Preparation of sn-2 long-chain polyunsaturated monoacylglycerols from fish oil by hydrolysis with a stereospecific lipase from mucor miehei

Authors

  • Susana Nieto Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile
  • Jorge Gutiérrez Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile
  • Julio Sanhueza Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile
  • Alfonso Valenzuela Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile

DOI:

https://doi.org/10.3989/gya.1999.v50.i2.644

Keywords:

Enzymatic hydrolysis, Fish oil, Mucor miehei, sn-2 monoacylglycerols (preparation).

Abstract


The preparation of sn-2 eicosapentaenoyi glycerol and sn-2 docosahexaenoyi glycerol by the hydrolysis of fish oil by the sn-1, sn-3 stereo-specific immobilised lipase (Lipozyme IM-20) from mucor miehei is described. Monoacylglycerols obtained after the enzymatic hydrolysis were separated by silver nitrate-coated silicic acid column chromatography Both monoacylglycerols can be individually separated in almost pure form by elution from the column with a solvent mixture. The preparation of sn-2 substituted monoacylglycerols from marine origin allows their utilization as substrates for the synthesis of structured long-chain polyunsaturated fatty acid-containing triacylglycerols at specific positions.

Downloads

Download data is not yet available.

Downloads

Published

1999-04-30

How to Cite

1.
Nieto S, Gutiérrez J, Sanhueza J, Valenzuela A. Preparation of sn-2 long-chain polyunsaturated monoacylglycerols from fish oil by hydrolysis with a stereospecific lipase from mucor miehei. Grasas aceites [Internet]. 1999Apr.30 [cited 2024Jul.24];50(2):111-3. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/644

Issue

Section

Research