology, Lipases and phospholipases. 547. Springerlink. pp. 3–30.

Chen CC, Tsai SW, Villeneuve P. 2005. Enantioselective hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents via lipases from Carica pentagona Heilborn and Carica papaya. J Mol Catal B-Enz. 34, 51–57.

Dhouib R, Laroche-Traineau J, Shaha R, Lapaillerie D, Solier E, Ruales J, Pina M, Villeneuve P, Carriere F, Bonneu M, Arondel V. 2011. Identification of a putative triacylglycerol lipase from papaya latex by functional proteomics. Febs J. 278, 97–110.

Dhuique-Mayer C, Caro Y, Pina M, Ruales J, Dornier M, Graille J. 2001. Biocatalytic properties of lipase in crude latex from babaco fruit (Carica pentagona). Biotechnol Lett. 23, 1021–1024.

Ejedegba BO, Onyeneke EC, Oviasogie PO. 2007. Characteristics of lipase isolated from coconut (Cocos nucifera linn) seed under different nutrient treatments. Afr. J. Biotechnol. 6, 723–727.

El Moussaoui A, Nijs M, Paul C, Wintjens R, Vincentelli J, Azarkan M, Looze Y. 2001. Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defense mechanism. Cell Mol. Life Sci. 58, 556–570.

Giordani R, Moulin A, Verger R. 1991. Tributyroylglycerol hydrolase activity in Carica-papaya and other lattices. Phytochemistry. 30, 1069–1072.

Hasan F, Shah AA, Javed S, Hameed A. 2010. Enzymes used in detergents: Lipases. Afr. J. Biotechnol. 9, 4836–4844.

Lee KT, Foglia TA. 2000. Synthesis, purification, and characterization of structured lipids produced from chicken fat. J. Am. Oil Chem. Soc. 77, 1027–1034.

Ng IS, Tsai SW. 2005. Partially purified Carica papaya lipase: a versatile biocatalyst for the hydrolytic resolution of (R,S)-2-arylpropionic thioesters in water-saturated organic solvents. Biotechnol. Bioeng. 91, 106–113.

Wei-Ning Niu, Zhao-Peng Li, Da-Wei Zhang, Ming-Rui Yu, Tian-Wei Tan Niu. 2006. Improved thermostability and the optimum temperature of Rhizopus arrhizus lipase by directed evolution. J. Mol. Catal. B-Enz. 43, 33–39.

Nieto S, Gutiérrez J, Sanhueza, Valenzuela A. 1999. Preparation of sn-2 long-chain polyunsaturated monoacylglycerols from fish oil by hydrolysis with a stereo-specific lipase from Mucor miehei. Grasas Aceites 50, 111–113.

Noma A, Borgstro B. 1971. Acid lipase of castor beans - positional specificity and reaction mechanism. Biochim. Biophys. Acta. 227, 106–110.

Pinyaphong P, Phutrakul S. 2009. Synthesis of Cocoa Butter Equivalent from Palm Oil by Carica papaya Lipase-Catalyzed Interesterification. Chiang Mai J. Sci. 36, 359–368.

Quintana PG, Sandoval G, Baldessari A. 2011. Lipase-catalyzed synthesis of medium- and long-chain diesters of 2-oxoglutaric acid. Biocatal. Biotransf. 29, 186–191.

Rivera I, Mateos-Diaz JC, Sandoval G. 2012. Plant Lipases: Partial Purification of Carica papaya Lipase. in Sandoval G (ed). Methods in molecular biology, Lipases and phospholipases. pp 547. 1940–6029. Springerlink. pp. 115–122.

Rivera I, Mateos JC, Marty A, Sandoval G, Duquesne S. 2013. Lipase from Carica papaya latex presents high enantioselectivity towards the resolution of prodrug (R, S)-2-bromophenylacetic acid octyl ester. Tetrahedron Lett. In press.

Silva LG, Garcia O, Lopes MTP, Salas CE. 1997. Changes in protein profile during coagulation of latex from Carica papaya. Braz. J. Med. Biol. Res. 30, 615–619.

Singh AK, Mukhopadhyay M. 2012. Olive oil glycerolysis with the immobilized lipase Candida antarctica in a solvent free system. Grasas Aceites 63, 202–208.

Staubmann R, Ncube I, Gubitz GM, Steiner W, Read JS. 1999. Esterase and lipase activity in Jatropha curcas L-seeds. J. Biotechnol. 75, 117–126.

Tecelao C, Rivera I, Sandoval G, Ferreira-Dias S. 2012. Carica papaya latex: A low-cost biocatalyst for human milk fat substitutes production. Eur. J. Lipid Sci. Tech. 114, 266–276.

Tuter M. 1998. Castor bean lipase as a biocatalyst in the esterification of fatty acids to glycerol. J. Am. Oil Chem. Soc. 75, 417–420.

Wang YJ, Sheu JY, Wang FF, Shaw JF. 2012. Lipase-Catalyzed Oil Hydrolysis in the Absence of Added Emulsifier. Biotechnol. Bioeng. 31, 628–633.

Zheng Y-Y, Guo X-H, Song N-N, Li DC. 2011. Thermophilic lipase from Thermomyces lanuginosus: Gene cloning, expression and characterization. J. Mol. Catal. B: Enz. 69, 127–132.



How to Cite

Rivera I, Sandoval G. Characterization of different lipolytic fractions in Carica papaya. grasasaceites [Internet]. 2014Mar.30 [cited 2022Nov.29];65(1):e003. Available from: