Enhancement of lipid productivity from a promising oleaginous fungus <em>Aspergillus</em> sp. strain EM2018 for biodiesel production: Optimization of culture conditions and identification

E. M. Abdellah; T. H. Ali; D. A.M. Abdou; N. M. Hassanein; M. Fadel; A. A. Karam El-Din; D. H. El-Ghonemy

doi:10.3989/gya.0345191

Authors

E. M. Abdellah Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre https://orcid.org/0000-0002-2446-5422
T. H. Ali Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre https://orcid.org/0000-0002-3106-4467
D. A.M. Abdou Microbiology Department, Faculty of Science, Ain Shams University https://orcid.org/0000-0002-9627-721X
N. M. Hassanein Microbiology Department, Faculty of Science, Ain Shams University https://orcid.org/0000-0003-2086-4474
M. Fadel Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre https://orcid.org/0000-0002-4271-3725
A. A. Karam El-Din Microbiology Department, Faculty of Science, Ain Shams University https://orcid.org/0000-0001-7165-2629
D. H. El-Ghonemy Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre https://orcid.org/0000-0003-4233-5872

DOI:

https://doi.org/10.3989/gya.0345191

Keywords:

Aspergillus sp. strain EM2018, Biodiesel, GC analysis, Lipid production, Optimization

Abstract

Oleaginous fungi have recently gained increasing attention among different microorganisms due to their ability for lipid production for the preparation of biofuel. In the present study, a locally isolated fungus E45, identified genetically as Aspergillus sp. strain EM2018, was found to produce 25.2% of the total lipids content of its dry cell weight (DCW). Optimization of culture conditions was performed and lipid accumulation increased by about 2.4 fold (from 25.2% to 60.1% of DCW) when the fungus was grown for seven days in the potato dextrose (50 g/L) liquid medium at pH 5.0, incubation temperature at 30 ºC and inoculum size of 2 × 10⁶ spore/mL. Supplementation of the medium with yeast extract and NaNO₃ at a concentration of 0.05% as organic and inorganic nitrogen sources, respectively, increased lipid production (53.3% lipid/dry biomass). Gas chromatography analysis of fungal lipids revealed the presence of saturated (mainly palmitic acid C16:0 (33%) and lignoceric acid C24:0 (15%)) and unsaturated fatty acids in different proportions (mainly linoleic acid C18:2 (24.4%), oleica cid C18:1 (14%) and arachidonic C20:4 (7.4%). These findings suggest this new oleaginous fungus as a promising feedstock for various industrial applications and for the preparation of biodiesel.

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