Comparison of different tests used in mapping the Greek virgin olive oil production for the determination of its total antioxidant capacity

Authors

  • Katerina S. Minioti Chemistry Laboratory, Agricultural University of Athens
  • Constantinos A. Georgiou Chemistry Laboratory, Agricultural University of Athens

DOI:

https://doi.org/10.3989/gya.010508

Keywords:

ABTS, DPPH, Folin-Ciocalteu, Olive oil, Phenolic antioxidants, Total antioxidant capacity

Abstract


This study aims to map the total antioxidant capacity (TAC) of 50 Greek olive oil samples from the 2005-2006 season according to production region and cultivar and to compare the 2, 2’-azino-bis (3-ethylbenzo-thiazoline-6- sulfonic acid (ABTS), 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) and Folin-Ciocalteu tests for use with olive oil. Antioxidant capacities determined in the hydrophilic fraction range between 5.42 - 22.5 mM gallic acid Kg-1 olive oil for the ABTS method and 1.29 - 9.95 mM Kg-1 for the DPPH method while in total, olive oil TAC ranges between 77 - 177 mM Kg-1 as assessed by the DPPH method. The results of total phenol content range between 3.8 and 29.4 mM Kg-1 olive oil. Total phenol content correlates with total antioxidant capacity assessed in the hydrophilic fraction through the DPPH (r = 0.89) and the ABTS (r = 0.69) assays. The hydrophilic fraction DPPH values correlate significantly with the ABTS values (r = 0.81). However, the DPPH values for total olive oil correlate poorly with the ABTS assay, the Folin-Ciocalteu method and the DPPH assay in hydrophilic fraction. Although total phenolic content shows good correlation with ABTS and DPPH values and could serve as a useful indicator for olive oil antioxidant capacity, the use of a battery of tests contributes to better characterization of the antioxidant capacity of olive oil.

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References

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Published

2010-03-30

How to Cite

1.
Minioti KS, Georgiou CA. Comparison of different tests used in mapping the Greek virgin olive oil production for the determination of its total antioxidant capacity. Grasas aceites [Internet]. 2010Mar.30 [cited 2024Mar.29];61(1):45-51. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/619

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