Comparative study of the obtention of crude and semi-purified lipase extracts from rice bran.
Keywords:Enzyme, Extraction, Lipase, Purification, Rice bran.
Crude lipase extracts from defatted rice bran were obtained using three different extraction solutions: 1; CaCl2 0.01 M; 2; Sacarose 0.6M, KCI 10mM, MgCl2 1mM, Ditiotreitol 2mM and 3; Sacarose 0.4M, Ditiotreitol 2mM. Factors (variables) studied were pH (6.0, 7.0, 8.0) and extraction time (1h, 2h, 3h). Extraction temperature was 4 °C. The extracts obtained presented different lipase activities, and the crude extract obtained with CaCl2 0.01 M was the one with the highest specific activity (12.1mU/mg). Partial purification of this crude extract with acetone and ammonium sulfate resulted in fractions with specific activities of 50.7mU/mg and 63.5mU/mg, respectively.
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