Stabilization of organic extra virgin olive oil using maceration and ultrasound-assisted extraction of natural antioxidants from Artemisia absinthium leaves

A. Bezzezi; M.  Boulares; M.  Arfaoui; O.  Ben Moussa; M. Hassouna

doi:10.3989/gya.0984221

Authors

A. Bezzezi University of Carthage, Technological Innovation and Food Security “LR 22-A6R 01” https://orcid.org/0000-0002-3494-3360
M. Boulares University of Carthage, Technological Innovation and Food Security “LR 22-A6R 01” https://orcid.org/0000-0003-0282-0961
M. Arfaoui University of Carthage, Technological Innovation and Food Security “LR 22-A6R 01” https://orcid.org/0000-0002-4692-6680
O. Ben Moussa University of Carthage, Technological Innovation and Food Security “LR 22-A6R 01” https://orcid.org/0000-0002-0521-9381
M. Hassouna University of Carthage, Technological Innovation and Food Security “LR 22-A6R 01” https://orcid.org/0000-0002-6912-1383

DOI:

https://doi.org/10.3989/gya.0984221

Keywords:

Artemisia absinthium leaves, Enrichment, Maceration, Organic extra virgin olive oil, Ultrasound-assisted extraction

Abstract

Olive oil is the most popular product derived from Olea europaea L. due to its organoleptic characteristics and its beneficial effects on human health. Nowadays, there is a growing interest in olive oil enrichment with medicinal plants. In this regard, Artemisia absinthium L. is an edible source of antioxidants. The aim of the present study was to improve the oxidative stability and the nutritional value of Organic Tunisian Chetoui Olive oil by its enrichment with the phenolic compounds in Artemisia absinthium. The enrichment carried out by maceration and ultrasound-assisted extraction did not affect the organic criteria of olive oil. The comparative study, performed during one year of storage, showed that ultrasound-assisted extraction led to the lowest final peroxide and extinction coefficient values. At the end of storage, this method increased oleic acid content and biophenol contents. Furthermore, the anti-radical activities of enriched samples were higher than the control.

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References

Achat S, Tomao V, Madani K, Chibane M, Elmaataoui M, Dangles O, Chemat F. 2012. Direct enrichment of olive oil in oleuropein by ultrasound-assisted maceration at laboratory and pilot plant scale. Ultrasonics Sonochem. 19, 777-786. https://doi.org/10.1016/j.ultsonch.2011.12.006 PMid:22281378

Allane T, Benamara S. 2019. Determination of reducing power of 56 Algerian plant products using olive (Olea europaea) oil as extraction solvent. Chem. Eng. Communic. 206, 12-21. https://doi.org/10.1080/00986445.2018.1469016

Ammar I, BenAmira A, Khemakem I, Attia H, Ennouri M. 2017. Effect of Opuntia ficus-indica flowers maceration on quality and on heat stability of olive oil. J. Food Sci. Technol. 54, 1502-1510. https://doi.org/10.1007/s13197-017-2581-0 PMid:28559609 PMCid:PMC5430182

Antoniewska A, Rutkowska J, Pineda MM, Adamska A. 2018. Antioxidative, nutritional and sensory properties of muffins with buckwheat flakes and amaranth flour blend partially substituting for wheat flour. LWT 89, 217-223. https://doi.org/10.1016/j.lwt.2017.10.039

AOAC. 2000. Official methods and Recommended Practices of the American Oil Chemists' Society (5th ed.). USA, AOCS Press, Champaign, Illinois

Arfaoui M, Boulares M, Bezzezi A, Ayachi S, Ghrab M, Jouin NE, Hassouna M, Boudiche S. 2021. Effect of the enrichment with natural antioxidants obtained by maceration or ultrasound-assisted extraction from olive leaves on organic extra virgin olive oil. Riv. Ital. Sostanze Grasse 99, 121-129

Boudiche S, Bornaz S, Kachouri F. 2003. La competitivite du secteur de l ' huile d ' olive en Tunisie: prix, qualite et avantage concurrentiel national, 6-14.

Boulares M, Bezzezi A, Arfaoui M, Boulares A, Ghrab M, Ben-Moussa O, Hassouna M, Boudiche S. 2022a. Improvement of Tunisian 'Chemlali' extra virgin olive oil stability with rosemary and laurel herbs and essential oils. Riv. Ital. Sostanze Grasse 99 (2), 131-140.

Chemat F, Rombaut N, Sicaire AG, Meullemiestre A, Fabiano-Tixier AS, Abert-Vian M. 2017. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason. Sonochem. 34, 540-560. https://doi.org/10.1016/j.ultsonch.2016.06.035 PMid:27773280

Dahal P, Bista G, Dahal P. 2021. Phytochemical screening, antioxidant, antibacterial, and antidandruff activities of leaf extract of Artemisia indica. J. Rep. Pharmac. Sci. 10, 231-239. https://doi.org/10.4103/jrptps.JRPTPS_110_20

IOC. 2009. International Olive Council. Determination of biophenols in olive oils by HPLC. COI/T.20, 29, 1-8.

IOC. 2018. International Olive Council. Olive oil sensory analysis method: organoleptic evaluation of virgin olive oil, T.20/Doc, 270-278.

IOC. 2019. International Olive Council. Trade standard applying to olive oils and olive pomace oils, 17.

Iqbal S, Younas U, Chan K W, Zia-Ul-Haq M, Ismail M. 2012. Chemical composition of artemisia annua L. leaves and antioxidant potential of extracts as a function of extraction solvents. Molecules, 17, 6020-6032. https://doi.org/10.3390/molecules17056020 PMid:22614857 PMCid:PMC6268588

ISO 5509. 2000. Animal and vegetable fats and oils, ISO method 5509:2000, geneva, Switzerland

Jaber H, Ayadi M, Makni J, Rigane G, Sayadi S, Bouaziz M. 2012. Stabilization of refined olive oil by enrichment with chlorophyll pigments extracted from Chemlali olive leaves. Eur. J. Lipid Sci. Technol. 114, 1274-1283. https://doi.org/10.1002/ejlt.201100176

Judžentiene A. 2016. Wormwood (Artemisia absinthium L.) oils. Essential Oils in Food Preservation, Flavor and Safety, Ed: Victor R. Preedy. pg. 849-856. https://doi.org/10.1016/B978-0-12-416641-7.00097-3

Malheiro R, Casal S, Teixeira H, Bento A, Pereira J A. 2013. Effect of Olive Leaves Addition during the Extraction Process of Overmature Fruits on Olive Oil Quality. Food Bioproc. Technol. 6, 509-521. https://doi.org/10.1007/s11947-011-0719-z

Nie C, Trimpert J, Moon S, Haag R, Gilmore K, Kaufer B B, Seeberger P H. 2021. In vitro efficacy of Artemisia extracts against SARS-CoV-2. Virology J. 18, 1-7. https://doi.org/10.1186/s12985-021-01651-8 PMid:34496903 PMCid:PMC8424155

Riahi L, Ghazghazi H, Ayari B, Aouadhi C, Klay I, Chograni H, Cherif A, Zoghlami N. 2015. Effect of environmental conditions on chemical polymorphism and biological activities among Artemisia absinthium L. essential oil provenances grown in Tunisia. Indus. Crops Prod. 66, 96-102. https://doi.org/10.1016/j.indcrop.2014.12.036

Şahin S, Sayım E, Bilgin M. 2017. Effect of olive leaf extract rich in oleuropein on the quality of virgin olive oil. J. Food Sci. Technol. 54, 1721-1728. https://doi.org/10.1007/s13197-017-2607-7 PMid:28559631 PMCid:PMC5430204

Sanmartin C, Venturi F, Sgherri C, Nari A, Macaluso M, Flamini G, Quartacci M F, Taglieri I, Andrich G, Zinnai A. 2018. The effects of packaging and storage temperature on the shelf-life of extra virgin olive oil. Heliyon 4, e00888. https://doi.org/10.1016/j.heliyon.2018.e00888 PMid:30417155 PMCid:PMC6218649

Sousa A, Casal S, Malheiro R, Lamas H, Bento A, Pereira J A. 2015. Aromatized olive oils: Influence of flavouring in quality, composition, stability, antioxidants, and antiradical potential. LWT 60, 22-28. https://doi.org/10.1016/j.lwt.2014.08.026

Toma M, Vinatoru M, Paniwnyk L, Mason T J. 2001. Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrasonics Sonochem. 8, 137-142. https://doi.org/10.1016/S1350-4177(00)00033-X PMid:11326609