Fractionation of phenolase from green table olives (<i>Ascolana tenera var.</i>) by immobilized copper affinity chromatography

V. Sciancalepore; G. De Stefano

doi:10.3989/gya.1995.v46.i4-5.933

Fractionation of phenolase from green table olives (Ascolana tenera var.) by immobilized copper affinity chromatography

Authors

V. Sciancalepore Dipartimento di Scienze e Tecnologie Agro-Alimentari, Ambientan e Microbiologiche - Universitá del Molise
G. De Stefano Dipartimento di Scienze e Tecnologie Agro-Alimentari, Ambientan e Microbiologiche - Universitá del Molise

DOI:

https://doi.org/10.3989/gya.1995.v46.i4-5.933

Keywords:

Ascolana tenera var, Green table olive, Immobilized copper affinity chromatography, Phenolase (fractionation)

Abstract

Phenolase from green table olives (Ascolana tenera var.) was fractionated by immobilized copper affinity chromatography. Four chromatographic fractions with high specific activities were obtained. The highest activity was found for the fraction eluted with L-histidine buffer. The four active fractions were characterized by. their response to both specificity and concentration of the substrate.

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Published

1995-10-30

How to Cite

Sciancalepore V, De Stefano G. Fractionation of phenolase from green table olives (Ascolana tenera var.) by immobilized copper affinity chromatography. Grasas aceites [Internet]. 1995Oct.30 [cited 2025May1];46(4-5):251-4. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/933

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Issue

Vol. 46 No. 4-5 (1995)

Section

Research

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