Determination of nutritional health indexes of fresh bovine milk using near infrared spectroscopy




Chemometrics, Conjugated linoleic acid, Dairy fat, Health indexes, Human nutrition


Bovine milk is one of the most complete foods that exist. During the last decades, milk FA have shown to improve human health due to the reduction in risk of cardiovascular disease and related pathologies. The aim of this study was to evaluate the feasibility of near infrared spectroscopy (NIRS) reflectance analysis to predict the nutritional value, fatty acid (FA) composition, and health index of fresh milk from dairy cows of pastoral systems. The prediction of Atherogenicity and Thrombogenicity indexes, along with other FA ratios in fresh milk samples by NIRS were precise and accurate. In addition, the calibration model obtained by NIRS provides an opportunity for the routine quantification of milk’s healthy FA such as omega-3 and conjugated linoleic acid (CLA), with applications in the dairy industry for food labeling, and at the farm level for management of the dairy cow’s diet.


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Andueza D, Rouel J, Chilliard Y, Leroux C, Ferlay A. 2013. Prediction of the goat milk fatty acids by near infrared reflectance spectroscopy. Eur. J. Lipid Sci. Technol. 115, 612- 620.

Cécillon L, Barthés BG, Gomez C, Ertlen D, Genot V, Hedde M, Stevens a, Brun JJ. 2009. Assessment and monitoring of soil quality using near-infrared reflectance spectroscopy (NIRS). Eur. J. Soil Sci. 60, 770-784.

Conzen P. 2006. Multivariate calibration a practical guide for developing methods in the quantitative analytical chemistry BrukerOptik GmbH. Ettlingen, Germany.

Coppa M, Ferlay A, Leroux C, Jestin M, Chilliard Y, Martin B, Andueza D. 2010. Prediction of milk fatty acid composition by near infrared reflectance spectroscopy. Int. Dairy J. 20, 182- 189.

Coppa M, Revello- Chion A, Giaccone D, Ferlay A, Tabacco E, Borreani G. 2014. Comparison of near and medium infrared spectroscopy to predict fatty acid composition on fresh and thawed milk. Food Chem. 150, 40-57. PMid:24360418

Cruz-Hemandez C, Kramer JKG, Kraft J, Santercole V, Or-Rashid M, Deng Z, Dugan MER, Delmonte P, Yurawecz MP. 2006. Systematic analysis of trans and conjugated linoleic acids in the mil k and meat of ruminants. Chemistry. 3, 45-93.

Den Besten G, Van Eunen K, Groen AK, Venema K, Reijngoud DJ, Bakker, B.M., 2013. The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism.J. Lipid Res.54 (9), 2325-2340. PMid:23821742 PMCid:PMC3735932

Den Hartigh, L. 2019. Conjugated Linoleic Acid Effects on Cancer, Obesity, and Atherosclerosis: A Review of Pre-Clinical and Human Trials with Current Perspectives. Nutrients. 11(2), 370. PMid:30754681 PMCid:PMC6413010

Delmonte P, Fardin Kia AR, Kramer JKG, Mossoba MM, Sedisky L, Rader J. 2011. Separation characteristics of fatty acid methy 1 esters using SLB-ILlll, a new ionic liquid coated capillary gas chromatographic column. J. Chromatogr. A. 1218, 545-554. PMid:21176911

Ferlay A, Martin B, Pradel P, Coulon JB, Chilliard Y, 2006. Influence of grass-based diets on milk fatty acid composition and milk lipolytic system in Tarentaise and Montbéliarde cow breeds. J. Dairy Sci. 89, 4026-4041.

Givens DI. 2020. Milk Symposium review: The importance of milk and dairy foods. In the diets of infants, adolescents, pregnant women, adults, and the elderly. J. Dairy Sci. 103 (11), 9681-9699. PMid:33076181

Gómez-Cortés, P., Juárez, M., and de la Fuente, M. A. 2018. Milk fatty acids and potential health benefits: An updated vision. Trends Food Sci. Technol. 81, 1-9.

González-Sáiz JM, Pizarro C, Díez I, Ramírez O, González-Navarro CJ, Sáiz-Abajo MJ, Reyes I. 2007. Monitoring of Alcoholic Fermentation of Onion Juice by NIR Spectroscopy: Valorization of Worthless Onions. J. Agric. Food Chem. 55, 2930-2936. PMid:17373817

Kramer J, Hernandez M, Cruz- Hernandez C, Kraft JY. 2008. Combining results of two GC separations partly achies determination of all cis and trans 16:1, 18:1, 18:2 and 18:3 except CLA isomers of milk fat as demostrated usin ag-ion SPE fractionation. Lipids. 43(3), 259-273. PMid:18214567

Kuczyńska B, Puppel K, Gołębiewski M, Kordyasz M, Grodzki H, Brzozowski P. 2012. Comparison of fat and protein fractions of milk constituents in Montbéliarde and Polish Holstein-Friesian cows from one farm in Poland. Acta Vet. Brno. 81, 139-144.

Llano Suarez P, Soldado A, Gonzalez-Arrojo A, Vicente F, de la Roza-Delgado B. 2018. Rapid on-site monitoring of fatty acid profile in raw milk using a handheld near infrared sensor. J. Food Composit. Anal. 70, 1- 8.

Markey O, Vasilopoulou D, Givens DI, Lovegrove JA. 2014. Dairy and cardiovascular health: Friend or foe?. Nutr. Bulletin. 39, 161-171. PMid:25400508 PMCid:PMC4207191

Marinoni L, Monti L, Barzaghi S, de la Roza-Delgado B. 2013. Quantification of casein fractions and of some of their genetic variants in phosphate buffer by Near Infrared spectroscopy. J. Near Infrared Spectrosc. 2, 385-394.

Morales R, Lanuza F, Subiabre I, Carvajal A, Canto F, Ungerfeld E. 2015. A comparison of milk fatty acid profile among three different dairy production systems in Los Ríos District, Chile. Arch Med Vet. 47, 281-292.

Nantapo CTW, Muchenje V, Hugo A. 2014. Atherogenicity index and health-related fatty acids in different stages of lactation from Friesian, Jersey and Friesian x Jersey cross cow milk under a pature-based dairy system. Food Chem. 146, 127-133. PMid:24176323

Núñez-Sánchez N, Martínez-Marín AL, Polvillo O, Fernández-Cabanás VM, Carrizosa J, Urrutia B, Serradilla JM. 2016. Near Infrared Spectroscopy (NIRS) for the determination of the milk fatty acid profile of goats. Food Chem. 190, 244-252. PMid:26212967

Núñez-Sánchez N, Acuti G, Branciari R, Ranucci D, Haouet N, Olivieri O, Trabalza-Marinucci M. 2020. Estimating fatty acid content and related nutritional indexes in ewe milk using different near infrared instruments. J. Food Composit. Anal. 88, 103427.

Orellana C, Peña F, García A, Perea J, Martos J, Domenech V, Acero R. 2009. Carcass characteristics, fatty acid composition, and meat quality of Criollo Argentino and Braford steers raised on forage in a semi-tropical region of Argentina. Meat Sci. 81, 57-64. PMid:22063962

Park,Y, Pariza, M. 2007. Mechanisms of body fat modulation by conjugated linoleic acid (CLA). Food Res. Internat. 40 (3), 311-323.

Pilarczyk R, Wójcik J, Sablik, P, Czerniak P. 2015. Fatty acid profile and health lipid indices in the raw milk of Simmental and Holstein-Friesian cows from an organic, farm. S. Afr. J. Anim. Sci. 45(1), 30-38.

Pinto M, Rubilar A, Carrasco E, Shun Ah-Hen K, Brito C, Molina LH. 2002. Efecto estacional y del área geográfica en la composición de ácidos grasos en la leche de bovinos. Agro Sur. 30, 75-90.

Porep J, Kammerer D, Reinhold C. 2015. On- line application of near infrared (NIR) spectroscopy in food production. Trends Food Sci. Technol. 46, 211-230.

Preble I, Zhang Z, Kopp R, Garzotto M, Bobe G, Shannon J, Takata J. 2019. Dairy Product Consumption and Prostate Cancer Risk in the United States. Nutrients. 11(7), 1615. PMid:31315238 PMCid:PMC6683061

Reeves JB. 2000. Use of near infrared reflectance spectroscopy. In: J.P.F. D'Mello (ed). Farm animal metabolism an nutrition. CABI Publishing, Wallingford, UK. 185-207.

Rodríguez-Alcalá LM, Harte F, Fontecha J. 2009. Fatty acids profile and CLA isomers content of cow, ewe and goat milks processed by high pressure homogenization. Inno. Food Sci. Emerg. Technol. 10 (1), 32-36.

Salter, AM. 2013. Dietary fatty acids and cardiovascular disease. Animal. 7, 163-171. PMid:23031737

Santos-Silva J, Bessa R.J.B, Santos-Silva F. 2002. Effect of genotype, feeding system and slaughter weight on thequality of light lambsII. Fatty acid composition of meat Livest. Prod. Sci. 77, 187-194.

Shingfield KJ, Capó M, Scollan ND. 2013. Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal. 7, 132-162. PMid:23031638

Simopoulos AP. 2002. The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed. Pharmacother 56, 365-379.

Siri-Tarino PW, Sun Q, Hu FB, Krauss RM. 2010. Meta-analysis of prospective cohort studies evaluating the association of saturated fat with cardiovascular disease. Am. J. Clin. Nutr. 91(3), 535-546. PMid:20071648 PMCid:PMC2824152

Sun XQ, Gibbs SJ. 2012. Diurnal variation in fatty acid profiles in rumen digesta from dairy cows grazing high-quality pasture. Anim. Feed Sci. Tech. 177, 152-160.

Thanh LP, Suksombat W. 2015. Milk yield, composition, and fatty acid profile in dairy cows fed a high concentrate diet blended with oil mixtures rich in polyunsaturated fatty acids. Asian Australas J. Anim. Sci. 28 (6), 796-806. PMid:25925057 PMCid:PMC4412976

Ulbritch TLV, Southgate DAT. 1991. Coronary Heart Disease: Seven Dietary Factors. The Lancet. 338, 985-992.

Vargas-Bello-Pérez E, Fehrmann-Cartes K, Íñiguez-González J, Toro-Mujica P, and Garnsworthy, PC. 2015. Short communication: Chemical composition, fatty acid composition, and sensory characteristics of Chanco cheese from dairy cows supplemented with soybean and hydrogenated vegetable oils. J. Dairy Sci. 98, 1-7. PMid:25465558

Williams P. 2014. The RPD statistic: a tutorial note. NIR News 25, 22-26.



How to Cite

Lobos-Ortega I, Pizarro-Aránguiz N, Urrutia N, Silva-Lemus M, Pavez-Andrades P, Subiabre-Riveros I, Torres-Püschel D. Determination of nutritional health indexes of fresh bovine milk using near infrared spectroscopy. Grasas aceites [Internet]. 2022Jun.13 [cited 2024Jul.25];73(2):e458. Available from:




Funding data

Fundación para la Innovación Agraria
Grant numbers PYT-2018-0274

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