Color of extra virgin olive oils enriched with carotenoids from microalgae: influence of ultraviolet exposure and heating

M.C.  Murillo; A.B.  García; T.  Lafarga; M.  Melgosa; R.  Bermejo

doi:10.3989/gya.0104211

Authors

M.C. Murillo Department of Physical and Analytical Chemistry, Linares High Polytechnic School (EPSL), University of Jaén https://orcid.org/0000-0003-4927-4312
A.B. García Department of Physical and Analytical Chemistry, Linares High Polytechnic School (EPSL), University of Jaén https://orcid.org/0000-0001-7496-4628
T. Lafarga Department of Chemical Engineering, Almería University https://orcid.org/0000-0002-1923-7214
M. Melgosa Department of Optics, University of Granada https://orcid.org/0000-0002-7226-4190
R. Bermejo Department of Physical and Analytical Chemistry, Linares High Polytechnic School (EPSL), University of Jaén https://orcid.org/0000-0002-8960-3232

DOI:

https://doi.org/10.3989/gya.0104211

Keywords:

Extra virgin olive oil, Scenedesmus almeriensis, CIELAB system, Carotenoids

Abstract

A carotenoid-rich extract containing 2.5 mg/mL of lutein and 3.3 mg/mL of β-carotene from the microalga Scenedesmus almeriensis was added to ten extra virgin olive oils from four Spanish cultivars with differing degrees of ripeness, obtaining carotenoid enriched oils with lutein and β-carotene concentrations of 0.082 and 0.11 mg/mL, respectively. Extra virgin olive oils enriched with carotenoids from microalgae were studied by analyzing the effect on color of three different treatments: ultraviolet exposure, microwave heating and immersion bath heating. The methodology was designed to simulate, in controlled laboratory conditions, the effects of household treatments. Spectrophotometric color measurements were then performed to monitor color changes in the enriched and non-enriched extra virgin olive oil samples. Enriched oils are much more chromatic, darker and redder than natural oils. After 55 days UV irradiation, 40 min microwave heating, and 72 hours thermostatic heating, the average color differences for natural/enriched extra virgin olive oils were 98/117, 15/9 and 57/28 CIELAB units, respectively. In general, increasing temperature and ultraviolet exposure produced higher CIELAB color differences in the non-enriched samples. The addition of microalga extracts to extra virgin olive oils was found to induce some color stability and may constitute a future way of increasing the daily intake of beneficial bioactive compounds such as carotenoids.

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References

Acién FG, Fernández JM, Magán JJ, Molina,E. 2012. Production cost of a real microalgae production plant and strategies to reduce it. Biotechnol. Adv. 30, 1344-1353. https://doi.org/10.1016/j.biotechadv.2012.02.005 PMid:22361647

Borges TH, Pereira JA, Cabrera-Vique C, Seiquer I. 2017. Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers. J. Funct. Foods 37, 209-218. https://doi.org/10.1016/j.jff.2017.07.059

Cavallo C, Piqueras-Fiszman B. 2017. Visual elements of packaging shaping healthiness evaluations of consumers: The case of olive oil. J. Sens. Stud. 32, 1-9. https://doi.org/10.1111/joss.12246

Cerón MC, Campos I, Sánchez JF, Acién FG, Molina E, Fernández-Sevilla JM. 2008. Recovery of lutein from microalgae biomass: Development of a process for Scenedesmus almeriensis biomass. J. Agric. Food Chem. 56, 11761-11766. https://doi.org/10.1021/jf8025875 PMid:19049289

Cichelli A, Pertesana GP. 2004. High-performance liquid chromatographic analysis of chlorophylls, pheophytins and carotenoids in virgin olive oils: Chemometric approach to variety classification. J. Chromatogr. A 1046, 141-146. https://doi.org/10.1016/S0021-9673(04)01061-1

CIE 015:2018, Colorimetry, 4th Edition. CIE Central Bureau, Vienna, 2018. https://cie.co.at/publications/colorimetry-4th-edition https://doi.org/10.25039/TR.015.2018

EFSA. Scientific Opinion on the re-evaluation of mixed carotenes (E 160a (i)) and beta-carotene (E 160a (ii)) as a food additive. 2012. 10. 2593. https://doi.org/10.2903/j.efsa.2012.2593

Fernández-Vázquez R, Stinco CM, Hernanz D, Heredia FJ, Vicario IM. 2013. Colour training and colour differences thresholds in orange juice. Food Qual. Prefer. 30 (2), 320-327. https://doi.org/10.1016/j.foodqual.2013.05.018

Garcia-Oliveira P, Jimenez-Lopez C, Lourenço-Lopes C, Chamorro F, Pereira AG, Carrera-Casais A, Fraga-Corral M, Carpena M, Simal-Gandara J, Prieto MA. 2021. Evolution of flavors in extra virgin olive oil shelf-life. Antioxidants 10, 1-20. https://doi.org/10.3390/antiox10030368 PMid:33671068 PMCid:PMC7997466

Gutiérrez R, Gutiérrez F. 1986. Método rápido para definir el color de los aceites de oliva vírgenes. Grasas Aceites 37, 282-284.

Huang M, Cui G, Melgosa M, Sánchez-Marañón M, Li C, Luo MR, Liu H. 2015. Power functions improving the performance of color-difference formulas. Opt. Express 23, 597-610. https://doi.org/10.1364/OE.23.000597 PMid:25835705

Iddir M, Degerli C, Dingeo G, Desmarchelier C, Schleeh T, Borel P, Larondelle Y, Bohn T. 2019. Whey protein isolate modulates beta-carotene bioaccessibility depending on gastro-intestinal digestion conditions. Food Chem. 291, 157-166. https://doi.org/10.1016/j.foodchem.2019.04.003 PMid:31006454

IOC. International Olive Oil Council [Online]. World olive oil figures-consumption, 2018. Avilable at http://www.internationaloliveoil.org/estaticos/view/131-world-olive-oil-figures?lang=es_ES. Accessed 07.2019.

IOC. International Olive Council [Online]. World olive oil figures - production, 2013. Available at: <http://www.internationaloliveoil.org/estaticos/view/131-world-olive-oil-figures> Accessed 11.02.14.

Issaoui M, Flamini G, Souid S, Bendini A, Barbieri S, Gharbi I, Toschi TG, Cioni PL, Hammami M. 2016. How the addition of spices and herbs to virgin olive oil to produce flavored oils affects consumer acceptance. Nat. Prod. Commun., 11, 775-780 . https://doi.org/10.1177/1934578X1601100619

Limón P. 2017. Aceites de Oliva Vírgenes extra enriquecidos con nuevos antioxidantes procedentes de microalgas. Caracterización físico-química, colorimétrica y ensayos de estabilidad (Tesis doctoral). Universidad de Jaén.

Limón P, Malheiro R, Casal S, Acién-Fernández FG, Fernández-Sevilla JM, Rodrigues N, Cruz R, Bermejo R, Pereira JA. 2015. Improvement of stability and carotenoids fraction of virgin olive oils by addition of microalgae Scenedesmus almeriensis extracts. Food Chem. 175, 203-211. https://doi.org/10.1016/j.foodchem.2014.10.150 PMid:25577071

Luna G, Morales MT, Aparicio R. 2006. Changes induced by UV radiation during virgin olive oil storage. J. Agric. Food Chem. 54, 4790-4794. https://doi.org/10.1021/jf0529262 PMid:16787029

Malheiro R, Rodrigues N, Manzke G, Bento A, Pereira JA, Casal S. 2013. The use of olive leaves and tea extracts as effective antioxidants against the oxidation of soybean oil under microwave heating. Ind. Crops Prod. 44, 37-43. https://doi.org/10.1016/j.indcrop.2012.10.023

Martínez JA, Melgosa M, Pérez MM, Hita E, Negueruela AI. 2001. Visual and instrumental color evaluation in red wines. Food Sci. Technol. Int. 7, 439-444. https://doi.org/10.1177/108201301772660501

Meléndez-Martínez A.J. 2019. An overview of carotenoids, apocarotenoids and vitamin A in agro-food, nutrition, health and disease. Molecular Nutrition and Food Research 63, 1-11. https://doi.org/10.1002/mnfr.201801045 PMid:31189216

Meléndez-Martínez AJ, Mandić AI, Bantis F, Böhm V, Borge GIA, Brnčić M, Bysted A, Cano MP, Dias MG, Elgersma A, Fikselová M, García-Alonso J, Giuffrida D, Gonçalves VSS, Hornero-Méndez D, Kljak K, Lavelli V, Manganaris GA, Mapelli-Brahm P, Marounek M, Olmedilla-Alonso B, Periago-Castón MJ, Pintea A, Sheehan JJ, Tumbas Šaponjac V, Valšíková-Frey M, Meulebroek LV, O'Brien N. 2020. A comprehensive review on carotenoids in foods and feeds: status quo, applications, patents, and research needs. Crit. Rev. Food Sci. Nutr. 0, 1-51. https://doi.org/10.1080/10408398.2020.1867959 PMid:33399015

Melgosa M, Hita E, Romero J, Jiménez del Barco L. 1992. Some classical color differences calculated with new formulas. J. Opt. Soc. Am. A 9, 1247-1253. https://doi.org/10.1364/JOSAA.9.001247

Montoya T, Sato T, Kitaguchi S, Bermejo R, Limón PM, Gómez-Robledo L, Melgosa M, 2018. Colour emotions of Japanese subjects for antioxidant-enriched virgin olive oils. Proc. of the International Colour Association Conference (AIC 2018), pp. 653-657. Lisbon.

Moyano MJ, Melgosa M, Alba J, Hita E, Heredia FJ. 1999. Reliability of the bromthymol blue method for color in virgin olive oils. J. Am. Oil Chem. Soc. 76, 687-692. https://doi.org/10.1007/s11746-999-0160-x

Murillo-Cruz MC, Chova M, Bermejo-Román R. 2021a. Effect of adding fungal β-carotene to picual extra virgin olive oils on their physical and chemical properties. J. Food Process. Preserv. 45, 1-10. https://doi.org/10.1111/jfpp.15186

Murillo-Cruz MC, García-Ruíz AB, Chova-Martínez M, Bermejo-Román R. 2021b. Improvement of Physico-chemical Properties of Arbequina Extra Virgin Olive Oil Enriched with β-Carotene from Fungi. J. Oleo Sci. 70, 459-469. https://doi.org/10.5650/jos.ess20195 PMid:33692234

Nogueira-de-Almeida CA, de Castro GA. 2018. Effects of heat treatment by immersion in household conditions on olive oil as compared to other culinary oils: A descriptive study. Int. J. Food Stud. 7, 89-99. https://doi.org/10.7455/ijfs/7.1.2018.a8

Ozawa Y, Sasaki M, Takahashi N, Kamoshita M, Miyake S, Tsubota K. 2012. Neuroprotective Effects of Lutein in the Retina. Curr. Pharm. Des. 18, 51-56. https://doi.org/10.2174/138161212798919101 PMid:22211688 PMCid:PMC3319923

Peñalvo GC, Robledo VR, Callado CSC, Santander-Ortega M., Castro-Vázquez L, Victoria Lozano M, Arroyo-Jiménez MM. 2016. Improving green enrichment of virgin olive oil by oregano. Effects on antioxidants. Food Chem. 197, 509-515. https://doi.org/10.1016/j.foodchem.2015.11.002 PMid:26616982

Rodriguez-Concepcion M, Avalos J, Bonet ML, Boronat A, Gomez-Gomez L, Hornero-Mendez D, Limon MC, Meléndez-Martínez AJ, Olmedilla-Alonso B, Palou A, Ribot J, Rodrigo MJ, Zacarias L, Zhu C. 2018. A global perspective on carotenoids: Metabolism, biotechnology, and benefits for nutrition and health. Prog. Lipid Res. 70, 62-93. https://doi.org/10.1016/j.plipres.2018.04.004 PMid:29679619

Salmerón JF, Gómez-Robledo L, Carvajal MÁ, Huertas R, Moyano MJ, Gordillo B, Palma AJ, Heredia FJ, Melgosa M. 2012. Measuring the colour of virgin olive oils in a new colour scale using a low-cost portable electronic device. J. Food Eng. 111, 247-254. https://doi.org/10.1016/j.jfoodeng.2012.02.025

Syamila M, Gedi MA, Briars R, Ayed C, Gray DA. 2019. Effect of temperature, oxygen and light on the degradation of β-carotene, lutein and α-tocopherol in spray-dried spinach juice powder during storage. Food Chem. 284, 188-197. https://doi.org/10.1016/j.foodchem.2019.01.055 PMid:30744845

Turner T, Burri BJ, Jamil KM, Jamil M. 2013. The effects of daily consumption of β-cryptoxanthin-rich tangerines and β-carotene-rich sweet potatoes on vitamin A and carotenoid concentrations in plasma and breast milk of Bangladeshi women with low vitamin A status in a randomized controlled trial. Am. J. Clin. Nutr. 98, 1200-1208. https://doi.org/10.3945/ajcn.113.058180 PMid:24004891

Van Hoang D, Pham NM, Lee AH, Tran DN, Binns CW. 2018. Dietary carotenoid intakes and prostate cancer risk: A case-control study from Vietnam. Nutrients 10, 1-11. https://doi.org/10.3390/nu10010070 PMid:29324670 PMCid:PMC5793298

Yu L, Wang Y, Wu G, Jin J, Jin Q, Wang X. 2020. Quality and Composition of Virgin Olive Oils from Indigenous and European Cultivars Grown in China. J. Am. Oil Chem. Soc. 97, 341-353. https://doi.org/10.1002/aocs.12315

World Health Organization. 2014. Global Strategy on Diet, Physical Activity and Health. Promoting fruit and vegetable consumption around the world. Available at: <http://www.who.int//dietphysicalactivity/fruit/en/> Accessed 23.02.2014.