Determination of α-tocopherol in pork with high intramuscular fat content

Authors

  • A. Rey Departamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense de Madrid
  • C. López Departamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense de Madrid
  • M. Scares Departamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense de Madrid
  • B. Isabel Departamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense de Madrid

DOI:

https://doi.org/10.3989/gya.1996.v47.i5.877

Keywords:

α-tocopherol (determination), Animal tissue, Comparison of methods, Fat

Abstract


Extraction of α-tocopherol from pork samples with low (3%) (LF) or high (9%) (HF) amount of intramuscular fat have been carried out by three different methods, two of them based in saponification plus extraction of α-tocopherol and the other one without saponification. All samples were spiked with five different amount of α-tocopherol prior to analysis. In LF samples, recovery was in the range 85-95% in all cases, with not significant differences between methods. Recovery was much lower in HF samples when using methods which involve prior saponification of muscle samples (50-60%). Changes in KOH concentration did not improve markedly the recovery. The method based on direct extraction provided much better recovery in HF samples (85- 92%) and consequently is recommended for samples high in fat.

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Published

1996-10-30

How to Cite

1.
Rey A, López C, Scares M, Isabel B. Determination of α-tocopherol in pork with high intramuscular fat content. grasasaceites [Internet]. 1996Oct.30 [cited 2023Oct.3];47(5):331-4. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/877

Issue

Vol. 47 No. 5 (1996)

Section

Research

License

Copyright (c) 1996 Consejo Superior de Investigaciones Científicas (CSIC)

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