Chemical compositon, antibacterial and antioxidant activities of Cnidium silaifolium ssp. orientale (Boiss.) Tutin essential oils




Antimicrobial, Antioxidant, Apiaceae, Cnidium silaifolium ssp. orientale


The chemical compositions of the essential oils (EOs) obtained by hydrodistillation from different parts of Cnidium silaifolium ssp. orientale (Boiss.) Tutin were analyzed both by GC-FID and GC/MS, simultaneously. One hundred nine compounds representing 90.1% of the total volatiles in the EOs were identified with the main characteristic compounds α-pinene (50.3%) in the root, germacrene D (20.3%) in the fruit, and β-caryophyllene (18.7%) in the aerial parts of C. silaifolium ssp. orientale. The antimicrobial activity against human pathogenic Gram-negative and Gram-positive bacteria was evaluated by the in vitro microdilution method. Antibacterial succeptibility was observed from the root and aerial part EOs against Staphylococcus aureus (0.039 and 0.156 mg/mL, respectively); while the fruit EO was most effective against Bacillus cereus at 0.07 mg/mL. The antioxidant capacities of the EOs were also evaluated by in vitro DPPH• and ABTS•+ scavenging assays, where no significant activity was observed compared to ascorbic acid and Trolox.


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Aligiannis N, Kalpoutzakis E, Kyriakopoulou I, Mitaku S, Chinou IB. 2004. Essential oils of Phlomis species growing in Greece: Chemical composition and antimicrobial activity. Flavour Fragr. J. 19, 320–324.

Arici M, Sagdic O, Gecgel U. 2005. Antibacterial effect of Turkish black cumin (Nigella sativa L.) oils. Grasas Aceites 56, 259–262.

Başer KHC, Buchbauer G. 2016. Handbook of Essential Oils Science, Technology and Applications. 2nd edition. London, CRC Press, 121-130.

Demirci F, Karaca N, Tekin M, Demirci B. 2018. Anti-inflammatory and antibacterial evaluation of Thymus sipyleus Boiss. subsp. sipyleus var. sipyleus essential oil against rhinosinusitis pathogens. Microb. Pathog. 122, 117–121.

Hong H, An JC, De La Cruz JF, Hwang SG. 2017. Cnidium officinale makino extract induces apoptosis through activation of caspase-3 and p53 in human liver cancer HepG2 cells. Exp. Ther. Med. 14, 3191–3197.

Jeong JB, Ju SY, Park JH, Lee JR, Yun KW, Kwon ST, Lim JH, Chung GY, Jeong HJ. 2009. Antioxidant activity in essential oils of Cnidium officinale makino and Ligusticum chuanxiong hort and their inhibitory effects on DNA damage and apoptosis induced by ultraviolet B in mammalian cell. Cancer Epidemiol. 33, 41–46.

Kapetanos C, Karioti A, Bojović S, Marin P, Veljić M, Skaltsa H. 2008. Chemical and principal-component analyses of the essential oils of Apioideae taxa (Apiaceae) from Central Balkan. Chem Biodivers. 5, 101–119.

Karadağ AE, Demirci B, Çaşkurlu A, Demirci F, Okur ME, Orak D, Sipahi H, Başer KHC. 2019. In vitro antibacterial, antioxidant, anti-inflammatory and analgesic evaluation of Rosmarinus officinalis L. flower extract fractions. South African J. Bot. 125, 214–220.

Karadag AE, Demirci B, Cecen O, Tosun F. 2019. Chemical characterization of Glaucosciadium cordifolium (Boiss.) B.L. Burtt and P.H. Davis essential oils and their antimicrobial, and antioxidant activities. Istanbul J. Pharm. 49, 77–80.

Kim K-T, Kim M-H, Park J-H, Lee J-Y, Cho H-J, Yoon I-S, Kim D-D. 2018. Microemulsion-based hydrogels for enhancing epidermal/dermal deposition of topically administered 20(S)-protopanaxadiol: in vitro and in vivo evaluation studies. J. Ginseng Res. 42, 512–523.

Li YM, Jia M, Li HQ, Zhang ND, Wen X, Rahman K, Zhang QY, Qin LP. 2015. Cnidium monnieri: A review of traditional uses, phytochemical and ethnopharmacological properties. Am. J. Chin. Med. 43, 835–877.

Lim EG, Kim GT, Kim BM, Kim EJ, Kim SY, Kim YM. 2018. Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53-independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells. Mol. Med. Rep. 17, 2572–2580.

Oh H, Kim JS, Song EK, Cho H, Kim DH, Park SE, Lee HS, Kim YC. 2002. Sesquiterpenes with hepatoprotective activity from Cnidium monnieri on tacrine-induced cytotoxicity in Hep G2 cells. Planta Med. 68, 748–749.

Polat T, Özer H, Cakir A, Kandemir A, Mete E, Özturk E, Yildiz G. 2011. Volatile Constituents of Cnidium silaifolium (Jacq.) Simonkai subsp. orientale (Boiss.) Tutin from Turkey. J. Essent. Oil-Bearing Plants 14, 453–457.

Selim SA. 2011. Chemical composition, antioxidant and antimicrobial activity of the essential oil and methanol extract of the egyptian lemongrass cymbopogon proximus stapf. Grasas Aceites 62, 55–61.

Sim Y, Shin S. 2014. Antibacterial activities of the essential oil from the leaves and rhizomes of Cnidium officinale Makino. J. Essent. Oil Res. 26, 452–457.

Tatsadjieu LN, Essia Ngang JJ, Ngassoum MB, Etoa FX. 2003. Antibacterial and antifungal activity of Xylopia aethiopica, Monodora myristica, Zanthoxylum xanthoxyloïdes and Zanthoxylum leprieurii from Cameroon. Fitoterapia 74, 469–472.

Tran HNK, Cao TQ, Kim JA, Youn UJ, Kim S, Woo MH, Min BS. 2018. Anti-inflammatory activity of compounds from the rhizome of Cnidium officinale. Arch. Pharm. Res. 41, 977–985.

Yüzbaşıoğlu S, Altınözlü H, Kandemir A, Özbek MU. 2018. Flora of Kemaliye (Erzincan) District. Hacettepe J. Biol. Chem. 4, 533–557.



How to Cite

Karadağ A, Demirci B, Çeçen Ö., Tosun F, Demirci F. Chemical compositon, antibacterial and antioxidant activities of Cnidium silaifolium ssp. orientale (Boiss.) Tutin essential oils. grasasaceites [Internet]. 2021Jun.3 [cited 2021Jul.28];72(2):e403. Available from: