Preparation of sn-2 long-chain polyunsaturated monoacylglycerols from fish oil by hydrolysis with a stereospecific lipase from mucor miehei
DOI:
https://doi.org/10.3989/gya.1999.v50.i2.644Keywords:
Enzymatic hydrolysis, Fish oil, Mucor miehei, sn-2 monoacylglycerols (preparation).Abstract
The preparation of sn-2 eicosapentaenoyi glycerol and sn-2 docosahexaenoyi glycerol by the hydrolysis of fish oil by the sn-1, sn-3 stereo-specific immobilised lipase (Lipozyme IM-20) from mucor miehei is described. Monoacylglycerols obtained after the enzymatic hydrolysis were separated by silver nitrate-coated silicic acid column chromatography Both monoacylglycerols can be individually separated in almost pure form by elution from the column with a solvent mixture. The preparation of sn-2 substituted monoacylglycerols from marine origin allows their utilization as substrates for the synthesis of structured long-chain polyunsaturated fatty acid-containing triacylglycerols at specific positions.
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Copyright (c) 1999 Consejo Superior de Investigaciones Científicas (CSIC)
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