Determination of sterols and triterpenic dialcohols in olive oils using HPLC separation and GC analysis. Standardization of the analytical method.

Arturo Cert; Wenceslao Moreda; Josep García Moreno

doi:10.3989/gya.1997.v48.i4.791

Determination of sterols and triterpenic dialcohols in olive oils using HPLC separation and GC analysis. Standardization of the analytical method.

Authors

Arturo Cert Instituto de la Grasa (C.S.I.C).
Wenceslao Moreda Instituto de la Grasa (C.S.I.C).
Josep García Moreno Agra S.A.

DOI:

https://doi.org/10.3989/gya.1997.v48.i4.791

Keywords:

Collaborative trial Sterols, Uvaol.

Abstract

A method is developed for the analysis of sterols together with triterpenic alcohols (erythrodiol and uvaol) in vegetable oils. The method involves obtention of the unsaponifiable matter, isocratic HPLC separation with refractive index detector, preparation of silylderivatives, and analysis by capillary GC. Four procedures involving GC analysis of sterol and sterol+dialcohol fractions obtained by HPLC and CCF separations are compared. A collaborative trial comparing HPLC-sterols+dialcohols and CCF-sterols (official) methods is carried out. For Δ7-sterols, higher values and better repeatability and reproducibility were observed in the first method, but no differences for the remainder sterols were found. The erythrodiol+uvaol determinations by HPLC-sterols+dialcohols and CCF-sterols+dialcohols (official) methods resulted in similar precision.

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Published

1997-08-30

How to Cite

Cert A, Moreda W, García Moreno J. Determination of sterols and triterpenic dialcohols in olive oils using HPLC separation and GC analysis. Standardization of the analytical method. Grasas aceites [Internet]. 1997Aug.30 [cited 2025May2];48(4):207-18. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/791

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Issue

Vol. 48 No. 4 (1997)

Section

Research

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