Determination of α-tocopherol in pork with high intramuscular fat content

A. Rey; C. López; M. Scares; B. Isabel

doi:10.3989/gya.1996.v47.i5.877

Determination of α-tocopherol in pork with high intramuscular fat content

Authors

A. Rey Departamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense de Madrid
C. López Departamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense de Madrid
M. Scares Departamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense de Madrid
B. Isabel Departamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense de Madrid

DOI:

https://doi.org/10.3989/gya.1996.v47.i5.877

Keywords:

α-tocopherol (determination), Animal tissue, Comparison of methods, Fat

Abstract

Extraction of α-tocopherol from pork samples with low (3%) (LF) or high (9%) (HF) amount of intramuscular fat have been carried out by three different methods, two of them based in saponification plus extraction of α-tocopherol and the other one without saponification. All samples were spiked with five different amount of α-tocopherol prior to analysis. In LF samples, recovery was in the range 85-95% in all cases, with not significant differences between methods. Recovery was much lower in HF samples when using methods which involve prior saponification of muscle samples (50-60%). Changes in KOH concentration did not improve markedly the recovery. The method based on direct extraction provided much better recovery in HF samples (85- 92%) and consequently is recommended for samples high in fat.

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Published

1996-10-30

How to Cite

Rey A, López C, Scares M, Isabel B. Determination of α-tocopherol in pork with high intramuscular fat content. Grasas aceites [Internet]. 1996Oct.30 [cited 2025May2];47(5):331-4. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/877

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Issue

Vol. 47 No. 5 (1996)

Section

Research

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