Optimization of the recovery yield of the enzymatic aqueous extraction of oil from wet açaí decocts using Design of Experiment


  • B. Rodrigues Centre for Valorization of Amazonian Bioactive Compounds (CVACBA), Universidade Federal do Pará (UFPA)
  • C. Herman Centre for Valorization of Amazonian Bioactive Compounds (CVACBA), Universidade Federal do Pará (UFPA)




CCD response surface methodology, Euterpe oleracea, Mixture design


These last decades, açaí oil has been extensively studied for its biological properties and has gained interest from the health industry. It has thus become necessary to develop eco-friendly extraction techniques. The main objective of this study was the use of experimental designs for the maximization of the recovery yield of the enzymatic aqueous extraction process of açaí oil from wet decocts. A Simplex-Lattice Mixture Design was employed for the optimization of the proportion of three commercial enzymatic preparations. Subsequently, a Central Composite Design was used to identify the optimal values for total enzymatic concentration (0.5-4.5%) and extraction time (2-12h). The “Response Surface Methodology” (RSM) revealed that the maximum yield (60.55 ± 5.98%) was obtained using a 0.49:0.25:0.28 ternary mixture of Celluclast 1.5 L, Viscozyme L and Ultrazym AFP-L at a total enzymatic concentration of 2.85% for 10.9 hours. This study concluded that the enzymatic aqueous extraction of açaí oil is an efficient and sustainable process.


Download data is not yet available.


Abdulkarim SM, Lai OM, Muhammad SKS, Long K, Ghazali HM. 2006. Use of enzymes to enhance oil recovery during aqueous extraction of moringa oleifera seed oil. J. Food Lipids 13, 113-130.

Batista CCR, Oliveira MS, Araújo ME, Rodrigues AMC, Botelho JRS, Filho APSS, Machado NT, Junior RNC. 2019. Supercritical CO2 extraction of açai (Euterpe oleracea) berry oil: Yield fatty acids allelopathic activities and determination of phenolic and anthocyanins total compounds in the residual pulp. J. Supercrit. Fluids 107, 364-369. https://doi.org/10.1016/j.supflu.2015.10.006

Bichara CMG, Rogez H. 2011. Chapter 1-Açai (Euterpe oleracea Martius) In: Postharvest biology and technology of tropical and subtropical fruit: Açai to Citrus Cambridge: UK Press pp 1-26.

Darnet S, Serra JL, Rodrigues AMC, Silva LHM. 2011. A high-performance liquid chromatography method to measure tocopherols in assai pulp (Euterpe oleracea). Food Res. Int. 44, 2107-2111.

Dela Cruz FB, Migo VP, Valência SA, Demafelis RB, Alfafara CG, Alcantara AJ. 2007. Enzyme mixture for the extraction of oil from seed of Vutalao (Calophyllum inophyllum). Philipp J. Crop Sci. 32 (3), 17-30.

Domínguez H, Núnez MJ, Lema JM. 1994. Enzymatic pretreatment to enhance oil extraction from fruit and oilseeds: A review. Food Chem. 49, 271-286.

Ferreira ES, Rogez HLG, Herman CANP. 2018 Effect of the combination of enzymatic preparations on the aqueous extraction yield of the oil from the pulp of Euterpe oleracea fruit. Braz. J. Chem. Eng. 35 (4), 1193-1201.

Li H, Song C, ZhouH, Wang N, Cao D. 2010. Optimization of the aqueous enzymatic extraction of wheat germ oil using Response Surface Methodology. J. Am. Oil Chem. Soc. 88 (6), 809-817.

Liu C, Hao L, Chen F, Yang C. 2020. Study on extraction of peanut protein and oil bodies by aqueous enzymatic extraction and characterization of protein. J. Chem. 2020, ID 5148967 11 p.

Magalhães TSSA, Macedo PCO, Converti A, Lima AAD. 2020 The use of Euterpe oleracea Mart. as a new perspective for disease treatment and prevention. Biomolecules 10 (6), 813.

Lubrano C, Robin JR, Khaiat. 1994. A Fatty acids sterol and tocopherol composition of oil from the fruit mesocarp of six palm species in French Guiana. OCL.

Mai HC, Truong V, Debaste F. 2013. Optimization of enzyme-aided extraction of oil rich in carotenoids from gac fruit (Momordica cochinchinensis Spreng). Food Technol. Biotech. 51 (4), 488-499.

Nascimento RJS, Curi S, Assis RA, Freitas SP. 2008. Composição em ácidos graxos do óleo da polpa de açaí extraído com enzimas e com hexano. Rev Bras Frutic 30(2):498-502.

Nguyen HC, Vuong DP, Nguyen NTT, Nguyen NP, Su C-H, Wang F-M, Juan H-J. 2020. Aqueous enzymatic extraction of polyunsaturated fatty acid-rich sacha inchi (Plukenetia volubilis L.) seed oil: An eco-friendly approach. LWT 133, 109992.

Pacheco-Palencia LA, Talcott SM, Talcott ST. 2008. Chemical composition antioxidant properties and thermal stability of a phytochemical enriched oil from açai (Euterpe oleracea Mart). J Agric. Food Chem. 56, 4631-4636.

Ricochon G, Muniglia L. 2010. Influence of enzymes on the oil extraction processes in aqueous media. OCL 17 (6), 356-359.

Rosenthal A, Pyle DL, Niranjan K. 1996. Aqueous and enzymatic process for edible oil extraction. Enzyme Microb. Technol. 19, 402-420.

Silva MP, Cunha VMB, Sousa SHB, Menezes EGO, Bezerra PN, Neto JTF, Filho GNR, Araújo ME, Carvalho Jr RN. 2019. Supercritical CO2 extraction of lyophilized Açaí (Euterpe oleracea Mart.) pulp oil from three municipalities in the state of Pará, Brazil. J. CO 2 Utilization 31, 226-234.

Schauss AG. 2010. Açai (Euterpe oleracea Mart): A macro and nutrient rich palm fruit from the Amazon rain forest with demonstrated bioactivities in vitro and in vivo. In D R Watson & V R Preedy (Eds) Bioactive foods promotion health (pp 479-490) Oxford: Academic Press.

Silvamany H, Jahim JM. 2015. Enhancement of palm oil extraction using cell wall degrading enzyme formulation. Malaysian J. Anal. Sci. 19 (1), 77-87.

Skrovankova S, Sumczynski D, Mlcek J, Jurikova T, Sochor J. 2015 Bioactive compounds and antioxidant activity in different types of berries. Int. J. Mol. Sci. 16 (10), 24673-24706.

Tabtabaei S, Diosady LL. 2013. Aqueous and enzymatic extraction processes for the production of food-grade proteins and industrial oil from dehulled yellow mustard flour. Food Res. Int. 52, 547-556.

Womeni HM, Ndjouenkeu R, Kapseu C, Mbiapo FT, Parmentier M, Fanni J. 2008. Aqueous enzymatic oil extraction from Irvingia gabonensis seed kernels. Eur. J. Lipid Sci. Techno.l 110, 232-238.

Xie M, Dunford NT, Goad C. 2011. Enzymatic extraction of wheat germ oil. J. Am. Oil Chem. Soc. 88, 2015-2021.

Yamaguchi KKL, Pereira LFR, Lamarão CV, Lima ES, Veiga-Junior VF. 2015. Amazon açaí: Chemistry and biological activities: A review. Food Chem. 179, 137-151.

Yusoff MM, Gordon MH, Niranjan K. 2015 Aqueous enzyme assisted oil extraction from oilseeds and emulsion de-emulsifying methods: A review. Trends Food Sci. Technol. 41, 60-82.

Zhang S, Wang Z, Xu SY.2007. Optimization of the aqueous enzymatic extraction of rapeseed oil and protein hydrolysates. J. Am. Oil Chem. Soc. 84, 97-105.



How to Cite

Rodrigues B, Herman C. Optimization of the recovery yield of the enzymatic aqueous extraction of oil from wet açaí decocts using Design of Experiment. Grasas aceites [Internet]. 2023Dec.19 [cited 2024Feb.27];74(4):e528. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/2100