Production of structured lipids by enzymatic transesterification of soybean oil and palm kernel oil in a packed-bed reactor


  • Nelson Moreno Safra Centro de Investigación en Ciencia y Tecnología de Alimentos CICTA Escuela de Química, Universidad Industrial de Santander, Bucaramanga
  • Aide Perea Villamil Centro de Investigación en Ciencia y Tecnología de Alimentos CICTA Escuela de Química, Universidad Industrial de Santander, Bucaramanga



Lipase, Lipozyme, Packed-bed reactor, RM-IM, Structured lipids


Enzymatic synthesis of structured lipids by transesterification of soybean oil with palm kernel oil was evaluated in a packed-bed reactor with a capacity for 500g of enzyme loading. Lipozyme RM-IM was used as catalyst. Substrate blends were passed through the enzyme bed at different flow rates. Transesterification reached a level of 19.6 %, with a maximum calculated productivity of 2344 kg of transesterified oil/kg of immobilized enzyme, a flow rate of 9,36 kg oil/kg enzyme/h. The triacylglycerols formed in major proportion were C40:2, C42:2, C42:3, C44:2, C44:3, C50:3 and C50:4. Stereoespecific analysis of the fat before and after transesterification shows a slight migration of acyl groups. The products obtained by this technology can be applied in the formulation of lipid emulsions for enteral and parenteral nutrition and the food industry.


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Buchgraber M, Ulbert F, Anklam E. 2004. Interlaboratory evaluation of injection techniques for triglyceride analysis of cocoa butter by capillary gas chromatography. J. Chromatog. A. 1036 (2) 197-203. doi:10.1016/j.chroma.2004.03.011

Camacho B, Robles M, Camacho F, et al. 2002. Production of structured triglycerides rich in n-3 polyunsaturated fatty acids by the acidolysis of cod liver oil and caprylic acid in a packed-bed reactor: equilibrium and kinetics. Chem. Eng. Sci. 57, 1237-1249. doi:10.1016/S0009-2509(02)00050-7

Carrín ME, Crapiste GH. 2008. Enzymatic acidolysis of sunflower oil with a palmitic-stearic acid mixture. J. Food Eng. 84 (2) 243-249. doi:10.1016/j.jfoodeng.2007.05.016

Chen CW, Chong CL, Ghazali HM, et al. 2007. Interpretation of triacylglycerol profiles of palm oil, palm kernel oil and their binary blends. Food Chem. 100 (1) 178-191. doi:10.1016/j.foodchem.2005.09.044

Corredor D, Caicedo LA. 2005. Modelos matemáticos para reactores biológicos de lecho empacado (PBR): Una revisión bibliográfica. Ing. Investig. 25 (3) 101-110.

D’Agostine D, Ferraz RC, Gioielli LA, et al., 2001. Lípidos estructurados obtenidos por interesterificación de las mezclas binarias y ternarias de las grasas de palma, semilla de palma y triglicéridos de cadena media. Grasas y Aceites 52 (3-4) 214-221.

Dourtoglou T, Stefanou E, Lalas S, et al. 2001. Regioespecific analysis of fatty acids in triacylglycerols with GC usin 1,3-specific lipase in butanol. Analyst. 126, 1032-1036. doi:10.1039/b100861g

Fomuso LB, Akoh CC. 2002. Lipase-catalyzed acidolysis of olive oil and caprylic acid in a bench-scale packed bed bioreactor. Food Res. Int. 35, 15-21. doi:10.1016/S0963-9969(00)00158-7

IUPAC. 1987. Standard Methods for the Analysis of Oils, Fats and Derivatives. 7th edition. Method 2.301: Preparation of fatty acid methyl esters and Method 2.302: Gas liquid chromatography of fatty acid methyl esters.

Iwasaki Y, Yamane T. 2000. Enzymatic synthesis of structural lipids. J. Mol. Catal. B: Enzym. 10, 129-140. doi:10.1016/S1381-1177(00)00120-X

Kim BH, Akoh CC. 2002. Characteristics of structured lipid prepared by lipase-catalyzed acidolysis of roasted sesame oil and caprylic acid in a bench-scale continuous packed bed reactor. J. Agric. Food Chem. 54 (14) 5132- 5141. doi:10.1021/jf060607k

Lai H, Chen W. 2000. Effects of medium-chain and long-chaing triacylglicerols in pediatric surgical patients. Nutrition. 16 (6) 401-406. doi:10.1016/S0899-9007(00)00268-9

Lai OM, Low CT, Akoh CC. 2005. Lipase Catalyzed acidolysis of palm olein and caprylic acid in a continuous bench-scale packed bed bioreactor. Food Chem. 92 (3) 527-533. doi:10.1016/j.foodchem.2004.08.028

Matulka RA, Noguchi O, Nosaka N. 2006. Safety evaluation of a médium- and long-chain triacylglycerol oil produced from médium-chain triacylglicerols and edible vegetable oil. Food Chem. Toxicol. 44, 1530-1538. doi:10.1016/j.fct.2006.04.004

Moreno N. 2008. Obtención de lípidos estructurados a escala semiindustrial mediante transesterificación con lipasas estereoespecíficas inmovilizadas. Tesis (Doctor en Química). Bucaramanga, Colombia, Universidad Industrial de Santander. Facultad de Ciencias. Escuela de Química.

NOVOZYMES. 2004. Ficha de información: Método para el cálculo de la actividad residual de Lipasas.

Osborn HT, Akoh CC. 2002. Structural lipids-Novel fats with medical, nutraceutical, and food applications. Compr. Rev. Food Sci. and Food Saf. 3, 93-102.

Nielsen NS, Yang T, Xu X, Jacobsen C. 2006. Production and oxidative stability of a human milk fat substitute produced from lard by enzyme technology in a pilot packed-bed reactor. Food Chem. 94 (1) 53-60. doi:10.1016/j.foodchem.2004.10.049

Rønne T, Yang T, Jacobsen C, et al. 2005. Enzymatic interesterification of butterfat with rapeseed oil in a continuous packed bed reactor. J. Agric. Food Chem. 53 (14) 5617-5624. doi:10.1021/jf050646g

Simopoulos AP, Cleland LG. 2003. Omega–6/Omega–3 essential fatty acid ratio: The scientific evidence. World Rev. Nutr. Diet. 92, 1–22. doi:10.1159/000073788

Waitzberg DL, Torrinhas RS, Jacinto TM. 2006. New parenteral lipid emulsions for clinical use. J. Parenter. Enteral Nutr. 30 (4) 351-367. doi:10.1177/0148607106030004351 Wanten GJ, Calder PC. 2007. Immune modulation by parenteral lipid emulsions. Amer. J. Clin. Nutr. 85 (5) 1171-1184.

Willis WM, Lencki RW, Marangoni AG. 1998. Lipid Modification strategies in the production of structured lipids.J.Am.Oil Chem. Soc. 76 443-450. doi:10.1007/s11746-999-0022-6

Xu X, Porsgaard T, Zhang H, et al. 2002. Production of structured lipids in a packed-bed reactor with thermomyces lanuginosa lipase. J. Am. Oil Chem. Soc. 79 (6) 561-565. doi:10.1007/s11746-002-0522-6

Xu X, Fomuso L, Akoh C. 2000. Synthesis of structured triacylglycerols by lipase-catalyzed Acidólisis in a packed bed biorreactor. J. Agric. Food Chem. 48, 3-10. doi:10.1021/jf990836p




How to Cite

Moreno Safra N, Perea Villamil A. Production of structured lipids by enzymatic transesterification of soybean oil and palm kernel oil in a packed-bed reactor. grasasaceites [Internet]. 2008Dec.30 [cited 2022Dec.1];59(4):337-45. Available from: