Purification of soybean lipoxygenase isoenzyme-1 and characterization of its inhibition by 13-hydroperoxides

M. L. Fauconnier; M. Marlier

doi:10.3989/gya.1996.v47.i4.865

Purification of soybean lipoxygenase isoenzyme-1 and characterization of its inhibition by 13-hydroperoxides

Authors

M. L. Fauconnier U.E. R. de Chimie Genérale et Organique, Faculté Universitaire des Sciences Agronomiques
M. Marlier U.E. R. de Chimie Genérale et Organique, Faculté Universitaire des Sciences Agronomiques

DOI:

https://doi.org/10.3989/gya.1996.v47.i4.865

Keywords:

Hydroperoxide, Linoleic acid, Linolenic acid, Lipoxygenase, Soybean

Abstract

Purification of soybean lipoxygenase isoenzyme-1 was reinvestigated furnishing a new procedure. Different parameters influencing the hydroperoxide production were studied. Our study focused particularly on the enzyme activity inhibition by the reaction products. Dixon plots revealed competitive inhibition with K_i value of 0.45 and 1.5 mM respectively for 13-linoleic and 13-linolenic acid hydroperoxides.

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Published

1996-08-30

How to Cite

Fauconnier ML, Marlier M. Purification of soybean lipoxygenase isoenzyme-1 and characterization of its inhibition by 13-hydroperoxides. Grasas aceites [Internet]. 1996Aug.30 [cited 2025May1];47(4):242-6. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/865

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Issue

Vol. 47 No. 4 (1996)

Section

Research

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